Emerging Functional Porous Scaffolds for Liver Tissue Engineering DOI Open Access
Jinglin Wang, Danqing Huang, Dayu Chen

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 25, 2024

Abstract Liver tissue engineering holds promising in synthesizing or regenerating livers, while the design of functional scaffold remains a challenge. Owing to intricate simulation extracellular matrix structure and performance, porous scaffolds have demonstrated advantages creating liver microstructures sustaining functions. Currently, various methods processes been employed fabricate scaffolds, manipulating properties morphologies materials confer them with unique supportive Additionally, must also facilitate growth deliver cells, possessing therapeutic regenerative effects. In this review, it is initially outline typical procedures for fabricating showcase microstructures. Subsequently, delved into forms cell loading including scaffold‐based, scaffold‐free, synergetic bioassembly approaches. Lastly, utilization diseases, offering significant insights future implications regeneration research explored.

Language: Английский

Biomimetic Liver Lobules from Multi‐Compartmental Microfluidics DOI Creative Commons
Danqing Huang, Zhuhao Wu, Ji Wang

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 19, 2024

Engineered liver lobule is highly practical in hepatic disease treatment, while constructing a 3D biomimetic with heterogeneous architecture on large scale challenging. Here, inspired by the natural architectural construction of lobules, lobules are proposed coaxially through-pores for nutrient exchange via microfluidic technology. This multi-channel chip made parallelly installing capillaries. Sodium alginate (Alg) pumped through its central channel, Ca

Language: Английский

Citations

5
MicroSphere 3D Structures Delay Tissue Senescence through Mechanotransduction DOI
Ziang Li, Jincheng Tang, Liang Zhou

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

The extracellular matrix (ECM) stores signaling molecules and facilitates mechanical biochemical in cells. However, the influence of biomimetic "rejuvenation" ECM structures on aging- degeneration-related cellular activities tissue repair is not well understood. We combined physical extrusion precise "on–off" alternating cross-linking methods to create anisotropic biomaterial microgels (MicroRod MicroSphere) explored how they regulate cell nucleus pulposus (NP) their potential antidegenerative effects intervertebral discs. NP cells exhibited aligned growth along surface MicroRod, enhanced proliferation, reduced apoptosis. This suggests an adaptive response involving adhesion mechanosensing, which causes cytoskeletal extension via environmental cues. maintain nuclear membrane integrity through YAP/TAZ pathway, activates cGAS-STING pathway rectify aging mechanisms. In vivo, MicroRod carries reduces inflammatory factor protease secretion degenerated discs, inhibiting degeneration promoting regeneration. Our findings highlight role stress maintaining activity antiaging harsh environments, providing a foundation for further research development biomaterials.

Language: Английский

Citations

0
Bioinspired spatially ordered multicellular lobules for liver regeneration DOI Creative Commons

Jinglin Wang,

Danqing Huang, Haozhen Ren

et al.

Research, Journal Year: 2025, Volume and Issue: 8

Published: Jan. 1, 2025

Cell therapy is a promising strategy for acute liver failure (ALF), while its therapeutic efficacy often limited by cell loss and poor arrangement. Here, inspired microunits, we propose novel spatially ordered multicellular lobules the ALF treatment using microfluidic continuous spinning technology. The microfluidics with multiple microchannels was constructed assembling parallel capillaries. Sodium alginate (Alg) solution encapsulating human umbilical vein endothelial cells (HUVECs), hepatocytes, mesenchymal stem (MSCs) are introduced into middle channel 6 outer channels of microfluidics, respectively. Simultaneously, Ca 2+ -loaded solutions pumped through innermost outermost channels, forming hollow microfiber hepatocytes MSCs alternately surrounding HUVECs. These microfibers could highly resemble cord-like structure lobules, bringing about outstanding liver-like functions. We have demonstrated that in rats, our biomimetic can effectively suppress excessive inflammatory responses, decrease necrosis, promote regenerative pathways, leading to satisfied efficacy. findings underscore potential treating related diseases improving traditional clinical methods.

Language: Английский

Citations

0
Artificial Human Blood Vessels for Tissue Engineering DOI
Yi Zhang,

Lina Lin,

Mengying Niu

et al.

