The Science of The Total Environment, Год журнала: 2022, Номер 838, С. 156535 - 156535
Опубликована: Июнь 7, 2022
Язык: Английский
Water Research, Год журнала: 2022, Номер 212, С. 118070 - 118070
Опубликована: Янв. 14, 2022
Wastewater surveillance has emerged as a useful tool in the public health response to COVID-19 pandemic. While wastewater been applied at various scales monitor population-level dynamics, there is need for quantitative metrics interpret data context of trends. 24-hour composite samples were collected from March 2020 through May 2021 Massachusetts treatment plant and SARS-CoV-2 RNA concentrations measured using RT-qPCR. The relationship between copy numbers gene fragments clinical cases deaths varies over time. We demonstrate utility three new changes epidemiology: (1) ratio (WC ratio), (2) time lag reporting, (3) transfer function case curves. WC increases after key events, providing insight into balance disease spread response. Time analysis showed that preceded clinically reported first wave pandemic but did not serve leading indicator second wave, likely due increased testing capacity, which allows more timely detection reporting. These could help further integrate future pandemics.
Язык: Английский
Процитировано
103
The Science of The Total Environment, Год журнала: 2022, Номер 820, С. 153290 - 153290
Опубликована: Янв. 20, 2022
Язык: Английский
Процитировано
89
ACS ES&T Water, Год журнала: 2022, Номер 2(11), С. 1992 - 2003
Опубликована: Май 26, 2022
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in wastewater has been used to track community infections of coronavirus disease-2019 (COVID-19), providing critical information for public health interventions. Since levels are dependent upon human inputs, we hypothesize that tracking can be improved by normalizing concentrations against indicators waste [Pepper Mild Mottle Virus (PMMoV), β-2 Microglobulin (B2M), and fecal coliform]. In this study, analyzed SARS-CoV-2 from two sewersheds different scales: a University campus treatment plant. Wastewater data were combined with complementary COVID-19 case evaluate the efficiency surveillance forecasting new cases and, larger scale, hospitalizations. Results show normalization PMMoV B2M resulted correlations using volcano second generation (V2G)-qPCR chemistry (rs = 0.69 without normalization, rs 0.73 normalization). Mixed results obtained samples collected at scale. Overall benefits measures depend qPCR improves smaller sewershed We recommend further studies efficacy additional targets.
Язык: Английский
Процитировано
82
The Science of The Total Environment, Год журнала: 2023, Номер 892, С. 164495 - 164495
Опубликована: Май 26, 2023
Wastewater-based surveillance can be a valuable tool to monitor viral circulation and serve as an early warning system. For respiratory viruses that share similar clinical symptoms, namely SARS-CoV-2, influenza, syncytial virus (RSV), identification in wastewater may allow differentiation between seasonal outbreaks COVID-19 peaks. In this study, these well standard indicators of fecal contamination, weekly sampling campaign was carried out for 15 months (from September 2021 November 2022) two treatment plants the entire population Barcelona (Spain). Samples were concentrated by aluminum hydroxide adsorption-precipitation method then analyzed RNA extraction RT-qPCR. All samples positive while positivity rates influenza RSV significantly lower (10.65 % A (IAV), 0.82 B (IBV), 37.70 RSV-A 34.43 RSV-B). Gene copy concentrations SARS-CoV-2 often approximately 1 2 logarithmic units higher compared other viruses. Clear peaks IAV H3:N2 February March 2022 winter observed, which matched chronological incidence infections recorded Catalan Government database. conclusion, data obtained from provided new information on abundance area correlated favorably with data.
Язык: Английский
Процитировано
66
The Science of The Total Environment, Год журнала: 2023, Номер 878, С. 162953 - 162953
Опубликована: Март 21, 2023
Язык: Английский
Процитировано
63
Environmental Science & Technology, Год журнала: 2023, Номер 57(4), С. 1755 - 1763
Опубликована: Янв. 19, 2023
Candida auris is an opportunistic fungal pathogen and emerging global public health threat, given its high mortality among infected individuals, antifungal resistance, persistence in healthcare environments. This study explored the applicability of wastewater surveillance for C. a metropolitan area with reported outbreaks across multiple facilities. Influent or primary effluent samples were collected over 10 weeks from seven sewersheds Southern Nevada. Pelleted solids analyzed using adapted quantitative polymerase chain reaction (qPCR) assay targeting ITS2 region genome. Positive detection was observed 72 91 (79%), higher frequencies serving facilities involved outbreak (94 vs 20% sample positivity). concentrations ranged 2.8 to 5.7 log10 gene copies per liter (gc/L), clarification achieved average log reduction value (LRV) 1.24 ± 0.34. Presumptive negative surface water controls non-detect. These results demonstrate that may assist tracking spread serve as early warning tool action. findings provide foundation future application wastewater-based epidemiology (WBE) community- facility-level other consequence, healthcare-associated infectious agents.
