Abstract
Antibiotic
resistance
is
one
of
the
biggest
challenges
to
public
health.
While
discovery
antibiotics
has
decreased
pathogen-caused
mortality,
overuse
these
drugs
resulted
in
increased
transfer
and
evolution
antibiotic
genes
(ARGs)
bacteria.
ARGs
naturally
occur
wild
bacterial
communities,
but
are
also
found
concentrations
environments
contaminated
by
wastewater
effluent.
Although
such
relatively
well
described
temperate
environments,
little
known
about
distribution
dissemination
Arctic.
We
characterized
microbial
communities
from
aerosols,
lakes
mats
around
a
remote
Arctic
hamlet
using
metagenomic
approaches.
Specific
objectives
were
(i)
compare
across
habitats,
(ii)
characterize
ARG
populations
along
continuum
anthropogenically
influenced
(iii)
identify
viral
origin.
identified
all
habitats
throughout
watershed,
that
most
impacted
area
had
highest
diversity
relative
uncontaminated
sites,
which
may
be
remnant
signal
effluent
inputs
during
20th
century.
we
predominantly
genomes,
our
data
suggests
mimiviruses
harbor
ARGs.
Bioresources and Bioprocessing,
Год журнала:
2025,
Номер
12(1)
Опубликована: Март 5, 2025
The
fermentation
process
of
strong-flavor
Baijiu
represents
a
complex
and
unique
ecosystem,
characterized
by
the
involvement
various
microorganisms
that
drive
intricate
biochemical
reactions,
ultimately
contributing
to
distinct
flavor
profile
Baijiu.
Viruses
may
affect
succession
thus
style
quality
product.
However,
interaction
between
viruses
during
is
still
unclear.
Here
we
combined
viral
metagenomics
amplicon
sequencing,
physicochemical
analysis,
GC-MS
detection
with
temporal
sampling
study
dynamics
microbial
communities,
properties,
compounds
fermentation.
Viral
metagenomic
analysis
revealed
513
operational
taxonomic
units
(vOTUs),
encompassing
34
families.
Principal
coordinates
(PCoA)
demonstrated
significant
differences
in
vOTUs
at
different
stages.
Notably,
community
exhibited
patterns
stages;
it
changed
rapidly
initial
five
days,
similarities
observed
days
10
20.
Volatile
identified
38
components
fermented
grains,
comprising
16
ester
compounds,
11
alcohols,
8
acids,
majority
formed
30.
Spearman's
rank
correlation
Peduoviridae
negative
Gluconobacter.
Genomoviridae
showed
Issatchenkia,
Penicillium,
Monascus.
These
findings
highlight
potential
for
interactions
communities
fermentation,
underscoring
importance
considering
studies
ecology
foods.
Abstract
Background
Dark
pigmented
snow
and
glacier
ice
algae
on
glaciers
sheets
contribute
to
accelerating
melt.
The
biological
controls
these
algae,
particularly
the
role
of
viruses,
remain
poorly
understood.
Giant
classified
under
nucleocytoplasmic
large
DNA
viruses
(NCLDV)
supergroup
(phylum
Nucleocytoviricota),
are
diverse
globally
distributed.
NCLDVs
known
infect
eukaryotic
cells
in
marine
freshwater
environments,
providing
a
control
algal
population
ecosystems.
However,
there
is
very
limited
information
diversity
ecosystem
function
terrestrial
icy
habitats.
Results
In
this
study,
we
investigate
for
first
time
giant
their
host
connections
habitats,
such
as
cryoconite,
dark
ice,
core,
red
green
snow,
genomic
assemblies
five
cultivated
Chlorophyta
algae.
virus
marker
genes
were
present
almost
all
samples;
highest
abundances
recovered
from
assemblies,
followed
by
ice.
variety
active
protists
GrIS
habitats
containing
NCLDV
suggests
that
infection
can
occur
range
hosts.
Metagenomic
data
contained
evidence
metagenome-assembled
genomes
orders
Imitervirales,
Asfuvirales,
Algavirales.
Conclusion
Our
study
highlights
family
signatures
samples
Greenland
sheet.
(GVMAGs)
found
samples,
related
identified
culture
assemblies;
implying
relationship
between
Metatranscriptomic
viral
also
aligned
with
metagenomic
sequences,
suggesting
an
component
microbial
community
potential
“top-down”
protistan
members.
This
reveals
unprecedented
presence
glacial
dominated
Viruses,
Год журнала:
2025,
Номер
17(1), С. 86 - 86
Опубликована: Янв. 11, 2025
Recent
decades
have
seen
growing
attention
on
viruses
in
the
environment
and
their
potential
impacts
as
a
result
of
global
epidemics.
Due
to
diversity
viral
species
along
with
complexity
environmental
host
factors,
virus
extraction
detection
methods
become
key
for
study
ecology.
This
review
systematically
summarises
extracting
detecting
pathogens
from
different
samples
(e.g.,
soil,
water,
faeces,
air)
biological
plants,
animals)
existing
studies,
comparing
similarities
differences,
applicability,
well
advantages
disadvantages
each
method.
Additionally,
this
discusses
future
directions
research
field.
The
aim
is
provide
theoretical
foundation
technical
reference
ecology
research,
facilitating
further
exploration
applications
Environment International,
Год журнала:
2025,
Номер
197, С. 109363 - 109363
Опубликована: Март 1, 2025
Peatlands
are
essential
reservoirs
of
carbon
and
critical
zones
for
the
cycling
greenhouse
gases
on
Earth.
Their
ecological
functions
primarily
governed
by
microbial
communities
inhabiting
them,
which
vary
with
hydrological
conditions.
However,
roles
viruses
in
peatland
ecosystems
remain
poorly
understood
despite
their
abundance
ubiquity.
To
address
this
gap,
viral
communities,
roles,
responses
to
environmental
factors
were
explored
using
viromics,
metatranscriptomics,
physicochemical
property
analyses
nine
peat
sediments
collected
from
various
layers
three
profiles
different
water
table
levels
Dajiuhu
Peatland,
central
China.
This
study
revealed
that
distance
(DWT)
significantly
influenced
composition
function
altering
redox
potential
total
organic
carbon,
turn
affected
methane
(CH4)
concentrations
pore
water.
Furthermore,
a
notable
putative
auxiliary
metabolic
genes
associated
methane,
nitrogen,
sulfur
metabolism
was
identified
DNA
viruses,
community
strongly
regulated
DWT.
Additionally,
functional
related
oxidative
phosphorylation
cysteine
synthesis
detected
first
time
RNA
viruses.
advances
our
comprehension
how
conditions
affect
peatlands,
provides
new
insights
into
impact
CH4
cycle,
serves
as
crucial
reference
future
investigations