ACS Materials Letters, Journal Year: 2025, Volume and Issue: unknown, P. 1626 - 1645

Published: March 27, 2025

Language: Английский

Citations

0
Sericin-Based 3D High-Precision Biomimetic Microscaffold Fabricated by Laser Direct Writing for Tissue Engineering DOI
Chengfeng Yang, Zexu Zhang, Xuhao Fan

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

In tissue engineering, scaffolds are designed to mimic the extracellular matrix (ECM), creating three-dimensional (3D) microenvironments that support cell adhesion and growth. However, precise fabrication of heterogenenous ECM-mimicking 3D microstructures remains an unsolved challenge. To address this, high-precise sericin-based were developed via femtosecond laser direct writing (FsLDW) technology. Chemically modified sericin served as a monomer in FsLDW process, achieving nanoscale precision enabling arbitrary microstructures. Biomimetic models, derived from natural matrices, employed construct bioscaffolds. These anisotropic effectively supported directional growth differentiation. This advancement greatly enhances tissue-engineered scaffolds, creation heterogenenous, multifunctional ECM functional development challenges accurately simulating regeneration.

Language: Английский

Citations

0
Microfluidic 3D printing hydrogels based on fish liver decellularized extracellular matrix for liver regeneration DOI Creative Commons
Haozhen Ren, Danqing Huang, Mengdi Qiu

et al.

Smart Medicine, Journal Year: 2024, Volume and Issue: 3(4)

Published: Dec. 1, 2024

Abstract Liver tissue engineering offers potential in liver transplantation, while the development of hydrogels for scalable scaffolds incorporating natural components and effective functionalities is ongoing. Here, we propose a novel microfluidic 3D printing hydrogel derived from decellularized fish extracellular matrix regeneration. By decellularizing combining it with gelatin methacryloyl, scaffold retains essential endogenous growth factors such as collagen glycosaminoglycans. Additionally, microfluidic‐assisted technology enables precise modulation composition architecture to fulfill clinical requirements. Benefiting source materials, exhibit excellent biocompatibility cellular proliferation capacity induced pluripotent stem cell‐derived hepatocytes (iPSC‐heps). Furthermore, macroscopic biomechanical environment foster optimal functional expression iPSC‐heps. Importantly, post‐transplantation, significantly enhance survival rates function mice acute failure, promoting regeneration repair. These findings suggest that printed represent promising candidates transplantation recovery.

Language: Английский

Citations

0
Developing biotechnologies in organoids for liver cancer DOI Creative Commons
Yi Hu, Peng Zheng,

Mengdi Qiu

et al.

Biomedical Technology, Journal Year: 2024, Volume and Issue: 9, P. 100067 - 100067

Published: Nov. 26, 2024

Language: Английский

Citations

0
Bioprinting of a multi-composition array to mimic intra-tumor heterogeneity of glioblastoma for drug evaluation DOI Creative Commons
Gihyun Lee, Soo Jee Kim,

Yejin Choi

et al.

Microsystems & Nanoengineering, Journal Year: 2024, Volume and Issue: 10(1)

Published: Dec. 11, 2024

Microextrusion printing is widely used to precisely manufacture microdevices, microphysiological systems, and biological constructs that feature micropatterns microstructures consisting of various materials. This method particularly useful for creating models recapitulate in vivo-like cellular microenvironments. Although there a recent demand high-throughput data from single vitro system, it remains challenging fabricate multiple with small volume bioinks stable precise manner due the spreading evaporation issues extruded hydrogel. As time increases, bioink spreads evaporates, leading technical problems decrease resolution stability, as well affect 3D culture space cell viability. In this study, we describe novel microextrusion bioprinting technique stably multi-composition array massive nanoliter-scale hydrogel dots by using multi-bioink aerosol-based crosslinking techniques prevent issues. We confirmed aerosol effectively prevented analyzing morphological changes By adjusting extruding ratio bioinks, were able print array. allowed us improve replicates provide system. The was applied intra-tumor heterogeneity glioblastoma assess temozolomide efficacy on model.

Language: Английский

Citations

0
Emerging Functional Porous Scaffolds for Liver Tissue Engineering DOI Open Access
Jinglin Wang, Danqing Huang, Dayu Chen

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 25, 2024

Abstract Liver tissue engineering holds promising in synthesizing or regenerating livers, while the design of functional scaffold remains a challenge. Owing to intricate simulation extracellular matrix structure and performance, porous scaffolds have demonstrated advantages creating liver microstructures sustaining functions. Currently, various methods processes been employed fabricate scaffolds, manipulating properties morphologies materials confer them with unique supportive Additionally, must also facilitate growth deliver cells, possessing therapeutic regenerative effects. In this review, it is initially outline typical procedures for fabricating showcase microstructures. Subsequently, delved into forms cell loading including scaffold‐based, scaffold‐free, synergetic bioassembly approaches. Lastly, utilization diseases, offering significant insights future implications regeneration research explored.

Language: Английский

Citations

0