Systematic SARS-CoV-2 S-gene sequencing in wastewater samples enables early lineage detection and uncovers rare mutations in Portugal DOI Creative Commons
Ana C. Reis, Daniela Pinto, Sílvia Monteiro

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 921, С. 170961 - 170961

Опубликована: Фев. 15, 2024

As the COVID-19 pandemic reached its peak, many countries implemented genomic surveillance systems to track evolution and transmission of SARS-CoV-2. Transition from endemic phase prioritized alternative testing strategies maintain effective epidemic at population level, with less intensive sequencing efforts. One such promising approach was Wastewater-Based Surveillance (WBS), which offers non-invasive, cost-effective means for analysing virus trends sewershed level. From 2020 onwards, wastewater has been recognized as an instrumental source information public health, national international authorities exploring options implement increasingly relying on WBS early warning potential pathogen outbreaks. In Portugal, several pioneer projects joined academia, water utilities Public Administration around WBS. To validate strategy, it is crucial collect long term performance data. this work, we present one year systematic SARS-CoV-2 in representing 35 % mainland population. We employed two complementary methods lineage determination - allelic discrimination by RT-PCR S-gene sequencing. This combination allowed us monitor variant near-real-time identify low-frequency mutations. Over course year-long study, spanning May 2022 April 2023, successfully tracked dominant Omicron sub-lineages, their progression evolution, aligned concurrent clinical Our results underscore effectiveness a tracking system variants, ability unveil mutations undetected via massive samples demonstrating uncover new detect rare genetic variants. findings emphasize that knowledge diversity level can be extended far beyond routine genotyping.

Язык: Английский

Monitoring influenza and respiratory syncytial virus in wastewater. Beyond COVID-19 DOI Creative Commons
Daniel Toribio-Avedillo, Clara Gómez-Gómez, Laura Sala‐Comorera

и другие.

The Science of The Total Environment, Год журнала: 2023, Номер 892, С. 164495 - 164495

Опубликована: Май 26, 2023

Wastewater-based surveillance can be a valuable tool to monitor viral circulation and serve as an early warning system. For respiratory viruses that share similar clinical symptoms, namely SARS-CoV-2, influenza, syncytial virus (RSV), identification in wastewater may allow differentiation between seasonal outbreaks COVID-19 peaks. In this study, these well standard indicators of fecal contamination, weekly sampling campaign was carried out for 15 months (from September 2021 November 2022) two treatment plants the entire population Barcelona (Spain). Samples were concentrated by aluminum hydroxide adsorption-precipitation method then analyzed RNA extraction RT-qPCR. All samples positive while positivity rates influenza RSV significantly lower (10.65 % A (IAV), 0.82 B (IBV), 37.70 RSV-A 34.43 RSV-B). Gene copy concentrations SARS-CoV-2 often approximately 1 2 logarithmic units higher compared other viruses. Clear peaks IAV H3:N2 February March 2022 winter observed, which matched chronological incidence infections recorded Catalan Government database. conclusion, data obtained from provided new information on abundance area correlated favorably with data.

Язык: Английский

Процитировано

67
Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling DOI Open Access
Stéfano Ciannella, Cristina González-Fernández, Jenifer Gómez‐Pastora

и другие.

The Science of The Total Environment, Год журнала: 2023, Номер 878, С. 162953 - 162953

Опубликована: Март 21, 2023

Язык: Английский

Процитировано

63
Key considerations for pathogen surveillance in wastewater DOI Creative Commons
Ananda Tiwari, Elena Radu, Norbert Kreuzinger

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 945, С. 173862 - 173862

Опубликована: Июнь 12, 2024

Wastewater surveillance (WWS) has received significant attention as a rapid, sensitive, and cost-effective tool for monitoring various pathogens in community. WWS is employed to assess the spatial temporal trends of diseases identify their early appearances reappearances, well detect novel mutated variants. However, shedding rates vary significantly depending on factors such disease severity, physiology affected individuals, characteristics pathogen. Furthermore, may exhibit differential fate decay kinetics sewerage system. Variable affect detection wastewater. This influence interpretation results conclusions studies. When selecting pathogen WWS, it essential consider it's specific characteristics. If data are not readily available, fate, decay, should be assessed before conducting surveillance. Alternatively, these can compared those similar which available.

