Mechanisms of assembly and remodelling of the extracellular matrix
Nature Reviews Molecular Cell Biology,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 2, 2024
Язык: Английский
Modification of extracellular matrix proteins by oxidants and electrophiles
Biochemical Society Transactions,
Год журнала:
2024,
Номер
52(3), С. 1199 - 1217
Опубликована: Май 23, 2024
The
extracellular
matrix
(ECM)
is
critical
to
biological
architecture
and
determines
cellular
properties,
function
activity.
In
many
situations
it
highly
abundant,
with
collagens
elastin
being
some
of
the
most
abundant
proteins
in
mammals.
ECM
comprises
multiple
different
protein
species
sugar
polymers,
both
isoforms
post-translational
modifications
(PTMs)
providing
a
large
variety
microenvironments
that
play
key
role
determining
tissue
structure
health.
A
number
PTMs
(e.g.
cross-links)
present
are
integrity
function,
whereas
others
deleterious
associated
cells.
Modifications
induced
by
reactive
oxidants
electrophiles
have
been
reported
accumulate
increasing
age.
This
accumulation
can
be
exacerbated
disease,
particular
those
acute
or
chronic
inflammation,
obesity
diabetes.
likely
due
higher
fluxes
modifying
agents
these
conditions.
this
focused
review,
effects
other
on
discussed,
focus
artery
wall
atherosclerotic
cardiovascular
disease.
generated
components
reviewed,
together
properties
including
adhesion,
proliferation,
migration,
viability,
metabolic
activity,
gene
expression
phenotype.
Increasing
data
indicates
prevalent
human
mammalian
tissues
an
important
disease
development
progression.
Язык: Английский
Peroxidasin is associated with a mesenchymal-like transcriptional phenotype and promotes invasion in metastatic melanoma
Free Radical Biology and Medicine,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Bromine Distribution in Healthy and Diseased Ovarian Tissues Investigated by X‐Ray Fluorescence Microscopy: An Exploratory Study
X-Ray Spectrometry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 14, 2025
ABSTRACT
Bromine
(Br)
is
found
in
nature
primarily
as
part
of
inorganic
salts,
with
a
significant
presence
seawater,
and
also
soil,
contributing
to
its
natural
occurrence
human
animal
diets.
As
an
element
the
body,
bromine
essential
for
health,
particularly
cofactor
enzyme
peroxidase
(PXDN).
This
role
critical
formation
sulfilimine
bonds
within
collagen
IV,
which
are
necessary
basement
membrane
assembly
proper
tissue
development.
During
cross‐linking,
released
into
extracellular
matrix,
underscoring
important
maintaining
structure.
In
our
exploratory
study,
micro‐
nano‐X‐ray
fluorescence
(XRF)
imaging
at
two
different
synchrotron
beamlines
was
used
analyse
Br
distribution
bovine
ovarian
sections,
well
healthy
endometrium
endometriotic
lesions.
be
widely
distributed
both
normal
tissues,
maximal
concentrations
specific
areas
corresponding
follicles
vessel
walls.
Язык: Английский
Peroxidasin is associated with a mesenchymal-like transcriptional phenotype and promotes invasion in metastatic melanoma
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 10, 2024
Abstract
Cutaneous
melanoma
is
a
highly
invasive,
heterogeneous
and
treatment
resistant
cancer.
It’s
ability
to
dynamically
shift
between
transcriptional
states
or
phenotypes
results
in
an
adaptive
cell
plasticity
that
may
drive
cancer
invasion
the
development
of
therapy
resistance.
The
expression
peroxidasin
(PXDN),
extracellular
matrix
peroxidase,
has
been
proposed
be
associated
with
invasive
metastatic
phenotype.
We
have
confirmed
this
association
by
analysing
transcriptomes
70
lines
variable
levels
PXDN
expression.
This
analysis
highlighted
strong
high
undifferentiated
To
assess
functional
role
invasion,
we
performed
knockout
line
(NZM40).
decreased
potential
∼50%
epithelial-mesenchymal
transition
marker
genes
as
determined
RNAseq
substantiated
proteomics
analysis.
Bioinformatics
differentially
expressed
following
decreases
linked
formation,
organisation
degradation
well
signalling
pathways
such
WNT
pathway.
study
provides
compelling
evidence
plays
promoting
mesenchymal-like
Research
Highlights
strongly
Knockout
EMT
concomitant
vast
changes
relevant
many
aspects
biology.
Язык: Английский
Misregulation of bromotyrosine compromises fertility in male Drosophila
Proceedings of the National Academy of Sciences,
Год журнала:
2024,
Номер
121(21)
Опубликована: Май 15, 2024
Biological
regulation
often
depends
on
reversible
reactions
such
as
phosphorylation,
acylation,
methylation,
and
glycosylation,
but
rarely
halogenation.
A
notable
exception
is
the
iodination
deiodination
of
thyroid
hormones.
Here,
we
report
detection
bromotyrosine
its
subsequent
debromination
during
Drosophila
spermatogenesis.
Bromotyrosine
not
evident
when
express
a
native
flavin-dependent
dehalogenase
that
homologous
to
enzyme
responsible
for
iodide
salvage
from
iodotyrosine
in
mammals.
Deletion
or
suppression
dehalogenase-encoding
condet
(
cdt
)
gene
allows
accumulate
with
no
detectable
chloro-
iodotyrosine.
The
presence
mutant
males
disrupts
sperm
individualization
results
decreased
fertility.
Transgenic
expression
late-staged
germ
cells
rescues
this
defect
enhances
tolerance
male
flies
bromotyrosine.
These
are
consistent
halogenation
affecting
spermatogenesis
process
had
previously
eluded
metabolomic,
proteomic,
genomic
analyses.
Язык: Английский
Proteomic insights into the extracellular matrix: a focus on proteoforms and their implications in health and disease
Expert Review of Proteomics,
Год журнала:
2024,
Номер
21(11), С. 463 - 481
Опубликована: Ноя. 1, 2024
Introduction
The
extracellular
matrix
(ECM)
is
a
highly
organized
and
dynamic
network
of
proteins
glycosaminoglycans
that
provides
critical
structural,
mechanical,
biochemical
support
to
cells.
functions
the
ECM
are
directly
influenced
by
conformation
compose
it.
proteoforms,
which
can
result
from
genetic,
transcriptional,
and/or
post-translational
modifications,
adopt
different
conformations
and,
consequently,
confer
structural
properties
functionalities
in
both
physiological
pathological
contexts.
Язык: Английский
Local photo-crosslinking of native tissue matrix regulates cell function
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 10, 2024
Abstract
Within
most
tissues,
the
extracellular
microenvironment
provides
mechanical
cues
that
guide
cell
fate
and
function.
Changes
in
matrix
such
as
aberrant
deposition,
densification
increased
crosslinking
are
hallmarks
of
late-stage
fibrotic
diseases
often
lead
to
organ
dysfunction.
Biomaterials
have
been
widely
used
mimic
properties
study
However,
initiation
fibrosis
has
largely
overlooked,
due
challenges
recapitulating
early
lesions
within
native
microenvironment.
Using
visible
light
mediated
photochemistry,
we
induced
local
stiffening
proteins
ex
vivo
murine
human
tissue.
In
lung
tissue
epithelial
lineage-traced
mice,
mimicked
alveolar
spreading,
differentiation
remodeling.
inhibition
cytoskeletal
tension
or
integrin
engagement
reduced
spreading
differentiation,
resulting
dedifferentiation
deposition.
Our
findings
emphasize
role
remodeling
early-stage
implications
for
disease
modeling
applications
other
tissues.
Язык: Английский