Environmental Pollution, Год журнала: 2017, Номер 233, С. 797 - 805
Опубликована: Ноя. 12, 2017
Язык: Английский
Environmental Pollution, Год журнала: 2017, Номер 233, С. 797 - 805
Опубликована: Ноя. 12, 2017
Язык: Английский
The Science of The Total Environment, Год журнала: 2017, Номер 599-600, С. 934 - 943
Опубликована: Май 11, 2017
Язык: Английский
Процитировано
113The Science of The Total Environment, Год журнала: 2018, Номер 628-629, С. 453 - 466
Опубликована: Фев. 13, 2018
Язык: Английский
Процитировано
113Molecules, Год журнала: 2019, Номер 24(1), С. 163 - 163
Опубликована: Янв. 3, 2019
Expansion in whole genome sequencing and subsequent increase antibiotic resistance targets have paved the way of high throughput qPCR (HT-qPCR) for analyzing hundreds antimicrobial genes (ARGs) a single run. A meta-analysis 51 selected studies is performed to evaluate ARGs abundance trends over last 7 years. WaferGenTM SmartChip found be most widely used HT-qPCR platform among others evaluating ARGs. Up till now around 1000 environmental samples (excluding biological replicates) from different parts world been analyzed on HT-qPCR. Calculated detection frequency normalized (ARGs/16S rRNA gene) reported gut microbiome shown trend low as compared other matrices. Disparities data analysis which are causing difficulties researchers precise interpretation results highlighted possible forward resolving them also suggested. The potential amplification technologies point care or field deployable devices discussed review. Our review has focused updated information regarding role, current status future perspectives resistance.
Язык: Английский
Процитировано
106Water Research, Год журнала: 2021, Номер 196, С. 117052 - 117052
Опубликована: Март 18, 2021
Язык: Английский
Процитировано
94Environmental Pollution, Год журнала: 2020, Номер 269, С. 116133 - 116133
Опубликована: Ноя. 24, 2020
Язык: Английский
Процитировано
91The Science of The Total Environment, Год журнала: 2018, Номер 643, С. 1348 - 1356
Опубликована: Июль 4, 2018
Язык: Английский
Процитировано
88Environmental Pollution, Год журнала: 2020, Номер 264, С. 114752 - 114752
Опубликована: Май 6, 2020
Язык: Английский
Процитировано
88Critical Reviews in Environmental Science and Technology, Год журнала: 2022, Номер 52(24), С. 4395 - 4419
Опубликована: Янв. 16, 2022
Water environments are increasingly recognized as a conduit for the spread of antibiotic resistance, but there is need to standardize resistance monitoring protocols ensure comparability across studies. Quantitative polymerase chain reaction (qPCR) attractive sensitive means quantifying genes (ARGs) and has been applied broadly over past two decades various water matrices. QPCR avoids challenges biases associated with culture-based methods, providing reproducible highly measure ARGs carried bacterial community. However, numerous quality assurance other aspects that be addressed measurements representative comparable Here we conducted critical review identify gene targets most commonly measured by qPCR quantify in surface water, recycled wastewater assess corresponding protocols. Identified monitored samples included sul1, tetA, intI1, given their abundance tendency correlate anthropogenic inputs, vanA blaCTX-M, more rarely detected, clinically-relevant targets. We identified 117 peer-reviewed studies meeting search criteria application these assays matrices interest systematically assessed protocols, including sample collection concentration, DNA extraction, primer/probe specificity, amplification conditions, amplicon length, PCR inhibition evaluation, limit detection/quantification. Gene copy numbers reported were further compared assay matrix. Based on this comprehensive recommend assays, standardized workflows, reporting five target genes.
Язык: Английский
Процитировано
63The Science of The Total Environment, Год журнала: 2022, Номер 828, С. 154303 - 154303
Опубликована: Март 4, 2022
Язык: Английский
Процитировано
46Antibiotics, Год журнала: 2022, Номер 11(12), С. 1700 - 1700
Опубликована: Ноя. 25, 2022
Pharmaceuticals are present as pollutants in several ecosystems worldwide. Despite the reduced concentrations at which they detected, their negative impact on natural biota constitutes a global concern. The consequences of pharmaceuticals’ presence water sources and food have been evaluated with higher detail for human health. However, although most pharmaceuticals detected environment had not designed to act against microorganisms, it is utmost importance understand environmental native microbiota. Microbial communities can suffer serious from environment, may directly public health ecosystem equilibrium. Among this class pollutants, ones that studied more antibiotics. This work aims provide an overview impacts different biofilms, specifically biofilms aquatic engineered systems. alterations caused biofilm function characteristics, well bacteria antimicrobial tolerance consequently associated risks health, also reviewed. information already available topic, need additional data urges assessment emerging microbial potential impacts.
Язык: Английский
Процитировано
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