Язык: Английский

Процитировано

19
Clinical and laboratory diagnosis of monkeypox (mpox): Current status and future directions DOI Creative Commons
Severino Jefferson Ribeiro da Silva, Alain Kohl, Lindomar Pena

и другие.

iScience, Год журнала: 2023, Номер 26(6), С. 106759 - 106759

Опубликована: Апрель 28, 2023

The emergence and rapid spread of the monkeypox virus (MPXV) to non-endemic countries has brought this once obscure pathogen forefront global public health. Given range conditions that cause similar skin lesions, because clinical manifestation may often be atypical in current mpox outbreak, it can challenging diagnose patients based on signs symptoms. With perspective mind, laboratory-based diagnosis assumes a critical role for management, along with implementation countermeasures. Here, we review features reported patients, available laboratory tests diagnosis, discuss principles, advances, advantages, drawbacks each assay. We also highlight diagnostic platforms potential guide ongoing response, particularly those increase capacity low- middle-income countries. outlook evolving research area, hope provide resource community inspire more development alternatives applications future health crises.

Язык: Английский

Процитировано

36
Antibiotic resistance genes in bacteriophages from wastewater treatment plant and hospital wastewaters DOI Creative Commons
Joana Pires, Ricardo Santos, Sílvia Monteiro

и другие.

The Science of The Total Environment, Год журнала: 2023, Номер 892, С. 164708 - 164708

Опубликована: Июнь 12, 2023

Antibiotic resistant bacteria (ARB) are a major health risk caused particularly by anthropogenic activities. Acquisition of antibiotic resistances is known to have happened before the discovery antibiotics and can occur through different routes. Bacteriophages thought an important contribution dissemination resistance genes (ARGs) in environment. In this study, seven ARGs (blaTEM, blaSHV, blaCTX-M, blaCMY, mecA, vanA, mcr-1) were investigated, bacteriophage fraction, raw urban hospital wastewaters. The quantified 58 wastewater samples collected at five WWTPs (n = 38) hospitals 20). All detected phage DNA with bla found higher frequency. On other hand, mecA mcr-1 least frequently genes. Concentrations varied between 102 copies/L 106 copies/L. gene coding for colistin (mcr-1), last-resort treatment multidrug-resistant Gram-negative infections, was identified wastewaters positivity rates 19 % 10 %, respectively. patterns wastewaters, within WWTP. This study suggests that phages reservoirs ARGs, (with emphasis on vancomycin) fraction already widely widespread environment potential large implications public health.

Язык: Английский

Процитировано

34
COVID-19 monitoring with sparse sampling of sewered and non-sewered wastewater in urban and rural communities DOI Creative Commons
Dhammika Leshan Wannigama, Mohan Amarasiri, Parichart Hongsing

и другие.

iScience, Год журнала: 2023, Номер 26(7), С. 107019 - 107019

Опубликована: Июнь 9, 2023

Язык: Английский

Процитировано

27
Wastewater surveillance for viral pathogens: A tool for public health DOI Creative Commons
Matheus Carmo dos Santos,

Ana Clara Cerqueira Silva,

Carine dos Reis Teixeira

и другие.

