Water Research, Год журнала: 2023, Номер 241, С. 120116 - 120116
Опубликована: Май 23, 2023
Язык: Английский
American Journal of Epidemiology, Год журнала: 2022, Номер 192(2), С. 305 - 322
Опубликована: Окт. 13, 2022
Abstract Wastewater surveillance for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been shown to be a valuable source of information regarding SARS-CoV-2 transmission and disease 2019 (COVID-19) cases. Although the method used several decades track other infectious diseases, there not comprehensive review outlining all pathogens that have surveilled through wastewater. Herein we identify diseases previously studied via wastewater prior COVID-19 pandemic. Infectious were identified in 100 studies across 38 countries, as themes how measures linked. Twenty-five separate pathogen families included studies, with majority examining from family Picornaviridae, including polio nonpolio enteroviruses. Most did link what was found transmission. Among those did, value reported varied by study. should considered potential public health tool many diseases. can improved incorporating at population-level incidence hospitalizations.
Язык: Английский
Процитировано
115
The Science of The Total Environment, Год журнала: 2022, Номер 837, С. 155663 - 155663
Опубликована: Май 4, 2022
Язык: Английский
Процитировано
80
The Science of The Total Environment, Год журнала: 2023, Номер 878, С. 162953 - 162953
Опубликована: Март 21, 2023
Язык: Английский
Процитировано
63
Environment International, Год журнала: 2024, Номер 183, С. 108438 - 108438
Опубликована: Янв. 1, 2024
The increased frequency of human infectious disease outbreaks caused by RNA viruses worldwide in recent years calls for enhanced public health surveillance better future preparedness. Wastewater-based epidemiology (WBE) is emerging as a valuable epidemiological tool providing timely population-wide prevention and response complementary to the current clinical system. Here, we compared analytical performance practical applications between predominant molecular detection methods RT-qPCR RT-ddPCR on SARS-CoV-2 wastewater surveillance. When pure viral was tested, exhibited superior quantification accuracy at higher concentration levels achieved more sensitive with reduced variation low levels. Furthermore, consistently demonstrated robust accurate measurement either background matrix or presence mismatches target regions consensus assay. Additionally, detecting mock variant samples, found that outperformed virus genotyping targeting specific loci signature mutations allele-specific (AS) assays, especially allele frequencies concentrations, which possibility low-prevalence population. Our study provides insights method selection WBE outbreaks.
Язык: Английский
Процитировано
18
Water Research, Год журнала: 2022, Номер 213, С. 118132 - 118132
Опубликована: Фев. 3, 2022
Effective wastewater surveillance of SARS-CoV-2 RNA requires the rigorous characterization limit detection resulting from entire sampling process - (PLOD). Yet to date, no studies have gone beyond quantifying assay (ALOD) for RT-qPCR or RT-dPCR assays. While ALOD is lowest number gene copies (GC) associated with a 95% probability in single PCR reaction, PLOD represents sensitivity method after considering efficiency all processing steps (e.g., sample handling, concentration, nucleic acid extraction, and assays) determine GC matrix specific detection. The primary objective this study was estimate combination concentration extraction six assays: five assays (US CDC N1 N2, China N ORF1ab (CCDC CCDC ORF1ab), E_Sarbeco RT-qPCR, one RT-dPCR) using two models (exponential survival cumulative Gaussian). An adsorption (AE) (i.e., virus on membrane membrane) used concentrate gamma-irradiated seeded into 36 samples. Overall, US had ALODs (< 10 GC/reaction) PLODs (<3,954 GC/50 mL; detection) regardless seeding level model used. Nevertheless, consistent amplification rates decreased when levels were < 2.32 × 103 mL even Consequently, concentrations are expected be low, it may necessary improve positive by analyzing additional field RT-PCR replicates. To best our knowledge, first assess provides important insights analytical limitations trace wastewater.
