Wastewater surveillance to infer COVID-19 transmission: A systematic review

The Science of The Total Environment, Год журнала: 2021, Номер 804, С. 150060 - 150060

Опубликована: Сен. 8, 2021

Successful detection of SARS-COV-2 in wastewater suggests the potential utility wastewater-based epidemiology (WBE) for COVID-19 community surveillance. This systematic review aims to assess performance surveillance as early warning system transmission. A search was conducted PubMed, Medline, Embase and WBE Consortium Registry according PRISMA guidelines relevant articles published until 31st July 2021. Relevant data were extracted summarized. Quality each paper assessed using an assessment tool adapted from Bilotta et al.'s environmental science. Of 763 studies identified, 92 distributed across 34 countries shortlisted qualitative synthesis. total 26,197 samples collected between January 2020 May 2021 various locations serving population ranging 321 11,400,000 inhabitants. Overall sample positivity moderate at 29.2% all examined settings with spike (S) gene having maximum rate positive detections nucleocapsid (N) being most targeted. Wastewater signals preceded confirmed cases by up 63 days, 13 reporting before first detected community. At least 50 reported association viral load cases. While cannot replace large-scale diagnostic testing, it can complement clinical providing signs transmission more active public health responses. However, standardized validated methods are required along risk analysis modelling understand dynamics outbreaks.

Язык: Английский

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Targeted wastewater surveillance of SARS-CoV-2 on a university campus for COVID-19 outbreak detection and mitigation

Environmental Research, Год журнала: 2021, Номер 200, С. 111374 - 111374

Опубликована: Май 29, 2021

Язык: Английский

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Wastewater surveillance of SARS-CoV-2 across 40 U.S. states from February to June 2020

Water Research, Год журнала: 2021, Номер 202, С. 117400 - 117400

Опубликована: Июль 2, 2021

Язык: Английский

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Rapid, Large-Scale Wastewater Surveillance and Automated Reporting System Enable Early Detection of Nearly 85% of COVID-19 Cases on a University Campus

mSystems, Год журнала: 2021, Номер 6(4)

Опубликована: Авг. 10, 2021

Wastewater-based surveillance has gained prominence and come to the forefront as a leading indicator of forecasting COVID-19 (coronavirus disease 2019) infection dynamics owing its cost-effectiveness ability inform early public health interventions. A university campus could especially benefit from wastewater surveillance, universities are characterized by largely asymptomatic populations potential hot spots for transmission that necessitate frequent diagnostic testing. In this study, we employed large-scale GIS (geographic information systems)-enabled building-level monitoring system associated with on-campus residences 7,614 individuals. Sixty-eight automated samplers were deployed monitor 239 buildings focus on residential buildings. Time-weighted composite samples collected daily basis analyzed same day. Sample processing was streamlined significantly through automation, reducing turnaround time 20-fold exceeding scale similar programs 10- 100-fold, thereby overcoming one biggest bottlenecks in surveillance. An notification developed alert residents positive sample their residence encourage uptake campus-provided testing at no charge. This system, integrated rest "Return Learn" program University California (UC) San Diego-led diagnosis nearly 85% all cases campus. rates increased 1.9 13× following notifications. Our study shows robust, efficient greatly reduce risk college campuses other high-risk environments reopen. IMPORTANCE epidemiology can be particularly valuable where high-resolution spatial sampling well-controlled context not only provide insight into what affects community well how those inferences extended broader city/county context. present successfully implemented large enabling detection averting outbreaks. The highly reporting enabled dramatic reduction 5 h (sample result time) 96 samples. Furthermore, miniaturization pipeline brought down cost ($13/sample). Taken together, these results show such ameliorate long-term communities they look

Язык: Английский

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Wastewater Surveillance for SARS-CoV-2 on College Campuses: Initial Efforts, Lessons Learned, and Research Needs

International Journal of Environmental Research and Public Health, Год журнала: 2021, Номер 18(9), С. 4455 - 4455

Опубликована: Апрель 22, 2021

Wastewater surveillance for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an emerging approach to help identify risk of a disease (COVID-19) outbreak. This tool can contribute public health at both community (wastewater treatment system) and institutional (e.g., colleges, prisons, nursing homes) scales. paper explores successes, challenges, lessons learned from initial wastewater efforts colleges university systems inform future research, development implementation. We present experiences 25 college in United States that monitored campus SARS-CoV-2 during fall 2020 academic period. describe broad range approaches, findings, resources, impacts these efforts. These institutions size, social political geographies, include private institutions. Our analysis suggests monitoring requires consideration local information needs, sewage infrastructure, resources sampling analysis, dynamics, approaches interpretation communication results, follow-up actions. Most reported learning process experimentation, evaluation, adaptation was key progress. ongoing collaboration among diverse stakeholders including decision-makers, researchers, faculty, facilities staff, students, members.