Язык: Английский
Процитировано
55
Microbiology Spectrum, Год журнала: 2021, Номер 9(2)
Опубликована: Окт. 7, 2021
A wastewater surveillance program targeting a university residence hall was implemented during the spring semester 2021 as proactive measure to avoid an outbreak of COVID-19 on campus. Over period 7 weeks from early February through late March 2021, originating collected grab samples 3 times per week. During this time, there no detection SARS-CoV-2 by reverse transcriptase quantitative PCR (RT-qPCR) in stream. Aiming obtain sample more representative community, decision made use passive samplers beginning onwards. Adopting Moore swab approach, detected just 2 days after were deployed. These also tested positive for B.1.1.7 (Alpha) variant concern (VOC) using RT-qPCR. The result triggered public health case-finding response, including mobile testing unit deployed following day, with nearly 200 students and staff, which identified two laboratory-confirmed cases Alpha COVID-19. individuals relocated separate quarantine facility, averting Aggregating clinical data, campus has yielded first estimates fecal shedding rates VOC nonclinical setting. IMPORTANCE Among adopters monitoring have been colleges universities throughout North America, many whom are approach monitor congregate living facilities evidence infection integral component screening programs. Yet, while numerous examples where among community members, few response that may averted actual outbreak. This report details wastewater-testing when mounting globally over emergence variants concern, reported be transmissible than wild-type Wuhan strain. In communication, we present clear example how resulted actionable responses administration health,
Язык: Английский
Процитировано
79
Water, Год журнала: 2021, Номер 13(20), С. 2897 - 2897
Опубликована: Окт. 15, 2021
Wastewater-based epidemiology (WBE) is an approach that can be used to estimate COVID-19 prevalence in the population by detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA wastewater. As WBE uses pooled samples from study population, it inexpensive and non-invasive mass surveillance method compared individual testing. Thus, offers a good complement low- middle-income countries (LMICs) facing high costs of testing or social stigmatization, has huge potential monitor SARS-CoV-2 its variants curb global pandemic. The aim this review systematize current evidence about application infection LMICs, as well future potential. Among other parameters, size contributing fecal input wastewater important parameter for estimation. It easier back-calculate community with centralized systems, because there more accurate estimates catchment. However, management systems are often low quality (or even non-existent) which raises major concern ability implement approach. possible mobilize approach, if large areas divided into sub-areas, corresponding existing systems. In addition, strong coordination between stakeholders required estimating respective Nevertheless, further international efforts should leveraged strengthen sanitation infrastructures using lessons gathered pandemic prepared pandemics.
Язык: Английский
Процитировано
63
The Science of The Total Environment, Год журнала: 2022, Номер 824, С. 153816 - 153816
Опубликована: Фев. 11, 2022
The applicability of wastewater-based epidemiology (WBE) has been extensively studied throughout the world with remarkable findings. This study reports presence and reduction severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at two wastewater treatment plants (WWTPs) Nepal, along river water, hospital (HWW), from sewer lines collected between July 2020 February 2021. SARS-CoV-2 RNA was detected in 50%, 54%, 100%, 100% water samples WWTPs, hospitals, lines, respectively, by least one four quantitative PCR assays tested (CDC-N1, CDC-N2, NIID_2019-nCOV_N, N_Sarbeco). CDC-N2 assay highest number raw influent both WWTPs. concentration observed for an sample WWTP A (5.5 ± 1.0 log
Язык: Английский
Процитировано
63
The Science of The Total Environment, Год журнала: 2021, Номер 809, С. 151169 - 151169
Опубликована: Окт. 25, 2021
Wastewater surveillance for SARS-CoV-2 RNA has been a successful indicator of COVID-19 outbreaks in populations prior to clinical testing. However, this mostly conducted high-income countries, which means there is dearth performance investigations low- and middle-income countries with different socio-economic settings. This study evaluated the applicability monitoring wastewater (n = 132) inform infection city Bangkok, Thailand using CDC N1 N2 RT-qPCR assays. influents 112) effluents 20) were collected from 19 centralized treatment plants (WWTPs) comprising four large, medium, 11 small WWTPs during seven sampling events January April 2021 third resurgence that was officially declared 2021. The assay showed higher detection rates lower Ct values than N2. first detected at event when new reported cases low. Increased positive preceded an increase number newly increased over time incidence. (both viral loads) strongest correlation daily 22-24 days lag (Spearman's Rho 0.85-1.00). Large (serving 432,000-580,000 population) exhibited similar trends loads those all WWTPs, emphasizing routine large could provide sufficient information city-scale dynamics. Higher frequency fewer sites, i.e., representative therefore suggested especially subsiding period outbreak indicate prevalence infection, acting as early warning resurgence.
Язык: Английский
Процитировано
59