Journal of the American Chemical Society,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 19, 2025
Controlled
liquid-liquid
phase
separation
(LLPS)
plays
an
important
role
in
the
formation
of
a
heterogeneously
structured
extracellular
matrix
(ECM)
consisting
densely
cross-linked
stiff
structures
compartmentalized
loosely
matrix.
Moreover,
mechanical
cues
presented
by
cellular-scale
structural
heterogeneity
ECM
facilitate
mechanotransduction
cells
and
subsequent
cellular
development.
Therefore,
developing
ECM-mimetic
hydrogels
with
as
inductive
cell
carriers
is
highly
desirable
but
challenging.
Inspired
process,
we
capitalized
on
temperature-assisted
LLPS
custom-designed
temperature-responsive
macromer
(TRM)
to
concentrate
compartmentalize
TRM
dense
phase-separated
precursor
solution
while
keeping
gelatin
comacromer
complex
dilute
phase.
The
cross-linking
produces
(micron)-scale
microdomains
covalent
interspersed
cell-adaptable
interdomain
hydrogel
obtained
heterogeneous
hydrogel,
which
solely
bonds,
promotes
extensive
spreading,
microtubule-based
mechanotransduction,
autophagic
flux
encapsulated
human
mesenchymal
stem
(hMSCs),
thereby
enhancing
osteogenesis
bone
regeneration.
Our
findings
not
only
provide
valuable
guidance
for
fabrication
biomaterials
via
LLPS-mediated
assembly
also
shed
light
mechanobiological
mechanism
underlying
regulation
development
ECM.
Cancer
stem
cells
(CSCs)
are
central
to
tumor
progression,
metastasis,
immune
evasion,
and
therapeutic
resistance.
Characterized
by
remarkable
self-renewal
adaptability,
CSCs
can
transition
dynamically
between
stem-like
differentiated
states
in
response
external
stimuli,
a
process
termed
"CSC
plasticity."
This
adaptability
underpins
their
resilience
therapies,
including
checkpoint
inhibitors
adoptive
cell
therapies
(ACT).
Beyond
intrinsic
properties,
reside
specialized
microenvironment—the
CSC
niche—which
provides
immune-privileged
protection,
sustains
stemness,
fosters
suppression.
review
highlights
the
critical
role
of
niche
driving
immunotherapy
resistance,
emphasizing
need
for
integrative
approaches
overcome
these
challenges.
Cell
adhesion
is
a
fundamental
necessity
for
anchorage-dependent
cells
to
thrive
in
the
matrix.
This
process
serves
as
initial
stage
sequence
of
cellular
activities,
which
includes
cell
diffusion,
migration,
proliferation,
and
differentiation.
study
introduces
novel
surface
modification
designed
engineered
enhance
attachment
cholangiocyte
organoids
facilitate
spreading
cholangiocytes
on
polymer
surface.
Our
findings
revealed
that
recruiting
collagen
layer
polydopamine
(PDA)
nanoparticle
coating
poly(lactide-co-trimethylene
carbonate)
(PLATMC)
plays
key
role
attracting
liver
enhancing
migration.
finding
has
promising
results
generating
two-dimensional
(2D)
monolayer
through
organoid
subsequent
integration.
The
innovative
approach
combines
with
culture
technique,
offering
significant
potential
advancing
bile
duct
regenerative
medicine
developing
more
complex
three-dimensional
(3D)
tissues
vitro.