Язык: Английский

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SARS-CoV-2 Wastewater Surveillance for Public Health Action

Emerging infectious diseases, Год журнала: 2021, Номер 27(9), С. 1 - 8

Опубликована: Авг. 19, 2021

Abstract Wastewater surveillance for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has garnered extensive public attention during the disease pandemic as a proposed complement to existing systems. Over past year, methods detection and quantification of SARS-CoV-2 viral RNA in untreated sewage have advanced, concentrations wastewater been shown correlate with trends reported cases. Despite promise surveillance, these measurements translate into useful health tools, bridging communication knowledge gaps between researchers responders is needed. We describe key uses, barriers, applicability supporting decisions actions, including establishing ethics consideration monitoring. Although assess community infections not new idea, might be initiating event make this emerging tool sustainable nationwide system, provided that barriers are addressed.

Язык: Английский

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Metrics to relate COVID-19 wastewater data to clinical testing dynamics

Water Research, Год журнала: 2022, Номер 212, С. 118070 - 118070

Опубликована: Янв. 14, 2022

Wastewater surveillance has emerged as a useful tool in the public health response to COVID-19 pandemic. While wastewater been applied at various scales monitor population-level dynamics, there is need for quantitative metrics interpret data context of trends. 24-hour composite samples were collected from March 2020 through May 2021 Massachusetts treatment plant and SARS-CoV-2 RNA concentrations measured using RT-qPCR. The relationship between copy numbers gene fragments clinical cases deaths varies over time. We demonstrate utility three new changes epidemiology: (1) ratio (WC ratio), (2) time lag reporting, (3) transfer function case curves. WC increases after key events, providing insight into balance disease spread response. Time analysis showed that preceded clinically reported first wave pandemic but did not serve leading indicator second wave, likely due increased testing capacity, which allows more timely detection reporting. These could help further integrate future pandemics.

Язык: Английский

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Comparison of RT-qPCR and RT-dPCR Platforms for the Trace Detection of SARS-CoV-2 RNA in Wastewater

ACS ES&T Water, Год журнала: 2022, Номер 2(11), С. 1871 - 1880

Опубликована: Янв. 28, 2022

We compared reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and RT digital PCR (RT-dPCR) platforms for the trace detection of SARS-CoV-2 RNA in low-prevalence COVID-19 locations Queensland, Australia, using CDC N1 N2 assays. The assay limit (ALOD), inhibition rates, performance characteristics each assay, along with positivity rates RT-qPCR RT-dPCR platforms, were evaluated by seeding known concentrations exogenous wastewater. ALODs approximately 2-5 times lower than those RT-qPCR. During sample processing, endogenous (n = 96) 24) wastewater samples separated, was extracted from both eluates pellets (solids). platform demonstrated a rate significantly greater that assays eluate (N1, p 0.0029; N2, 0.0003) pellet 0.0015; 0.0067) samples. results also indicated analysis wastewater, including may further increase sensitivity RT-dPCR.

Язык: Английский

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A wastewater-based epidemic model for SARS-CoV-2 with application to three Canadian cities

Epidemics, Год журнала: 2022, Номер 39, С. 100560 - 100560

Опубликована: Апрель 9, 2022

The COVID-19 pandemic has stimulated wastewater-based surveillance, allowing public health to track the epidemic by monitoring concentration of genetic fingerprints SARS-CoV-2 shed in wastewater infected individuals. Wastewater-based surveillance for is still its infancy. In particular, quantitative link between clinical cases observed through traditional and signals from viral concentrations developing hampers interpretation data actionable public-health decisions. We present a modelling framework that includes both transmission at population level fate RNA particles sewage system after faecal shedding persons population. Using our mechanistic representation combined clinical/wastewater system, we perform exploratory simulations quantify effect effectiveness, interventions vaccination on discordance signals. also apply model three Canadian cities provide wastewater-informed estimates actual prevalence, effective reproduction number incidence forecasts. find paired with this model, can complement supporting estimation key epidemiological metrics hence better triangulate state an using alternative source.

Язык: Английский

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Correlation between SARS-CoV-2 RNA concentration in wastewater and COVID-19 cases in community: A systematic review and meta-analysis

Journal of Hazardous Materials, Год журнала: 2022, Номер 441, С. 129848 - 129848

Опубликована: Авг. 28, 2022

Язык: Английский

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