RNA transcriptome analysis of platelets revealed altered platelet responses and the mechanism of thrombocytopenia in SFTS DOI Creative Commons

Yaohui Fang,

Jingyuan Zhang,

Ling Xu

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Окт. 6, 2023

Abstract Background Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne viral hemorrhagic disease caused by infection Dabie bandavirus (SFTS virus, SFTSV). Thrombocytopenia the primary clinical feature of SFTS and significantly associated severity. However, pathological mechanism in remains unclear. Methods Platelets purified from patients were subjected to RNA transcriptome analyses. Differentially expressed genes (DEGs) platelets deceased surviving identified, their functions transcription levels characterized. DEGs related cell death compared COVID-19 dengue patients. The percentage positive for biomarkers pyroptosis, apoptosis, necroptosis, autophagy, ferroptosis was determined flow cytometry. analyses also performed nonlethal SFTSV model mice. representing functional changes mouse characterized, platelet investigated. Functional SFTSV-infected mice determine different mechanisms underlying humans Results Platelet revealed altered functioning suggested active response but not fatal Enhanced neutrophil activation, interferon (IFN) signaling, virus life cycle common responses SFTS. increased histone methylation impaired vesicle organization may be outcome, while enhanced protein transport membrane catabolic process contribute recovery. Moreover, resulted loss via autophagy ferroptosis. Unlike patients, play a role mainly regulating adaptive immunity, as severe that humans. Conclusions This study humans, which are those infected SFTSV. results deepen our understanding pathogenesis provides insights subsequent studies on development novel intervention strategies.

Язык: Английский

Current insights into human pathogenic phenuiviruses and the host immune system DOI Creative Commons
Chuan‐Min Zhou, Zezheng Jiang,

Ning Liu

и другие.

Virulence, Год журнала: 2024, Номер 15(1)

Опубликована: Июль 29, 2024

Phenuiviruses are a class of segmented negative-sense single-stranded RNA viruses, typically consisting three segments that encode four distinct proteins. The emergence pathogenic phenuivirus strains, such as Rift Valley fever phlebovirus (RVFV) in sub-Saharan Africa, Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) East and Southeast Asia, Heartland (HRTV) the United States has presented considerable challenges to global public health recent years. innate immune system plays crucial role initial defense mechanism host against invading pathogens. In addition continued research aimed at elucidating epidemiological characteristics phenuivirus, significant advancements have been made investigating its viral virulence factors (glycoprotein, non-structural protein, nucleoprotein) potential host–pathogen interactions. Specifically, efforts focused on understanding mechanisms evasion, assembly egress, networks involving cells, programmed cell death, inflammation, nucleic acid receptors, etc. Furthermore, plethora technological advancements, including metagenomics, metabolomics, single-cell transcriptomics, proteomics, gene editing, monoclonal antibodies, vaccines, utilized further our pathogenesis responses. Hence, this review aims provide comprehensive overview current recognition, therapeutic approaches during human infections focusing particularly RVFV SFTSV.

Язык: Английский

Процитировано

4

The Impact of Non-Thyroidal Illness Syndromes on The Prognosis and Immune Profile in Severe Fever with Thrombocytopenia Syndrome Patients DOI Creative Commons
Hongxiao Wu,

Yameng Mu,

Yuanni Liu

и другие.

Infection and Drug Resistance, Год журнала: 2025, Номер Volume 18, С. 1439 - 1449

Опубликована: Март 1, 2025

Non-thyroidal illness syndrome (NTIS) is the most common endocrine dysfunction in critically ill patients and often associated with poor prognosis. Thyroid immune cell disturbances are frequently observed severe fever thrombocytopenia (SFTS). This study aims to evaluate impact of NTIS on prognosis SFTS explore relationship between thyroid hormones (THs) profiles. Adult admitted Yantai Qishan Hospital for from January 2023 December no prior history disease were retrospectively recruited. Multivariable regressions used assess associations clinical outcomes. Spearman correlation analysis was conducted relationships cells THs. categorized into four subtypes based different levels FT4 TSH, association mortality further analyzed. Of 84 included study, 62 (73.8%) diagnosed NTIS. Independent risk predictors which may affect include subtype (P =0.002), viral load = 0.029), FT3 0.032), 0.041). exhibited a higher rate compared euthyroid 0.033). revealed that LYM, LYM%, MONO, MONO%, BAS, CD3+T, CD3+T%, Th, Th% positively correlated FT3, FT4, or TSH levels. more likely present coagulation abnormalities (APTT, P 0.005; D-Dimer, < 0.001), liver enzyme (AST, electrolyte imbalances (Sodium, 0.003), elevated LDH increased ɑ-HBDH 0.003). patients, have lower survival patients. The type 3 than those 1.

