Nitrogen removal pathway and dynamics of microbial community with the increase of salinity in simultaneous nitrification and denitrification process

The Science of The Total Environment, Год журнала: 2019, Номер 697, С. 134047 - 134047

Опубликована: Авг. 23, 2019

Язык: Английский

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Full substitution of chemical fertilizer by organic manure decreases soil N₂O emissions driven by ammonia oxidizers and gross nitrogen transformations

Global Change Biology, Год журнала: 2023, Номер 29(24), С. 7117 - 7130

Опубликована: Окт. 6, 2023

Replacing synthetic fertilizer by organic manure has been shown to reduce emissions of nitrous oxide (N2 O), but the specific roles ammonia oxidizing microorganisms and gross nitrogen (N) transformation in regulating N2 O remain unclear. Here, we examined effect completely replacing chemical with on emissions, oxidizers, N rates using a 13-year field manipulation experiment. Our results showed that reduced cumulative 16.3%-210.3% compared fertilizer. The abundance bacteria (AOB) was significantly lower during three growth stages maize. Organic also decreased AOB alpha diversity changed their community structure. However, substitution increased archaea comammox Nitrospira Interestingly, mineralization 23.2%-32.9%, autotrophic nitrification rate 10.5%-45.4%, when This study found positive correlation between abundance, emission, contribution emission supported random forest analysis. highlights key oxidizers predicting cropland O.

Язык: Английский

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High functional diversity among Nitrospira populations that dominate rotating biological contactor microbial communities in a municipal wastewater treatment plant

The ISME Journal, Год журнала: 2020, Номер 14(7), С. 1857 - 1872

Опубликована: Апрель 24, 2020

Abstract Nitrification, the oxidation of ammonia to nitrate via nitrite, is an important process in municipal wastewater treatment plants (WWTPs). Members Nitrospira genus that contribute complete (comammox) have only recently been discovered and their relevance engineered water systems poorly understood. This study investigated distributions Nitrospira, ammonia-oxidizing archaea (AOA), bacteria (AOB) biofilm samples collected from tertiary rotating biological contactors (RBCs) a WWTP Guelph, Ontario, Canada. Using quantitative PCR (qPCR), 16S rRNA gene sequencing, metagenomics, our results demonstrate species strongly dominate RBC comammox outnumber all other nitrifiers. Genome bins recovered assembled metagenomes reveal multiple populations with distinct spatial temporal distributions, including several taxa are previously characterized members. Diverse functional profiles imply high level niche heterogeneity among contrast sole detected AOA representative was cultivated same biofilm. Our metagenome also two cyanase-encoding suggesting ability degrade cyanate, which has shown for representatives strict nitrite oxidizers. demonstrates importance RBCs as model continued investigation environmental factors control activities AOB, AOA,

Язык: Английский

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Does environmental decentralization exacerbate China's carbon emissions? Evidence based on dynamic threshold effect analysis

The Science of The Total Environment, Год журнала: 2020, Номер 721, С. 137656 - 137656

Опубликована: Март 3, 2020

Язык: Английский

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Comammox Nitrospira within the Yangtze River continuum: community, biogeography, and ecological drivers

The ISME Journal, Год журнала: 2020, Номер 14(10), С. 2488 - 2504

Опубликована: Июнь 18, 2020

Abstract The recent discovery of comammox Nitrospira as complete nitrifiers has fundamentally renewed perceptions nitrogen cycling in natural and engineered systems, yet little is known about the environmental controls on these newly recognized bacteria. Based improved phylogenetic resolution through successful assembly ten novel genomes (71–96% completeness), we provided first biogeographic patterns for planktonic benthic Yangtze River over a 6030 km continuum. Our study revealed widespread distributions relative abundance this large freshwater system, constituting 30 46% ammonia-oxidizing prokaryotes (AOPs) displaying 30.4- 17.9-fold greater abundances than canonical representatives water sediments, respectively. Comammox contributed more to nitrifier (34–87% AOPs) typical oligotrophic environments with higher pH lower temperature, particularly plateau (clade B), mountain foothill A) areas upper reach. dominant position was replaced by sublineages I/II bacteria from downstream plain due selection, while dissimilarity moderately associated geographic distance. A substantial decrease (83%) occurred immediately Three Gorges Dam, consistent similarly considerable overall sediment bacterial taxa. Together, highlights previously unrecognized dominance major river systems underlines importance revisiting nitrification processes within global environments.

