Contrasting response of comammox Nitrospira, ammonia oxidising bacteria, and archaea to soil pH and nitrogen inputs DOI Creative Commons

C. Chisholm,

Hong J. Di, Keith C. Cameron

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 924, P. 171627 - 171627

Published: March 11, 2024

This study aimed to investigate the effect of soil pH change, and nitrogen amendment on ammonia oxidiser abundance comammox Nitrospira community composition. The experimental design used mesocosms placed in a temperature-controlled incubator for 90 days. A Templeton silt loam was as its physiochemical properties are typical region's dairy farms. results showed that clade B preferred natural (pH 6.1–6.2) with no applied nitrogen. Furthermore, synthetic urine (N700) decreased B. may have been because large amounts available N700 treatments inhibited growth Nitrospira. These suggest while present New Zealand farm soils, but their role nitrification very high environment under patch grazed pastures be limited. Further research is needed confirm this. In contrast comammox, AOB (dominated by Nitrosospira) responded positively application urine. response greatest (7.1), followed then low (4.9) soils. due difference availability. At pH, ammonia/ammonium equilibrium favours production. Calculated availability accurately predicted amoA gene abundance. Interestingly, AOA (which predominantly made up Thaumarchaeota group I.1b E) seemed prefer soils over pH. specific lineage present. did not respond

Language: Английский

Nitrogen removal pathway and dynamics of microbial community with the increase of salinity in simultaneous nitrification and denitrification process DOI
Zhengang Xia, Qun Wang,

Zonglian She

et al.

The Science of The Total Environment, Journal Year: 2019, Volume and Issue: 697, P. 134047 - 134047

Published: Aug. 23, 2019

Language: Английский

Citations

222
High functional diversity among Nitrospira populations that dominate rotating biological contactor microbial communities in a municipal wastewater treatment plant DOI Creative Commons
Emilie Spasov, Jackson M. Tsuji,

Laura Hug

et al.

The ISME Journal, Journal Year: 2020, Volume and Issue: 14(7), P. 1857 - 1872

Published: April 24, 2020

Abstract Nitrification, the oxidation of ammonia to nitrate via nitrite, is an important process in municipal wastewater treatment plants (WWTPs). Members Nitrospira genus that contribute complete (comammox) have only recently been discovered and their relevance engineered water systems poorly understood. This study investigated distributions Nitrospira, ammonia-oxidizing archaea (AOA), bacteria (AOB) biofilm samples collected from tertiary rotating biological contactors (RBCs) a WWTP Guelph, Ontario, Canada. Using quantitative PCR (qPCR), 16S rRNA gene sequencing, metagenomics, our results demonstrate species strongly dominate RBC comammox outnumber all other nitrifiers. Genome bins recovered assembled metagenomes reveal multiple populations with distinct spatial temporal distributions, including several taxa are previously characterized members. Diverse functional profiles imply high level niche heterogeneity among contrast sole detected AOA representative was cultivated same biofilm. Our metagenome also two cyanase-encoding suggesting ability degrade cyanate, which has shown for representatives strict nitrite oxidizers. demonstrates importance RBCs as model continued investigation environmental factors control activities AOB, AOA,

Language: Английский

Citations

134
Comammox Nitrospira within the Yangtze River continuum: community, biogeography, and ecological drivers DOI Creative Commons
Shufeng Liu, Haiying Wang, Liming Chen

et al.

The ISME Journal, Journal Year: 2020, Volume and Issue: 14(10), P. 2488 - 2504

Published: June 18, 2020

Abstract The recent discovery of comammox Nitrospira as complete nitrifiers has fundamentally renewed perceptions nitrogen cycling in natural and engineered systems, yet little is known about the environmental controls on these newly recognized bacteria. Based improved phylogenetic resolution through successful assembly ten novel genomes (71–96% completeness), we provided first biogeographic patterns for planktonic benthic Yangtze River over a 6030 km continuum. Our study revealed widespread distributions relative abundance this large freshwater system, constituting 30 46% ammonia-oxidizing prokaryotes (AOPs) displaying 30.4- 17.9-fold greater abundances than canonical representatives water sediments, respectively. Comammox contributed more to nitrifier (34–87% AOPs) typical oligotrophic environments with higher pH lower temperature, particularly plateau (clade B), mountain foothill A) areas upper reach. dominant position was replaced by sublineages I/II bacteria from downstream plain due selection, while dissimilarity moderately associated geographic distance. A substantial decrease (83%) occurred immediately Three Gorges Dam, consistent similarly considerable overall sediment bacterial taxa. Together, highlights previously unrecognized dominance major river systems underlines importance revisiting nitrification processes within global environments.

Language: Английский

Citations

124
Does environmental decentralization exacerbate China's carbon emissions? Evidence based on dynamic threshold effect analysis DOI
Qiying Ran, Jinning Zhang, Yu Hao

et al.