Heliyon, Год журнала: 2024, Номер 10(13), С. e33873 - e33873

Опубликована: Июнь 29, 2024

A focus on water quality has intensified globally, considering its critical role in sustaining life and ecosystems. Wastewater, reflecting societal development, profoundly impacts public health. Wastewater-based epidemiology (WBE) emerged as a surveillance tool for detecting outbreaks early, monitoring infectious disease trends, providing real-time insights, particularly vulnerable communities. WBE aids tracking pathogens, including viruses, sewage, offering comprehensive understanding of community health lifestyle habits. With the rise global COVID-19 cases, gained prominence, aiding SARS-CoV-2 levels worldwide. Despite advancements treatment, poorly treated wastewater discharge remains threat, amplifying spread water-, sanitation-, hygiene (WaSH)-related diseases. WBE, serving complementary surveillance, is pivotal community-level viral infections. However, there untapped potential to expand surveillance. This review emphasizes importance link between health, highlighting need further integration into management.

Язык: Английский

Процитировано

14
Detection of dengue virus and chikungunya virus in wastewater in Portugal: an exploratory surveillance study DOI Creative Commons
Sílvia Monteiro,

Raquel Pimenta,

Filipa Nunes

и другие.

The Lancet Microbe, Год журнала: 2024, Номер unknown, С. 100911 - 100911

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

10
Environmental Surveillance of Vector-Borne Diseases in a Non-Sewered System: A Case Study in Mozambique DOI
Sílvia Monteiro, Filipa Nunes, Michaque Dosse

и другие.

Environmental Science & Technology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 14, 2025

Arboviral diseases pose major economic and social threats in less economically developed countries (LEDCs), where monitoring is challenging, especially rapidly growing cities with informal settlements. In this study, we aimed to explore environmental surveillance (ES) a non-sewered setting as complement syndromic Maputo, Mozambique. Water samples were collected from nine points along the Infulene River (n = 66) Mozambique February September 2023. The presence of arboviruses (Dengue (DENV), Chikungunya (CHIKV), West Nile (WNV), Usutu (USUV) virus) was determined by RT-qPCR. For specific detection CHIKV, two RT-qPCR assays used: Nsp1, targeting non-structural protein 1 gene (nsP1) E1, E1 envelope (E1). DENV detected 82% (54/66) samples, median viral RNA load 2.7 × 10-2 (2.2 105 copies/L (cp/L)), while CHIKV detectable 98% (65/66) 4.8 cp/L) for nsP1 8.0 (4.8 cp/L), USUV 6% (4/66) at 4.1 10-7 (0 cP/L), positive varying between 1.8 10-3 (7.1 102 4.95 (2.1 103 cp/L). WNV not throughout study. prevalence concentration varied across sampling dates. Our study demonstrated potential ES tool assessing circulation Mozambique, sewered system unavailable. Consequently, could be expanded polio include other targets LEDCs.

Язык: Английский

Процитировано

1
An opinion on Wastewater-Based Epidemiological Monitoring (WBEM) with Clinical Diagnostic Test (CDT) for detecting high-prevalence areas of community COVID-19 infections DOI Creative Commons
Md. Aminul Islam,

Foysal Hossen,

Arifur Rahman

и другие.

Current Opinion in Environmental Science & Health, Год журнала: 2022, Номер 31, С. 100396 - 100396

Опубликована: Окт. 6, 2022

Wastewater-Based Epidemiological Monitoring (WBEM) is an efficient surveillance tool during the COVID-19 pandemic as it meets all requirements of a complete monitoring system including early warning, tracking current trend, prevalence disease, detection genetic diversity well asthe up-surging SARS-CoV-2 new variants with mutations from wastewater samples. Subsequently, Clinical Diagnostic Test widely acknowledged global gold standard method for disease monitoring, despite several drawbacks such high diagnosis cost, reporting bias, and difficulty asymptomatic patients (silent spreaders infection who manifest nosymptoms disease). In this reviewand opinion-based study, we first propose combined approach) detecting in communities using clinical sample testing, which may be feasible effective emerging public health long-term nationwide system. The viral concentrations samples can used indicatorsto monitor ongoing trends, predict carriers, detect hotspot areas, while sampleshelp mostlysymptomaticindividuals isolating positive cases validate WBEM protocol mass vaccination booster doses COVID-19.

Язык: Английский

Процитировано

37