High-fat intake induces gut microbiota disorders, inflammatory responses and oxidative stress in Nyctereutes procyonoides DOI Creative Commons
Zhaoming Li,

Chengwei Wei,

Jie Yang

и другие.

Animal Diseases, Год журнала: 2024, Номер 4(1)

Опубликована: Авг. 6, 2024

Abstract The Nyctereutes procyonoides is highly regarded in the farming and leather industries because of high value its fur, which renders artificial feeding a crucial aspect. However, high-fat diets have always been associated with variety digestive disorders. This study aimed to investigate impact on gut microbiota mechanisms damage . 16S rRNA sequencing demonstrated that caused diarrhea intestinal through alterations microbiota: decrease abundance Firmicutes, an increase Proteobacteria Actinobacteria , Enterococcaceae Escherichia coli-Shigella Clostridium Lactobacillus Subsequently, changes metabolic pathways, such as amino fatty acid were identified by KEGG COG enrichment analysis, TLR4/NF-κB/NLRP3 inflammatory signaling pathway was shown be activated diets. In addition, lead accumulation ROS MDA reduce activity antioxidant enzymes GSH-PX SOD. Correspondingly, levels proinflammatory cytokines (IL-6, IL-1β TNF-α) significantly increased, apoptosis necrosis pathways colonic cells detected, causing dramatic expression tight junction proteins (Occludin, E-cadherin, ZO-1 ZO-2). conclusion, altered structure community led colon damage. provides new insights into health Graphical

Язык: Английский

High-fat diet disrupts the gut microbiome, leading to inflammation, damage to tight junctions, and apoptosis and necrosis in Nyctereutes procyonoides intestines DOI Creative Commons

Chengwei Wei,

Tianchao Xu,

Yuan Geng

и другие.

Microbiology Spectrum, Год журнала: 2024, Номер 12(4)

Опубликована: Фев. 20, 2024

ABSTRACT Given the burgeoning Nyctereutes procyonoides breeding industry and its growing scale, it is imperative to investigate impact of high-fat diets on health these animals. This study involved 30 male comparable weights (3 kg ±0.5), randomly assigned either a control group or diet ( n = 15 each). The latter was fed mixture lard basal in 2:5 ratio, establishing model procyonoides. induced diarrhea histopathological changes Analysis small intestine contents using 16S rRNA sequencing revealed diet-induced disruption gut microbiota. Specifically, Escherichia-Shigella emerged as biomarker P 0.049), while Vagococcus prevalent indicating significant increase harmful bacteria group. Furthermore, this disrupted flora correlated with inflammation oxidative stress, evidenced by marked increases TNF-α < 0.01), IL-1β 0.05), IL-6 0.05) levels, measured via q-PCR, Western blot, stress assays. In addition, q-PCR analysis upregulation apoptosis necrosis markers, including Bax, Caspase3, Caspase9, Caspase12, RIPK3, RIPK1 0.01 0.001), concurrent downregulation anti-apoptotic gene Bcl-2 0.01) group, consistent protein expression trends. These findings suggest that alters microbiome toward more bacterial composition, escalating inflammatory responses intestinal tissue permeability, culminating cell necrosis. IMPORTANCE examines Our research established diet, revealing impacts, such diarrhea, histological anomalies, alterations emphasize importance preventing issues promoting sustainable growth. They highlight microbiota overall animal health.

Язык: Английский

Процитировано

8
Elucidating emerging signaling pathways driving endothelial dysfunction in cardiovascular aging DOI

Anna De Bartolo,

Tommaso Angelone, Carmine Rocca

и другие.

Vascular Pharmacology, Год журнала: 2025, Номер unknown, С. 107462 - 107462

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
Green tea polyphenols inhibit TBBPA-induced lung injury via enhancing antioxidant capacity and modulating the NF-κB pathway in mice DOI

Hongli Lv,

Jingjing Wang, Yuan Geng

и другие.

Food & Function, Год журнала: 2024, Номер 15(7), С. 3411 - 3419

Опубликована: Янв. 1, 2024

Tetrabromobisphenol A (TBBPA) is a global pollutant. When TBBPA absorbed by the body through various routes, it can have wide range of harmful effects on body. Green tea polyphenols (GTPs) act as antioxidants, resisting toxic animals. The and mechanisms GTP oxidative stress, inflammation apoptosis in mouse lung are unknown. Therefore, we established vivo vitro models exposure antagonism using C57 mice A549 cells examined expression factors related to autophagy, apoptosis. results study showed that increase reactive oxygen species (ROS) levels after decreased autophagy-related Beclin1, LC3-II, ATG3, ATG5, ATG7 ATG12 increased p62; stress inhibits autophagy levels. pro-inflammatory IL-1β, IL-6 TNF-α anti-inflammatory factor IL-10 activation NF-κB p65/TNF-α pathway. Bax, caspase-3, caspase-7 caspase-9 Bcl-2 activate apoptosis-related pathways. addition attenuated levels, restored inhibition reduced Our suggest attenuate modulating ROS, reducing increasing attenuating cells. These provide fundamental information for exploring antioxidant mechanism further studying TBBPA.

