Biomimetic Liver Lobules from Multi‐Compartmental Microfluidics
Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 19, 2024
Engineered
liver
lobule
is
highly
practical
in
hepatic
disease
treatment,
while
constructing
a
3D
biomimetic
with
heterogeneous
architecture
on
large
scale
challenging.
Here,
inspired
by
the
natural
architectural
construction
of
lobules,
lobules
are
proposed
coaxially
through-pores
for
nutrient
exchange
via
microfluidic
technology.
This
multi-channel
chip
made
parallelly
installing
capillaries.
Sodium
alginate
(Alg)
pumped
through
its
central
channel,
Ca
Язык: Английский
MicroSphere 3D Structures Delay Tissue Senescence through Mechanotransduction
ACS Nano,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 9, 2025
The
extracellular
matrix
(ECM)
stores
signaling
molecules
and
facilitates
mechanical
biochemical
in
cells.
However,
the
influence
of
biomimetic
"rejuvenation"
ECM
structures
on
aging-
degeneration-related
cellular
activities
tissue
repair
is
not
well
understood.
We
combined
physical
extrusion
precise
"on–off"
alternating
cross-linking
methods
to
create
anisotropic
biomaterial
microgels
(MicroRod
MicroSphere)
explored
how
they
regulate
cell
nucleus
pulposus
(NP)
their
potential
antidegenerative
effects
intervertebral
discs.
NP
cells
exhibited
aligned
growth
along
surface
MicroRod,
enhanced
proliferation,
reduced
apoptosis.
This
suggests
an
adaptive
response
involving
adhesion
mechanosensing,
which
causes
cytoskeletal
extension
via
environmental
cues.
maintain
nuclear
membrane
integrity
through
YAP/TAZ
pathway,
activates
cGAS-STING
pathway
rectify
aging
mechanisms.
In
vivo,
MicroRod
carries
reduces
inflammatory
factor
protease
secretion
degenerated
discs,
inhibiting
degeneration
promoting
regeneration.
Our
findings
highlight
role
stress
maintaining
activity
antiaging
harsh
environments,
providing
a
foundation
for
further
research
development
biomaterials.
Язык: Английский
Bioinspired spatially ordered multicellular lobules for liver regeneration
Research,
Год журнала:
2025,
Номер
8
Опубликована: Янв. 1, 2025
Cell
therapy
is
a
promising
strategy
for
acute
liver
failure
(ALF),
while
its
therapeutic
efficacy
often
limited
by
cell
loss
and
poor
arrangement.
Here,
inspired
microunits,
we
propose
novel
spatially
ordered
multicellular
lobules
the
ALF
treatment
using
microfluidic
continuous
spinning
technology.
The
microfluidics
with
multiple
microchannels
was
constructed
assembling
parallel
capillaries.
Sodium
alginate
(Alg)
solution
encapsulating
human
umbilical
vein
endothelial
cells
(HUVECs),
hepatocytes,
mesenchymal
stem
(MSCs)
are
introduced
into
middle
channel
6
outer
channels
of
microfluidics,
respectively.
Simultaneously,
Ca
2+
-loaded
solutions
pumped
through
innermost
outermost
channels,
forming
hollow
microfiber
hepatocytes
MSCs
alternately
surrounding
HUVECs.
These
microfibers
could
highly
resemble
cord-like
structure
lobules,
bringing
about
outstanding
liver-like
functions.
We
have
demonstrated
that
in
rats,
our
biomimetic
can
effectively
suppress
excessive
inflammatory
responses,
decrease
necrosis,
promote
regenerative
pathways,
leading
to
satisfied
efficacy.
findings
underscore
potential
treating
related
diseases
improving
traditional
clinical
methods.
Язык: Английский
Artificial Human Blood Vessels for Tissue Engineering
ACS Materials Letters,
Год журнала:
2025,
Номер
unknown, С. 1626 - 1645
Опубликована: Март 27, 2025
Язык: Английский
Sericin-Based 3D High-Precision Biomimetic Microscaffold Fabricated by Laser Direct Writing for Tissue Engineering
Nano Letters,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 16, 2025
In
tissue
engineering,
scaffolds
are
designed
to
mimic
the
extracellular
matrix
(ECM),
creating
three-dimensional
(3D)
microenvironments
that
support
cell
adhesion
and
growth.
However,
precise
fabrication
of
heterogenenous
ECM-mimicking
3D
microstructures
remains
an
unsolved
challenge.
To
address
this,
high-precise
sericin-based
were
developed
via
femtosecond
laser
direct
writing
(FsLDW)
technology.
Chemically
modified
sericin
served
as
a
monomer
in
FsLDW
process,
achieving
nanoscale
precision
enabling
arbitrary
microstructures.
