Chemical and Biological Technologies in Agriculture,
Год журнала:
2024,
Номер
11(1)
Опубликована: Дек. 20, 2024
Glandular
trichomes,
which
act
as
the
first
barrier
against
damage
induced
by
insects
and
disease,
can
produce
specialized
metabolites
that
play
important
roles
during
plant
development.
However,
role
played
microRNAs
(miRNAs),
regulate
many
physiological
processes,
trichome
development
is
not
unraveled
in
detail.
In
this
study,
we
performed
RNA
sequencing
(RNA-Seq)
small
assessments
of
tobacco
trichome,
leaf
minus
(leaf-trichome),
tissue
to
improve
our
understanding
miRNA
mechanisms
regulating
Totally,
identified
270
differentially
expressed
miRNAs
(DEMs)
10,430
genes
(DEGs)
between
leaf-trichome
tissues.
DEM
targets
were
mainly
associated
with
hormone
signal
transduction,
plant–pathogen
interactions,
biosynthesis
secondary
metabolites.
Of
these,
1233
miRNA–mRNA
pairs
reverse
expression
patterns.
Next,
used
dual-luciferase
reporter
(LUC)
assays
reveal
several
potential
significantly
inhibited
corresponding
miRNAs,
including
transcription
factors
(TF)
NAC021,
AP2,
MYB36,
WRKY6
TIFY10B.
Further
analysis
showed
miR6155-WRKY6
might
perform
vital
development,
overexpression
miR6155
resulted
decreased
density.
Taken
together,
these
findings
demonstrate
may
be
involved
tobacco,
they
advance
regulation
mediated
help
genetic
engineering
plants.
The Plant Journal,
Год журнала:
2025,
Номер
121(6)
Опубликована: Март 1, 2025
SUMMARY
Late
blight,
caused
by
Phytophthora
infestans
(
P.
),
seriously
compromises
tomato
growth
and
yield.
PAMP‐induced
peptides
(PIPs)
are
secreted
that
act
as
endogenous
elicitors,
triggering
plant
immune
responses.
Our
previous
research
indicated
the
exogenous
application
of
PIP1
from
Solanum
pimpinelifolium
L3708,
named
SpPIP1,
enhances
resistance
to
.
However,
little
is
known
about
roles
additional
family
members
in
In
addition,
there
remains
a
significant
gap
understanding
receptors
SpPIPs
transcription
factors
(TFs)
regulate
signaling
defense,
combination
TFs
defending
against
pathogens
rarely
studied.
This
study
demonstrates
SpPIP‐LIKE1
(SpPIPL1)
also
strengthens
affecting
phenylpropanoid
biosynthesis
pathway.
Both
SpPIP1
SpPIPL1
trigger
defense
responses
manner
dependent
on
RLK7L.
Tomato
plants
overexpressing
precursors
(SpprePIP1
SpprePIPL1)
exhibited
enhanced
expression
pathogenesis‐related
genes,
elevated
H
2
O
ABA
levels,
increased
lignin
accumulation.
Notably,
SpWRKY65
was
identified
transcriptional
activator
SpprePIP1
SpprePIPL1
Disease
assays
gene
analyses
revealed
overexpression
(OEWRKY65)
confers
,
while
wrky65
knockout
led
opposite
effect.
Intriguingly,
transgenic
studies
showed
either
spraying
OEWRKY65
with
or
co‐overexpressing
further
augmented
resistance,
underscoring
potential
stacking
enhancing
disease
resistance.
summary,
this
offers
new
perspectives
controlling
late
blight
developing
varieties
improved
The
results
emphasize
an
eco‐friendly
strategy
for
crop
protection,
laying
theoretical
foundation
advancing
breeding.
Plants,
Год журнала:
2023,
Номер
12(15), С. 2856 - 2856
Опубликована: Авг. 3, 2023
Endogenous
signaling
compounds
are
intermediaries
in
pathways
that
plants
use
to
respond
the
perception
of
harmful
and
beneficial
organisms.
The
plant
elicitor
peptides
(Peps)
important
endogenous
molecules
induce
elements
defense
responses
such
as
hormone
production,
increased
expression
defensive
genes,
activation
phosphorelays,
induction
cell
secondary
messenger
synthesis.
processes
by
which
Peps
confer
resistance
pathogenic
microorganisms
have
been
extensively
studied
Arabidopsis
but
less
known
crop
plants.
Tomato
many
other
solanaceous
an
polypeptide,
systemin,
is
involved
against
herbivorous
insects
necrotrophic
pathogens.