Язык: Английский

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Dominance of comammox Nitrospira in soil nitrification

The Science of The Total Environment, Год журнала: 2021, Номер 780, С. 146558 - 146558

Опубликована: Март 19, 2021

Язык: Английский

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Comammox Nitrospira Species Dominate in an Efficient Partial Nitrification–Anammox Bioreactor for Treating Ammonium at Low Loadings

Environmental Science & Technology, Год журнала: 2021, Номер 55(3), С. 2087 - 2098

Опубликована: Янв. 13, 2021

Bacteria capable of complete ammonia oxidation (comammox) are widespread and contribute to nitrification in wastewater treatment facilities. However, their roles partial nitrification-anaerobic ammonium (anammox) systems remain unclear. In this study, a bench-scale bioreactor with continuous stirring was operated for more than 1000 days limited oxygen supply achieve efficient nitrogen removal (70.1 ± 2.7%) at low loading 35.2 mg-N/L/day. High-throughput amplicon sequencing analysis the comammox monooxygenase subunit A (amoA) gene revealed seven sequence types from two clusters clade Nitrospira. Quantitative polymerase chain reaction analyses suggested that species dominated ammonia-oxidizing community, an abundance as high 89.2 7.9% total prokaryotic amoA copies. Multiple linear regression further substantial contribution Nitrospira bioreactor. The investigation batch experiments consistently showed activities were inhibited by free far severely other microbes. Overall, study provided new insight into ecology under hypoxic conditions comammox-associated nitrification-anammox potential method treating low-strength ammonium-containing wastewater.

Язык: Английский

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Biogeographic distribution of comammox bacteria in diverse terrestrial habitats

The Science of The Total Environment, Год журнала: 2020, Номер 717, С. 137257 - 137257

Опубликована: Фев. 11, 2020

Язык: Английский

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Distribution and Diversity of Comammox Nitrospira in Coastal Wetlands of China

Frontiers in Microbiology, Год журнала: 2020, Номер 11

Опубликована: Окт. 6, 2020

Complete ammonia oxidizers (comammox), able to individually oxidize nitrate, are considered play a significant role in the global nitrogen cycle. However, distribution of comammox Nitrospira estuarine tidal flat wetland and environmental drivers affecting their abundance diversity remain unknown. Here, we present large-scale investigation on geographical along wetlands China, where were successfully detected 9 16 sampling sites. The ranged from 4.15 × 105 6.67 106 copies/g, 2.21- 5.44-folds lower than canonical oxidizers: ammonia-oxidizing bacteria (AOB) archaea (AOA). Phylogenetic analysis based alpha subunit monooxygenase encoding gene (amoA) revealed that Clade A, mainly originating upstream river inputs, accounts for more 80% Nitrospira, whereas clade B rarely detected. Comammox dominant OTUs varied within samples, showing pattern. Salinity pH most important wetlands. was further negatively correlated with high nitrite concentrations. Altogether, this study existence, driving factors ecosystems, thus providing insights into ecological niches recently discovered nitrifying consortium contributions nitrification environments.

Язык: Английский

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Identification, interactions, nitrogen removal pathways and performances of culturable heterotrophic nitrification-aerobic denitrification bacteria from mariculture water by using cell culture and metagenomics

The Science of The Total Environment, Год журнала: 2020, Номер 732, С. 139268 - 139268

Опубликована: Май 8, 2020

Язык: Английский

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