Язык: Английский

Процитировано

7
Hyaluronic acid-based injectable formulation developed to mitigate metastasis and radiation-induced skin fibrosis in breast cancer treatment DOI
Yu-Chi Wang, Pei‐Wei Shueng,

Chan-Yu Hu

и другие.

Carbohydrate Polymers, Год журнала: 2024, Номер 336, С. 122136 - 122136

Опубликована: Апрель 6, 2024

Язык: Английский

Процитировано

5
Zinc supplementation alleviates oxidative stress to inhibit chronic gastritis via the ROS/NF-κB pathway in a mouse model DOI
Xiaoran Sun, Shuang Xu, Tianjing Liu

и другие.

Food & Function, Год журнала: 2024, Номер 15(13), С. 7136 - 7147

Опубликована: Янв. 1, 2024

Zinc (Zn) is an important trace element; it involved in the regulation and maintenance of many physiological functions organisms has anti-inflammatory antioxidant properties.

Язык: Английский

Процитировано

5
Selenium: 48-year journey of global clinical trials DOI
Yikun Wu, Jun Pei,

Yuangao Xu

и другие.

Molecular and Cellular Biochemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 4, 2025

Язык: Английский

Процитировано

0
Selenium Mitigates Caerulein and LPS-induced Severe Acute Pancreatitis by Inhibiting MAPK, NF-κB, and STAT3 Signaling via the Nrf2/HO-1 Pathway DOI

K Swetha,

Mylanayakanahosahalli Chandrashekar Indumathi,

R Sanchit Kishan

и другие.

Biological Trace Element Research, Год журнала: 2025, Номер unknown

Опубликована: Фев. 5, 2025

Язык: Английский

Процитировано

0
Glycyrrhizin Alleviates the Damage Caused by Zearalenone and Protects the Glandular Stomach of Chickens DOI Creative Commons
Tong Sun,

Fuhan Wang,

Man Qian

и другие.

Animals, Год журнала: 2025, Номер 15(4), С. 489 - 489

Опубликована: Фев. 9, 2025

Zearalenone (ZEA) is a kind of mycotoxin that widely contaminates food and feed poses threat to poultry farming. As natural extract, glycyrrhizin acid (GA) has antioxidant, antibacterial, anti-inflammatory effects. Although studies have revealed the toxic effects ZEA on liver, mechanism by which GA reduces ZEA's protective glandular stomach remains unclear. In order study therapeutic effect tissue damage caused ZEA, we conducted in vivo vitro experiments compare expression inflammation, oxidative stress, apoptosis, necrosis. The results showed can induce inflammation tissues cells, inducing apoptosis addition, alleviate protect cells. Dietary significantly increased antioxidant capacity glandulae inhibited overexpression NFκB/IκB-α its mediated inflammatory response. Moreover, decreased pro-apoptotic factors necrosis factors, thereby alleviating chicken At present, livers lungs been confirmed studies. However, there no stomach. Therefore, purpose this was explore through experimental comparison. may provide some reference for solution contamination.

Язык: Английский

Процитировано

0
Interrogating mediators of single-cell transcriptional changes in the acute damaged cerebral cortex: Insights into endothelial-astrocyte interactions DOI
Caroline de Jager, Eman Soliman, Michelle H. Theus

и другие.

Molecular and Cellular Neuroscience, Год журнала: 2025, Номер unknown, С. 104003 - 104003

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Potential bidirectional regulatory effects of botanical drug metabolites on tumors and cardiovascular diseases based on the PI3K/Akt/mTOR pathway DOI Creative Commons
Shenglin Ma,

Yongmei Liu,

Jie Wang

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Март 24, 2025

Pharmacological interventions targeting the phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) signaling pathway are predominantly employed as anticancer therapies, yet they frequently associated with significant cardiac toxicity. Additionally, PI3K/Akt/mTOR plays a crucial role in treatment cardiovascular diseases, highlighting its dual significance both oncology and cardiology. Therefore, has become an ideal for studying cardioprotection, effects, their Botanical drugs have emerged source developing therapeutic agents cardioprotective often exhibiting bidirectional protective properties. Consequently, this study investigates regulatory influence botanical drug metabolites cardiology via pathway. The research indicated that critical pathogenesis tumors diseases. Ruscogenin, Sulforaphane, Naringenin, Kaempferol, Poncirin, Puerarin can improve cancer by inhibiting phosphorylation levels within cascade. Moreover, also provide effects injury conditions activating dynamics key components pathway, particularly Akt, along functional implications different sites, may offer new strategies insights mitigation cardiotoxicity therapies.

Язык: Английский

Процитировано

0