Investigative Ophthalmology & Visual Science,
Год журнала:
2024,
Номер
65(2), С. 29 - 29
Опубликована: Фев. 21, 2024
Purpose:
Bacterial
keratitis
(BK)
is
a
serious
ocular
infection
that
can
cause
severe
inflammation
and
corneal
scarring,
leading
to
vision
loss.
In
this
study,
we
aimed
investigate
the
involvement
of
ferroptosis
in
pathogenesis
BK.
Methods:
Transcriptome
analysis
was
performed
evaluate
ferroptosis-related
gene
expression
human
BK
corneas.
Subsequently,
mouse
models
Pseudomonas
aeruginosa
stromal
stem
cells
(CSSCs)
were
validated.
The
mice
treated
with
levofloxacin
(LEV)
or
combined
ferrostatin-1
(LEV+Fer-1).
CSSCs
lipopolysaccharide
(LPS)
LPS
Fer-1.
Inflammatory
cytokines,
α-SMA,
regulators
evaluated
by
RT-qPCR,
immunostaining,
Western
blot.
Iron
reactive
oxygen
species
(ROS)
measured.
Results:
revealed
significant
alterations
genes
models,
group
LEV+Fer-1
exhibited
reduced
inflammatory
cytokines
(MPO,
TNF-α,
IFN-γ),
decreased
scarring
α-SMA
expression,
lower
Fe3+
compared
LEV
groups.
Notably,
showed
elevated
GPX4
SLC7A11
contrast
group.
vitro,
Fer-1
treatment
effectively
restored
ROS,
Fe2+,
GPX4,
induced
CSSCs.
Conclusions:
Ferroptosis
plays
crucial
role
inhibition
holds
promise
for
mitigating
inflammation,
reducing
ultimately
enhancing
prognosis
Consequently,
study
provides
potential
target
innovative
therapeutic
strategies
BK,
which
immense
transform
Biomedicine & Pharmacotherapy,
Год журнала:
2024,
Номер
172, С. 116270 - 116270
Опубликована: Фев. 15, 2024
Iron
homeostasisis
is
integral
to
normal
physiological
and
biochemical
processes
of
lungs.
The
maintenance
iron
homeostasis
involves
the
process
intake,
storage
output,
dependening
on
iron-regulated
protein/iron
response
element
system
operate
tightly
metabolism-related
genes,
including
TFR1,
DMT1,
Fth,
FPN.
Dysregulation
can
lead
overload,
which
increases
virulence
microbial
colonisers
occurrence
oxidative
stress,
causing
alveolar
epithelial
cells
undergo
necrosis
apoptosis,
form
extracellular
matrix.
Accumulated
drive
iron-dependent
ferroptosis
exacerbated
pulmonary
fibrosis.
Notably,
chelator
deferoxamine
lipophilic
antioxidant
ferritin-1
have
been
shown
attenuate
inhibit
lipid
peroxidation
in
paper
summarises
regulatory
mechanisms
dysregulated
metabolism
development
Targeting
may
be
a
potential
therapeutic
strategy
for
prevention
treatment
Abstract
Glaucoma,
a
blind‐leading
disease
largely
since
chronic
pathological
intraocular
high
pressure
(ph‐IOP).
Hitherto,
it
is
reckoned
incurable
for
irreversible
neural
damage
and
challenges
in
managing
IOP.
Thus,
significant
to
develop
neuroprotective
strategies.
Ferroptosis,
initially
identified
as
an
iron‐dependent
regulated
death
that
triggers
Fenton
reactions
culminates
lipid
peroxidation
(LPO),
has
emerged
focal
point
multiple
tumors
neurodegenerative
diseases.
Researches
show
iron
homeostasis
play
critical
roles
the
optic
nerve
(ON)
retinal
ganglion
cells
(RGCs),
suggesting
targeted
treatments
could
be
effective.
In
glaucoma,
apart
from
lesions,
disrupted
metal
balance
increased
oxidative
stress
trabecular
meshwork
(TM)
are
observed.
These
disturbances
lead
extracellular
matrix
excretion
disorders,
known
sclerotic
mechanisms,
resulting
refractory
blockages.
Importantly,
stress,
downstream
effect
of
ferroptosis,
also
key
factor
cell
senescence.
It
plays
crucial
role
both
etiology
risk
glaucoma.
Moreover,
ferroptosis
induces
non‐infectious
inflammation,
which
exacerbate
glaucomatous
injury.
Therefore,
relevance
glaucoma
extensive
multifaceted.
this
review,
study
delves
into
current
understanding
mechanisms
aiming
provide
clues
inform
clinical
therapeutic
practices.
Investigative Ophthalmology & Visual Science,
Год журнала:
2024,
Номер
65(2), С. 29 - 29
Опубликована: Фев. 21, 2024
Purpose:
Bacterial
keratitis
(BK)
is
a
serious
ocular
infection
that
can
cause
severe
inflammation
and
corneal
scarring,
leading
to
vision
loss.
In
this
study,
we
aimed
investigate
the
involvement
of
ferroptosis
in
pathogenesis
BK.
Methods:
Transcriptome
analysis
was
performed
evaluate
ferroptosis-related
gene
expression
human
BK
corneas.
Subsequently,
mouse
models
Pseudomonas
aeruginosa
stromal
stem
cells
(CSSCs)
were
validated.
The
mice
treated
with
levofloxacin
(LEV)
or
combined
ferrostatin-1
(LEV+Fer-1).
CSSCs
lipopolysaccharide
(LPS)
LPS
Fer-1.
Inflammatory
cytokines,
α-SMA,
regulators
evaluated
by
RT-qPCR,
immunostaining,
Western
blot.
Iron
reactive
oxygen
species
(ROS)
measured.
Results:
revealed
significant
alterations
genes
models,
group
LEV+Fer-1
exhibited
reduced
inflammatory
cytokines
(MPO,
TNF-α,
IFN-γ),
decreased
scarring
α-SMA
expression,
lower
Fe3+
compared
LEV
groups.
Notably,
showed
elevated
GPX4
SLC7A11
contrast
group.
vitro,
Fer-1
treatment
effectively
restored
ROS,
Fe2+,
GPX4,
induced
CSSCs.
Conclusions:
Ferroptosis
plays
crucial
role
inhibition
holds
promise
for
mitigating
inflammation,
reducing
ultimately
enhancing
prognosis
Consequently,
study
provides
potential
target
innovative
therapeutic
strategies
BK,
which
immense
transform
