Environmental Science & Technology,
Год журнала:
2015,
Номер
49(16), С. 10190 - 10198
Опубликована: Июль 20, 2015
Determining
the
effects
of
complex
mixtures
environmental
contaminants
poses
many
challenges
within
field
ecotoxicology.
In
this
study,
graded
concentrations
herring
gull
egg
extracts,
collected
from
five
Great
Lakes
breeding
colonies
with
variable
burdens
organohalogen
(OHCs),
were
administered
to
chicken
embryonic
hepatocytes
determine
on
7-ethoxyresorufin-O-deethylase
(EROD)
activity,
porphyrin
accumulation,
and
mRNA
expression.
EROD
activity
accumulation
permitted
ranking
based
efficacy
eliciting
an
aryl
hydrocarbon
receptor-mediated
response.
An
avian
ToxChip
polymerase
chain
reaction
(PCR)
array
provided
more
exhaustive
coverage
in
terms
potential
toxicity
pathways
being
affected,
including
xenobiotic
lipid
metabolism
thyroid
hormone
pathway.
Herring
eggs
Channel
Shelter
Island
(CHSH,
Lake
Huron)
Gull
(GULL,
Michigan)
had
among
highest
OHC
burdens,
extracts
elicited
a
biochemical
transcriptomic
response
greater
than
that
other
three,
less
polluted
colonies.
For
example,
EC50
values
ECthreshold
lower
for
CHSH
GULL
Extracts
altered
15
13
27
genes
PCR
compared
no
eight
contaminated
sites.
The
combination
well-established
vitro
assay,
two
well-characterized
assays,
geographical
discrimination
variably
Lakes.
Such
high-throughput
assays
show
promise
as
cost-effective
tools
determining
toxic
potencies
environment.