Biomimetic
models,
derived
from
natural
matrices,
employed
construct
bioscaffolds.
These
anisotropic
effectively
supported
directional
growth
differentiation.
This
advancement
greatly
enhances
tissue-engineered
scaffolds,
creation
heterogenenous,
multifunctional
ECM
functional
development
challenges
accurately
simulating
regeneration.
Язык: Английский
Microfluidic 3D printing hydrogels based on fish liver decellularized extracellular matrix for liver regeneration
Smart Medicine,
Год журнала:
2024,
Номер
3(4)
Опубликована: Дек. 1, 2024
Abstract
Liver
tissue
engineering
offers
potential
in
liver
transplantation,
while
the
development
of
hydrogels
for
scalable
scaffolds
incorporating
natural
components
and
effective
functionalities
is
ongoing.
Here,
we
propose
a
novel
microfluidic
3D
printing
hydrogel
derived
from
decellularized
fish
extracellular
matrix
regeneration.
By
decellularizing
combining
it
with
gelatin
methacryloyl,
scaffold
retains
essential
endogenous
growth
factors
such
as
collagen
glycosaminoglycans.
Additionally,
microfluidic‐assisted
technology
enables
precise
modulation
composition
architecture
to
fulfill
clinical
requirements.
Benefiting
source
materials,
exhibit
excellent
biocompatibility
cellular
proliferation
capacity
induced
pluripotent
stem
cell‐derived
hepatocytes
(iPSC‐heps).
Furthermore,
macroscopic
biomechanical
environment
foster
optimal
functional
expression
iPSC‐heps.
Importantly,
post‐transplantation,
significantly
enhance
survival
rates
function
mice
acute
failure,
promoting
regeneration
repair.
These
findings
suggest
that
printed
represent
promising
candidates
transplantation
recovery.
Язык: Английский
Developing biotechnologies in organoids for liver cancer
Biomedical Technology,
Год журнала:
2024,
Номер
9, С. 100067 - 100067
Опубликована: Ноя. 26, 2024
Язык: Английский
Bioprinting of a multi-composition array to mimic intra-tumor heterogeneity of glioblastoma for drug evaluation
Microsystems & Nanoengineering,
Год журнала:
2024,
Номер
10(1)
Опубликована: Дек. 11, 2024
Microextrusion
printing
is
widely
used
to
precisely
manufacture
microdevices,
microphysiological
systems,
and
biological
constructs
that
feature
micropatterns
microstructures
consisting
of
various
materials.
This
method
particularly
useful
for
creating
models
recapitulate
in
vivo-like
cellular
microenvironments.
Although
there
a
recent
demand
high-throughput
data
from
single
vitro
system,
it
remains
challenging
fabricate
multiple
with
small
volume
bioinks
stable
precise
manner
due
the
spreading
evaporation
issues
extruded
hydrogel.
As
time
increases,
bioink
spreads
evaporates,
leading
technical
problems
decrease
resolution
stability,
as
well
affect
3D
culture
space
cell
viability.
In
this
study,
we
describe
novel
microextrusion
bioprinting
technique
stably
multi-composition
array
massive
nanoliter-scale
hydrogel
dots
by
using
multi-bioink
aerosol-based
crosslinking
techniques
prevent
issues.
We
confirmed
aerosol
effectively
prevented
analyzing
morphological
changes
By
adjusting
extruding
ratio
bioinks,
were
able
print
array.
allowed
us
improve
replicates
provide
system.
The
was
applied
intra-tumor
heterogeneity
glioblastoma
assess
temozolomide
efficacy
on
model.
Язык: Английский
Emerging Functional Porous Scaffolds for Liver Tissue Engineering
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 25, 2024
Abstract
Liver
tissue
engineering
holds
promising
in
synthesizing
or
regenerating
livers,
while
the
design
of
functional
scaffold
remains
a
challenge.
Owing
to
intricate
simulation
extracellular
matrix
structure
and
performance,
porous
scaffolds
have
demonstrated
advantages
creating
liver
microstructures
sustaining
functions.
Currently,
various
methods
processes
been
employed
fabricate
scaffolds,
manipulating
properties
morphologies
materials
confer
them
with
unique
supportive
Additionally,
must
also
facilitate
growth
deliver
cells,
possessing
therapeutic
regenerative
effects.
In
this
review,
it
is
initially
outline
typical
procedures
for
fabricating
showcase
microstructures.
Subsequently,
delved
into
forms
cell
loading
including
scaffold‐based,
scaffold‐free,
synergetic
bioassembly
approaches.
Lastly,
utilization
diseases,
offering
significant
insights
future
implications
regeneration
research
explored.
Язык: Английский