Язык: Английский

Процитировано

0

Egg exosome miR-145-5p decreases mitochondrial ROS to protect chicken embryo hepatocytes against apoptosis through targeting MAPK10 DOI Creative Commons

Fengdong Zhang,

Yong Han, Fan Li

и другие.

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Год журнала: 2025, Номер 16(1)

Опубликована: Май 23, 2025

Abstract Background Higher embryonic mortality, especially in aged breeding hens, is associated with insufficient hepatic functionality maintaining redox homeostasis. Our previous study demonstrated that egg exosome-derived miRNAs may play a key role modulating oxidation-reduction process, whereas the exact function and mechanism were still poorly understood. The present aimed to investigate roles of exosome dynamic equilibrium free radicals peroxide agents liver, as well demonstrate specific using oxidative stress-challenged hepatocytes. Results Compared 36-week-old decreased hatchability increased mortality observed 65-week-old hens. Meanwhile, older group showed MDA levels SOD GSH-Px activities muscle serum. Embryonic was significantly positively correlated level negatively activity liver. In addition, 363 differentially expressed genes (DEGs) identified 13 (DE-miRNAs) exosomes. These DEGs DE-miRNAs involved oxidoreductase activity, glutathione metabolic MAPK signaling pathway, apoptosis autophagy. miRNA-mRNA network analysis further found targeted by mainly enriched programmed cell death, such Wherein, MAPK10 highest MCC AUC values related level, served target gene miR-145-5p based on dual luciferase reporter experiment correlation analysis. Bioinformatics miR-145-5p/ axis might alleviate generation apoptosis. primary hepatocytes chick embryos, transfection reversed H 2 O -induced mitochondrial ROS increase, , BAX CASP3 overexpression excessive Conclusion Exosome eggs could decrease ROS, attenuating damage embryos. findings provide new theoretical basis for improvement maternal physiological status maintain homeostasis nutritional or genetic modifications. Graphical

Язык: Английский

Процитировано

0

Development and validation of the PLNA score to predict cytokine storm in acute-phase SFTS patients: A single-center cohort study DOI Creative Commons
Zishuai Liu, Ziruo Ge,

Wei Pan

и другие.

International Immunopharmacology, Год журнала: 2024, Номер 136, С. 112288 - 112288

Опубликована: Май 31, 2024

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease known for its high mortality rate and correlation Cytokine Storms (CS). Timely detection of CS crucial improving the prognosis disease. The objective this investigation was to develop a model identifying cytokine storms in acute phase SFTS.

Язык: Английский

Процитировано

2

Construction and validation of a dynamic nomogram using Lasso-logistic regression for predicting the severity of severe fever with thrombocytopenia syndrome patients at admission DOI Creative Commons
Peng Xia, Yu Zhai, Xiaodi Yan

и другие.

BMC Infectious Diseases, Год журнала: 2024, Номер 24(1)

Опубликована: Сен. 18, 2024

Язык: Английский

Процитировано

1

RNA transcriptome analysis of platelets revealed altered platelet responses and the mechanism of thrombocytopenia in SFTS DOI Creative Commons

Yaohui Fang,

Jingyuan Zhang,

Ling Xu

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Окт. 6, 2023

Abstract Background Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne viral hemorrhagic disease caused by infection Dabie bandavirus (SFTS virus, SFTSV). Thrombocytopenia the primary clinical feature of SFTS and significantly associated severity. However, pathological mechanism in remains unclear. Methods Platelets purified from patients were subjected to RNA transcriptome analyses. Differentially expressed genes (DEGs) platelets deceased surviving identified, their functions transcription levels characterized. DEGs related cell death compared COVID-19 dengue patients. The percentage positive for biomarkers pyroptosis, apoptosis, necroptosis, autophagy, ferroptosis was determined flow cytometry. analyses also performed nonlethal SFTSV model mice. representing functional changes mouse characterized, platelet investigated. Functional SFTSV-infected mice determine different mechanisms underlying humans Results Platelet revealed altered functioning suggested active response but not fatal Enhanced neutrophil activation, interferon (IFN) signaling, virus life cycle common responses SFTS. increased histone methylation impaired vesicle organization may be outcome, while enhanced protein transport membrane catabolic process contribute recovery. Moreover, resulted loss via autophagy ferroptosis. Unlike patients, play a role mainly regulating adaptive immunity, as severe that humans. Conclusions This study humans, which are those infected SFTSV. results deepen our understanding pathogenesis provides insights subsequent studies on development novel intervention strategies.

Язык: Английский

Процитировано

0