Язык: Английский
Процитировано
65
Microorganisms, Год журнала: 2022, Номер 10(6), С. 1193 - 1193
Опубликована: Июнь 10, 2022
COVID-19 emerged in late 2019 China and quickly spread across the globe, causing over 521 million cases of infection 6.26 deaths to date. After 2 years, numerous advances have been made. First all, preventive vaccine, which has implemented record time, is effective more than 95% cases. Additionally, diagnostic field, there are molecular antigenic kits that equipped with high sensitivity specificity. Real Time-PCR-based assays for detection viral RNA currently considered gold-standard method SARS-CoV-2 diagnosis can be used efficiently on pooled nasopharyngeal, or oropharyngeal samples widespread screening. Moreover, additional, advanced methods such as droplet-digital PCR (ddPCR), clustered regularly interspaced short palindromic repeats (CRISPR) next-generation sequencing (NGS), under development detect RNA. However, number subjects infected continuously increases globally, health care systems being placed increased stress. Thus, clinical laboratory plays an important role, helping select especially asymptomatic individuals who actively carrying live replicating virus, fast non-invasive technologies. Recent strategies, other methods, adopted either antigens, i.e., antigen-based immunoassays, human anti-SARS-CoV-2 antibodies, antibody-based nasal swabs, well blood saliva samples. role mucosal sIgAs, essential control viruses entering body through surfaces, remains elucidated, particular immune response counteracting infection, primarily at site(s) virus entry appears promising.
Язык: Английский
Процитировано
53
The Science of The Total Environment, Год журнала: 2022, Номер 833, С. 155059 - 155059
Опубликована: Апрель 6, 2022
Язык: Английский
Процитировано
48
The Science of The Total Environment, Год журнала: 2022, Номер 857, С. 159401 - 159401
Опубликована: Окт. 12, 2022
Understanding the persistence of SARS-CoV-2 biomarkers in wastewater should guide wastewater-based epidemiology users selecting best RNA for reliable detection virus during current and future waves pandemic. In present study, endogenous were assessed one month six different pepper mild mottle (PMMoV) at three temperatures (4, 12 20 °C) sample. All consistently detected 6 days 4° differences signal among mostly observed °C with N being globally more persistent than RdRP, E ORF1ab ones. further decreased a temperature dependent manner. At °C, biomarker losses 1-log
Язык: Английский
Процитировано
41
The Science of The Total Environment, Год журнала: 2022, Номер 853, С. 158659 - 158659
Опубликована: Сен. 9, 2022
Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are continuously emerging, highlighting the importance regular surveillance SARS-CoV-2 and other epidemiologically significant pathogenic viruses in current context. Reverse transcription-quantitative PCR (RT-qPCR) is expensive, time-consuming, labor-intensive, requires a large reagent volume, only tests few targets single run. High-throughput qPCR (HT-qPCR) utilizing Biomark HD system (Fluidigm) can be used as an alternative. This study applied HT-qPCR to simultaneously detect SARS-CoV-2, nucleotide substituted RNA, wastewater. Wastewater samples were collected from disease 2019 (COVID-19) quarantine facility between October 2020 February 2021 (n = 4) combined separated sewer lines wastewater treatment plant (WWTP) Yokkaichi, Mie Prefecture, Japan, March August 23 each). The analyzed by using five nine spike gene substitution-specific, viruses, three process control assays. All tested positive for substitutions N501Y S69-70 del (Alpha variant) detected December sample, coinciding with first clinical case Japan. Only WWTP when assay, whereas more than eight all assays, indicating that multiple assays increases likelihood detection. substitution L452R (Delta was Prefecture April 2021, but detection Delta variant patients had not been reported until May 2021. Aichi virus 1 norovirus GII prevalent samples. demonstrated may most time- cost-efficient method tracking COVID-19 broadly monitoring community health.
Язык: Английский
Процитировано
40
Water, Год журнала: 2023, Номер 15(6), С. 1018 - 1018
Опубликована: Март 7, 2023
The emergence of new variants SARS-CoV-2 associated with varying infectivity, pathogenicity, diagnosis, and effectiveness against treatments challenged the overall management COVID-19 pandemic. Wastewater surveillance (WWS), i.e., monitoring infections in communities through detecting viruses wastewater, was applied to track spread globally. However, there is a lack comprehensive understanding use WWS for variants. Here we systematically reviewed published articles reporting different wastewater by following PRISMA guidelines provided current state art this study area. A total 80 studies were found that reported until November 2022. Most these (66 out 80, 82.5%) conducted Europe North America, resource-rich countries. There high variation sampling strategy around world, composite (50/66 studies, 76%) as primary method In contrast, grab more common (8/14 57%) resource-limited Among detection methods, reverse transcriptase polymerase chain reaction (RT-PCR)-based sequencing quantitative RT-PCR commonly used wastewater. variants, B1.1.7 (Alpha) variant appeared earlier pandemic most (48/80 studies), followed B.1.617.2 (Delta), B.1.351 (Beta), P.1 (Gamma), others All same pattern clinical within timeline, demonstrating tracked all timely way when emerged. Thus, may be utilized identify presence or absence follow development transmission existing emerging Routine powerful infectious disease tool implemented
Язык: Английский
Процитировано
40