Environmental
DNA
(eDNA)
sampling
has
emerged
as
a
powerful
approach
for
monitoring
biodiversity
in
the
context
of
diverse
terrestrial
and
aquatic
ecosystems,
broad
potential
both
environmental
surveys
species
management.
eDNA
studies
focusing
on
plant
animal
have
capitalized
analytical
methodologies
developed
originally
metagenomic
microorganisms,
technological
improvements
sample
collection.
However,
while
proliferated
rapidly,
efforts
to
standardize
methods
are
still
nascent.
Critically,
fundamental
data
concerning
distribution
persistence
different
ecological
contexts
lacking,
complicating
confidence
detection
comparability
between
studies.
We
propose
that
key
deficits
could
be
addressed
by
using
well-designed
synthetic
standards
or
tracers,
which
widely
used
fields
ranging
from
medical
diagnostics
hydrology.
In-field
application
standardized
mixture
barcoded
fragments
varying
lengths
concentrations
recovered
concommitantly
with
should
provide
critical
yet
missing
turnover,
well
an
internal
molecular
standard
enhance
Transactions of the American Fisheries Society,
Год журнала:
2023,
Номер
152(6), С. 788 - 808
Опубликована: Окт. 25, 2023
Abstract
Objective
Gill
lice
Salmincola
spp.
are
among
the
most
widely
reported
parasites
of
freshwater
salmonids
and
have
been
associated
with
recent
declines
in
native
Brook
Trout
Salvelinus
fontinalis
populations.
Effective
gill
surveillance
is
vital
for
salmonid
conservation
success,
but
current
survey
approaches
often
inadequate
or
problematic.
We
developed
an
environmental
DNA
(eDNA)‐based
approach
to
detect
louse
edwardsii
explored
viability
this
as
a
management
tool
rapid,
large‐scale
at
Fort
McCoy,
Wisconsin,
USA.
Methods
Reference
sequences
were
generated
from
specimens
collected
McCoy
confirm
their
identify
S.
used
design
quantitative
polymerase
chain
reaction
(qPCR)
assay
that
targets
mitochondrial
cytochrome
c
oxidase
I
(COI).
Assay
validation
included
silico
vitro
sensitivity
nontarget
specificity
testing,
performance
was
validated
situ
by
sampling
eDNA
sites
characterized
via
electrofishing.
Result
Molecular
analysis
reference
confirmed
identity
while
also
providing
first
multi‐population
assessment
genetic
variation
North
American
taxonomically
comprehensive
molecular
phylogeny
Lernaeopodidae
date.
detected
14
38
sites,
including
12
known
active
infestations,
no
evidence
odds
detecting
electrofishing
greater
than
detections
analysis.
presence
highly
predictive
site
occupancy,
concordant
being
obligate
parasite
fishes.
However,
expanding
coverage
analysis,
we
able
isolated
drainage
where
apparently
absent,
confirming
observations.
Conclusion
These
results
suggest
equivalent
terms
yet
offers
substantial
gains
efficiency.
Further,
study
demonstrates
how
can
provide
valuable
intel
directing
efforts,
especially
when
expand
prioritize
follow‐up
conventional
surveys
assess
host–parasite
abundance,
demographics,
status.
Austral journal of veterinary sciences,
Год журнала:
2024,
Номер
unknown, С. e560106 - e560106
Опубликована: Янв. 25, 2024
Water
bodies
constitute
natural,
social,
and
ecological
heritage
under
constant
threat
from
the
footprint
of
human
action.
They
are
habitats
many
species
play
a
key
role
in
sustaining
biodiversity
different
ecosystems.
However,
anthropogenic
activity
can
result
eutrophication
water
bodies,
favoring
excessive
growth
microorganisms,
which
be
risk
factor
for
animal,
human,
environmental
health.
The
Agricultural
Livestock
Service
[Servicio
Agrícola
y
Ganadero,
(SAG)]
Chile
developed
list
Mandatory
Reportable
Diseases
(MRD)
to
report
suspected
contagious
diseases
veterinary
relevance.