This
paper
explores
similarity
effects
chemical
properties
Pep
systemin
tomato.
Additionally,
relationship
receptor
receptors
explored,
identification
a
second
tomato
literature
called
into
question.
We
suggest
future
directions
for
research
on
crops
during
interactions
with
microbes.
Journal of Agricultural and Food Chemistry,
Год журнала:
2023,
Номер
71(32), С. 12264 - 12279
Опубликована: Авг. 3, 2023
Tomato
is
a
globally
important
horticultural
and
economic
crop,
but
its
productivity
severely
affected
by
various
stresses.
Plant
small
secretory
peptides
have
been
identified
as
crucial
mediators
in
plant
resistance.
Here,
we
conducted
comparative
transcriptome
analysis
the
prePIP1
gene
from
Solanum
pimpinellifolium
(SpprePIP1),
an
ortholog
of
ArabidopsisprePIP1
encoding
precursor
protein
PAMP-induced
SSP
1.
The
expression
level
SpprePIP1
transcriptionally
induced
tomato
upon
infection
with
Phytophthora
infestans
(P.
infestans),
pathogen
responsible
for
late
blight.
Overexpression
resulted
enhanced
resistance
to
P.
infestans.
In
addition,
exogenous
application
SpPIP1,
whether
through
spraying
or
irrigation,
improved
enhancing
transcript
accumulations
pathogenesis-related
proteins,
well
reactive
oxygen
species
jasmonic
acid
(JA)
levels.
Integrated
transcriptomics
metabolomics
revealed
potential
contributions
JA
phenylpropanoid
biosynthesis
SpPIP1-induced
immunity.
Additionally,
SpPIP1
may
strengthen
salt
stress
ABA
signaling
pathway.
Overall,
our
findings
demonstrate
that
positively
regulates
tolerance
stress,
making
it
elicitor
crop
protection
environmentally
friendly
way.
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 29, 2025
Late
blight,
caused
by
Phytophthora
infestans
(P.
infestans),
poses
a
significant
threat
to
tomato
yield
and
quality.
Traditional
disease
control
strategies
rely
heavily
on
frequent
applications
of
chemical
pesticides,
leading
environmental
pollution
the
emergence
pesticide-resistant
pathogens.
This
highlights
urgent
need
for
environmentally
friendly
plant
technologies.
Rapid
alkalinization
factors
(RALFs)
are
secreted
peptides
that
play
crucial
roles
in
defense.
Here,
11
SlRALF
genes
were
identified
tomato.
Bioinformatics
analyses
conducted
characterize
them.
SlRALF2,
which
responded
significantly
P.
infection,
was
chosen
investigation.
Functional
demonstrated
SlRALF2
enhances
resistance
promoting
expression
level
SlPRs
modulating
key
enzyme
activities.
GO
KEGG
influenced
revealed
its
involvement
several
critical
pathways.
Altogether,
our
findings
establish
as
pivotal
component
defense
signaling,
offering
valuable
insights
crop
breeding.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Май 21, 2024
Abstract
Signaling
peptides
act
as
hormones
to
deliver
short-
or
long-distance
intercellular
signals
govern
complex
developmental
processes.
Identifying
endogenous
signaling
is
challenging
due
their
low
abundance
and
the
unknown
cleavage
sites
required
for
release
from
precursor
proteins,
not
mention
investigation
of
evolutionary
roles
across
species.
Consequently,
very
few
were
evolutionarily
characterized
in
vivo
,
especially
peptides.
Here
we
present
current
largest
peptidomic
datasets
six
species
(maize,
camphor
tree,
tomato,
rose
gum,
soybean
poplar),
totaling
12,242
peptides,
selected
all
representative
clades
angiosperms,
including
monocots,
magnoliids,
rosid
eudicots,
asterid
eudicots.
A
sap
peptide
was
found
be
identical
named
ASAP
(
a
ngiosperm
p
eptide),
emerging
most
conserved
family
discovered
thus
far.
rapidly
induces
series
protein
phosphorylation
involved
cascade
previously
reported
regulate
lignin
biosynthesis,
plant
growth
immunity.
Functional
assays
on
activity
demonstrated
its
capability
induction
monolignol
biosynthesis
deposition.
High-throughput
phenomic
analyses
showed
that
significantly
increased
above-
below-ground
biomass.
In
addition,
treatment
enhanced
immunity
reduced
number
galls
egg
masses
against
nematode
invasion.
This
study
provides
insights
into
conservation
functional
significance
mobile
offering
potential
applications
crop
improvement
disease
management
strategies.
