RNA transcriptome analysis of platelets revealed altered platelet responses and the mechanism of thrombocytopenia in SFTS DOI Creative Commons

Yaohui Fang,

Jingyuan Zhang,

Ling Xu

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 6, 2023

Abstract Background Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne viral hemorrhagic disease caused by infection Dabie bandavirus (SFTS virus, SFTSV). Thrombocytopenia the primary clinical feature of SFTS and significantly associated severity. However, pathological mechanism in remains unclear. Methods Platelets purified from patients were subjected to RNA transcriptome analyses. Differentially expressed genes (DEGs) platelets deceased surviving identified, their functions transcription levels characterized. DEGs related cell death compared COVID-19 dengue patients. The percentage positive for biomarkers pyroptosis, apoptosis, necroptosis, autophagy, ferroptosis was determined flow cytometry. analyses also performed nonlethal SFTSV model mice. representing functional changes mouse characterized, platelet investigated. Functional SFTSV-infected mice determine different mechanisms underlying humans Results Platelet revealed altered functioning suggested active response but not fatal Enhanced neutrophil activation, interferon (IFN) signaling, virus life cycle common responses SFTS. increased histone methylation impaired vesicle organization may be outcome, while enhanced protein transport membrane catabolic process contribute recovery. Moreover, resulted loss via autophagy ferroptosis. Unlike patients, play a role mainly regulating adaptive immunity, as severe that humans. Conclusions This study humans, which are those infected SFTSV. results deepen our understanding pathogenesis provides insights subsequent studies on development novel intervention strategies.

Language: Английский

The Impact of Non-Thyroidal Illness Syndromes on The Prognosis and Immune Profile in Severe Fever with Thrombocytopenia Syndrome Patients DOI Creative Commons
Hongxiao Wu,

Yameng Mu,

Yuanni Liu

et al.

Infection and Drug Resistance, Journal Year: 2025, Volume and Issue: Volume 18, P. 1439 - 1449

Published: March 1, 2025

Non-thyroidal illness syndrome (NTIS) is the most common endocrine dysfunction in critically ill patients and often associated with poor prognosis. Thyroid immune cell disturbances are frequently observed severe fever thrombocytopenia (SFTS). This study aims to evaluate impact of NTIS on prognosis SFTS explore relationship between thyroid hormones (THs) profiles. Adult admitted Yantai Qishan Hospital for from January 2023 December no prior history disease were retrospectively recruited. Multivariable regressions used assess associations clinical outcomes. Spearman correlation analysis was conducted relationships cells THs. categorized into four subtypes based different levels FT4 TSH, association mortality further analyzed. Of 84 included study, 62 (73.8%) diagnosed NTIS. Independent risk predictors which may affect include subtype (P =0.002), viral load = 0.029), FT3 0.032), 0.041). exhibited a higher rate compared euthyroid 0.033). revealed that LYM, LYM%, MONO, MONO%, BAS, CD3+T, CD3+T%, Th, Th% positively correlated FT3, FT4, or TSH levels. more likely present coagulation abnormalities (APTT, P 0.005; D-Dimer, < 0.001), liver enzyme (AST, electrolyte imbalances (Sodium, 0.003), elevated LDH increased ɑ-HBDH 0.003). patients, have lower survival patients. The type 3 than those 1.

Language: Английский

Citations

0
Current insights into human pathogenic phenuiviruses and the host immune system DOI Creative Commons
Chuan‐Min Zhou, Zezheng Jiang,

Ning Liu

et al.

Virulence, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 29, 2024

Phenuiviruses are a class of segmented negative-sense single-stranded RNA viruses, typically consisting three segments that encode four distinct proteins. The emergence pathogenic phenuivirus strains, such as Rift Valley fever phlebovirus (RVFV) in sub-Saharan Africa, Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) East and Southeast Asia, Heartland (HRTV) the United States has presented considerable challenges to global public health recent years. innate immune system plays crucial role initial defense mechanism host against invading pathogens. In addition continued research aimed at elucidating epidemiological characteristics phenuivirus, significant advancements have been made investigating its viral virulence factors (glycoprotein, non-structural protein, nucleoprotein) potential host–pathogen interactions. Specifically, efforts focused on understanding mechanisms evasion, assembly egress, networks involving cells, programmed cell death, inflammation, nucleic acid receptors, etc. Furthermore, plethora technological advancements, including metagenomics, metabolomics, single-cell transcriptomics, proteomics, gene editing, monoclonal antibodies, vaccines, utilized further our pathogenesis responses. Hence, this review aims provide comprehensive overview current recognition, therapeutic approaches during human infections focusing particularly RVFV SFTSV.

Language: Английский

Citations

2
Development and validation of the PLNA score to predict cytokine storm in acute-phase SFTS patients: A single-center cohort study DOI Creative Commons
Zishuai Liu, Ziruo Ge,

Wei Pan

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 136, P. 112288 - 112288

Published: May 31, 2024

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease known for its high mortality rate and correlation Cytokine Storms (CS). Timely detection of CS crucial improving the prognosis disease. The objective this investigation was to develop a model identifying cytokine storms in acute phase SFTS.

Language: Английский

Citations

1
Construction and validation of a dynamic nomogram using Lasso-logistic regression for predicting the severity of severe fever with thrombocytopenia syndrome patients at admission DOI Creative Commons
Peng Xia, Yu Zhai, Xiaodi Yan

et al.

BMC Infectious Diseases, Journal Year: 2024, Volume and Issue: 24(1)

Published: Sept. 18, 2024

Language: Английский

Citations

1
RNA transcriptome analysis of platelets revealed altered platelet responses and the mechanism of thrombocytopenia in SFTS DOI Creative Commons

Yaohui Fang,

Jingyuan Zhang,

Ling Xu

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 6, 2023

Abstract Background Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne viral hemorrhagic disease caused by infection Dabie bandavirus (SFTS virus, SFTSV). Thrombocytopenia the primary clinical feature of SFTS and significantly associated severity. However, pathological mechanism in remains unclear. Methods Platelets purified from patients were subjected to RNA transcriptome analyses. Differentially expressed genes (DEGs) platelets deceased surviving identified, their functions transcription levels characterized. DEGs related cell death compared COVID-19 dengue patients. The percentage positive for biomarkers pyroptosis, apoptosis, necroptosis, autophagy, ferroptosis was determined flow cytometry. analyses also performed nonlethal SFTSV model mice. representing functional changes mouse characterized, platelet investigated. Functional SFTSV-infected mice determine different mechanisms underlying humans Results Platelet revealed altered functioning suggested active response but not fatal Enhanced neutrophil activation, interferon (IFN) signaling, virus life cycle common responses SFTS. increased histone methylation impaired vesicle organization may be outcome, while enhanced protein transport membrane catabolic process contribute recovery. Moreover, resulted loss via autophagy ferroptosis. Unlike patients, play a role mainly regulating adaptive immunity, as severe that humans. Conclusions This study humans, which are those infected SFTSV. results deepen our understanding pathogenesis provides insights subsequent studies on development novel intervention strategies.

Language: Английский

Citations

0