The Science of The Total Environment, Journal Year: 2020, Volume and Issue: 721, P. 137656 - 137656

Published: March 3, 2020

Language: Английский

Citations

122
Dominance of comammox Nitrospira in soil nitrification DOI

Jiajie Hu,

Yuxiang Zhao,

Xiangwu Yao

et al.

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 780, P. 146558 - 146558

Published: March 19, 2021

Language: Английский

Citations

100
Comammox Nitrospira Species Dominate in an Efficient Partial Nitrification–Anammox Bioreactor for Treating Ammonium at Low Loadings DOI
Yung-Hsien Shao, Jer‐Horng Wu

Environmental Science & Technology, Journal Year: 2021, Volume and Issue: 55(3), P. 2087 - 2098

Published: Jan. 13, 2021

Bacteria capable of complete ammonia oxidation (comammox) are widespread and contribute to nitrification in wastewater treatment facilities. However, their roles partial nitrification-anaerobic ammonium (anammox) systems remain unclear. In this study, a bench-scale bioreactor with continuous stirring was operated for more than 1000 days limited oxygen supply achieve efficient nitrogen removal (70.1 ± 2.7%) at low loading 35.2 mg-N/L/day. High-throughput amplicon sequencing analysis the comammox monooxygenase subunit A (amoA) gene revealed seven sequence types from two clusters clade Nitrospira. Quantitative polymerase chain reaction analyses suggested that species dominated ammonia-oxidizing community, an abundance as high 89.2 7.9% total prokaryotic amoA copies. Multiple linear regression further substantial contribution Nitrospira bioreactor. The investigation batch experiments consistently showed activities were inhibited by free far severely other microbes. Overall, study provided new insight into ecology under hypoxic conditions comammox-associated nitrification-anammox potential method treating low-strength ammonium-containing wastewater.

Language: Английский

Citations

92
Biogeographic distribution of comammox bacteria in diverse terrestrial habitats DOI
Yao Shi, Yingying Jiang,

Shanyun Wang

et al.

The Science of The Total Environment, Journal Year: 2020, Volume and Issue: 717, P. 137257 - 137257

Published: Feb. 11, 2020

Language: Английский

Citations

83
Distribution and Diversity of Comammox Nitrospira in Coastal Wetlands of China DOI Creative Commons
Dongyao Sun,

Xiufeng Tang,

Mengyue Zhao

et al.

Frontiers in Microbiology, Journal Year: 2020, Volume and Issue: 11

Published: Oct. 6, 2020

Complete ammonia oxidizers (comammox), able to individually oxidize nitrate, are considered play a significant role in the global nitrogen cycle. However, distribution of comammox Nitrospira estuarine tidal flat wetland and environmental drivers affecting their abundance diversity remain unknown. Here, we present large-scale investigation on geographical along wetlands China, where were successfully detected 9 16 sampling sites. The ranged from 4.15 × 105 6.67 106 copies/g, 2.21- 5.44-folds lower than canonical oxidizers: ammonia-oxidizing bacteria (AOB) archaea (AOA). Phylogenetic analysis based alpha subunit monooxygenase encoding gene (amoA) revealed that Clade A, mainly originating upstream river inputs, accounts for more 80% Nitrospira, whereas clade B rarely detected. Comammox dominant OTUs varied within samples, showing pattern. Salinity pH most important wetlands. was further negatively correlated with high nitrite concentrations. Altogether, this study existence, driving factors ecosystems, thus providing insights into ecological niches recently discovered nitrifying consortium contributions nitrification environments.

Language: Английский

Citations

80
Identification, interactions, nitrogen removal pathways and performances of culturable heterotrophic nitrification-aerobic denitrification bacteria from mariculture water by using cell culture and metagenomics DOI
Fei Huang, Luqing Pan, Ziyan He

et al.

The Science of The Total Environment, Journal Year: 2020, Volume and Issue: 732, P. 139268 - 139268

Published: May 8, 2020

Language: Английский

Citations

76
Ammonia-oxidizing archaea and complete ammonia-oxidizing Nitrospira in water treatment systems DOI Creative Commons
Sarah Al-Ajeel, Emilie Spasov,

Laura A. Sauder

et al.

Water Research X, Journal Year: 2022, Volume and Issue: 15, P. 100131 - 100131

Published: March 14, 2022

Nitrification, the oxidation of ammonia to nitrate via nitrite, is important for many engineered water treatment systems. The sequential steps this respiratory process are carried out by distinct microbial guilds, including ammonia-oxidizing bacteria (AOB) and archaea (AOA), nitrite-oxidizing (NOB), newly discovered members genus Nitrospira that conduct complete (comammox). Even though all these nitrifiers have been identified within systems, their relative contributions nitrogen cycling poorly understood. Although AOA contribute nitrification in wastewater plants, they generally outnumbered AOB. In contrast, comammox typically dominate relatively low environments such as drinking treatment, tertiary aquaculture/aquarium filtration. Studies focus on abundance oxidizers may misconstrue actual role nitrifying guilds play a system. Understanding which active useful further optimization systems rely removal. This review highlights known distributions suggests future research directions will help assess identify factors influence activity.

Language: Английский

Citations

60