In
this
sense,
detection
microorganisms
characterization
their
sanitary
status
is
important
development
monitoring
strategies
prevention
possible
diseases.
This
study
aimed
determine
presence
potentially
harmful
bacteria
Llanquihue
urban
wetlands.
Here,
we
used
metagenomic
sequencing
amplicons
characterize
composition
three
wetlands
city
Llanquihue,
could
animal
Our
main
finding
was
genera
that
may
associated
with
MRD,
addition
other
microbial
have
pathogenic
potential.
On
hand,
also
cyanobacteria
involved
toxin
production
as
another
potential
source
risk.
first
use
massive
techniques
analyze
Llanquihue.
Ecology and Evolution,
Год журнала:
2024,
Номер
14(11)
Опубликована: Ноя. 1, 2024
ABSTRACT
Recent
developments
in
microscopic
and
molecular
tools
have
allowed
the
implementation
of
new
approaches
for
assessing
parasitic
infections
wildlife
populations.
This
is
particularly
important
noninvasive
detection
quantification
endoparasites
live
animals.
Here,
we
combined
copromicroscopic
(Mini‐FLOTAC)
(qPCR)
techniques
to
detect
infection
macroparasite
Ligula
intestinalis
(Cestoda,
Pseudophyllidea)
fresh
droppings
Gull‐billed
Terns
Gelochelidon
nilotica
(Charadriiformes,
Laridae)
breeding
southwestern
Spain.
Additionally,
sequenced
cytochrome
b
gene
parasite
isolates
from
(definitive
host)
Common
Bleak
Alburnus
alburnus
(second
intermediate
sampled
around
tern
colonies
explore
potential
genetic
differences
between
isolates.
The
qPCR
test
showed
a
higher
prevalence
(18%;
13/73
samples)
than
Mini‐FLOTAC
(9%;
8/88
samples),
indicating
that
was
more
sensitive
diagnostic
purposes
fecal
flotation
alone.
Although
agreement
both
substantial
(84.2%)
mainly
due
large
number
uninfected
samples,
only
us
quantify
shedding.
When
combining
techniques,
did
not
differ
adults
chicks,
suggesting
frequent
trophic
transmission
parents
their
offspring
via
food
provisioning.
Phylogenetic
analyses
identified
four
haplotypes
Bleak,
all
which
were
placed
within
European
clade
composed
tapeworms
recovered
exclusively
phylogenetically
derived
cyprinid
fish.
This,
with
short
lifespan
mature
tapeworms,
suggests
became
infected
after
consuming
fish
rather
on
West
African
wintering
grounds.
Altogether,
our
results
represent
first
record
L.
characterization
Iberian
Peninsula.
integrative
coprodiagnostic
protocol
can
be
applied
other
host–parasite
systems,
allowing
researchers
study
helminth
wild
populations
using
approach.
Environmental
DNA
(eDNA)
sampling
has
emerged
as
a
powerful
approach
for
monitoring
biodiversity
in
the
context
of
diverse
terrestrial
and
aquatic
ecosystems,
broad
potential
both
environmental
surveys
species
management.
eDNA
studies
focusing
on
plant
animal
have
capitalized
analytical
methodologies
developed
originally
metagenomic
microorganisms,
technological
improvements
sample
collection.
However,
while
proliferated
rapidly,
efforts
to
standardize
methods
are
still
nascent.
Critically,
fundamental
data
concerning
distribution
persistence
different
ecological
contexts
lacking,
complicating
confidence
detection
comparability
between
studies.
We
propose
that
key
deficits
could
be
addressed
by
using
well-designed
synthetic
standards
or
tracers,
which
widely
used
fields
ranging
from
medical
diagnostics
hydrology.
In-field
application
standardized
mixture
barcoded
fragments
varying
lengths
concentrations
recovered
concommitantly
with
should
provide
critical
yet
missing
turnover,
well
an
internal
molecular
standard
enhance
