Analytical and Bioanalytical Chemistry,
Год журнала:
2024,
Номер
416(5), С. 1149 - 1164
Опубликована: Янв. 4, 2024
Abstract
The
conditionally
essential
very-long-chain
polyunsaturated
fatty
acids
(VLC-PUFAs),
such
as
eicosapentaenoic
acid
(EPA,
C20:5
n-3),
play
a
vital
role
in
human
nutrition.
Their
biological
activity
is
thereby
greatly
influenced
by
the
distinct
glycerolipid
molecule
that
they
are
esterified
to.
Here,
microalgae
differ
from
conventional
source,
fish
oil,
both
quantity
and
distribution
of
VLC-PUFAs
among
glycerolipidome.
Therefore,
aim
this
study
was
to
develop
fast
reliable
one-dimensional
high-performance
thin-layer
chromatography
(HPTLC)-based
method
allows
separation
quantification
main
microalgal
classes
(e.g.,
monogalactosyldiacylglycerol
(MGDG),
sulfoquinovosyl
diacylglycerol
(SQDG),
phosphatidylglycerol
(PG)),
well
subsequent
analysis
their
respective
via
gas
(GC)
coupled
mass
spectrometry
(MS).
Following
optimization,
validation
carried
out
for
13
different
lipid
classes,
based
on
International
Conference
Harmonization
(ICH)
guidelines.
In
HPTLC,
linearity
effective
between
100
2100
ng,
with
limit
62.99
90.09
ng
depending
class,
strong
correlation
coefficients
(
R
2
>
0.995).
recovery
varied
93.17
108.12%,
while
inter-day
precision
measurements
showed
variation
less
than
8.85%,
close
detection.
Applying
crude
extracts
four
EPA
producing
commercial
interest,
content
assessed
together
FA
band
elution.
results
described
precise
accurate
HPTLC
offers
possibility
be
used
routinely
follow
variations
class
levels
throughout
strain
screening,
cultivation,
or
bioprocessing.
Thus,
additional
quantitative
analytical
information
complex
lipidome
can
obtained,
especially
n-3
n-6
enriched
fractions.
Graphical
Biology,
Год журнала:
2022,
Номер
11(8), С. 1146 - 1146
Опубликована: Июль 29, 2022
Microalgae
have
demonstrated
a
large
potential
in
biotechnology
as
source
of
various
macromolecules
(proteins,
carbohydrates,
and
lipids)
high-added
value
products
(pigments,
poly-unsaturated
fatty
acids,
peptides,
exo-polysaccharides,
etc.).
The
production
biomass
at
scale
becomes
more
economically
feasible
when
it
is
part
biorefinery
designed
within
the
circular
economy
concept.
Thus,
aim
this
critical
review
to
highlight
discuss
challenges
future
trends
related
multi-product
microalgae-based
biorefineries,
including
both
phototrophic
mixotrophic
cultures
treating
wastewater
recovery
valuable
low-value
(biofertilizers
biostimulants).
therapeutic
properties
some
microalgae-bioactive
compounds
are
also
discussed.
Novel
such
screening
species
for
antimicrobial
compounds,
bioplastics
using
wastewater,
strategy,
need
Life
Cycle
Assessment
studies
(LCA)
suggested
research
lines.
Applied Sciences,
Год журнала:
2023,
Номер
13(21), С. 12088 - 12088
Опубликована: Ноя. 6, 2023
The
structurally
diverse
bioactive
compounds
found
in
marine
organisms
represent
valuable
resources
for
the
food
and
pharmaceutical
industries.
ecosystem
encompasses
over
half
of
world’s
biota,
providing
an
extensive
range
that
can
be
extracted
from
various
life
forms,
including
microorganisms
(such
as
bacteria,
cyanobacteria,
actinobacteria),
algae
(both
macroalgae
microalgae),
invertebrates
(including
sponges,
mollusks,
echinoderms,
crustaceans),
and,
most
importantly,
fish.
Many
these
thrive
extreme
environments,
leading
to
production
complex
molecules
with
unique
biological
functions.
Consequently,
biomolecules,
such
lipids
(especially
polyunsaturated
fatty
acids),
proteins/peptides,
polysaccharides,
carotenoids,
phenolics,
saponins,
exhibit
a
wide
properties
serve
components
nutraceuticals
functional
foods.
Nevertheless,
biomolecules
are
susceptible
oxidation
degradation;
encapsulation-based
technologies
tend
preserve
them
increase
their
bioavailability
These
demonstrate
activities,
antioxidant,
anticancer,
antithrombotic,
anticoagulant,
anti-inflammatory,
antiproliferative,
antidiabetic,
antimicrobial,
cardioprotective
effects,
making
promising
candidates
applications
industry.
Despite
numerous
health
benefits,
have
remained
underutilized,
not
only
industry
but
also
nutraceutical
sectors.
Therefore,
this
review
aims
provide
overview
sources
potential
contributions
Journal of CO2 Utilization,
Год журнала:
2024,
Номер
80, С. 102669 - 102669
Опубликована: Янв. 21, 2024
The
impact
of
whiting
events
on
global
inorganic
carbon
cycle
and
climate
change
is
debatable
at
the
current
ocean
pH.
This
work
engineered
in
a
high
pH,
alkalinity
microalgal
culture
for
dioxide
capture
storage.
When
growing
marine
microalga
Nannochloropsis
oceanica
IMET1
photobioreactors,
more
than
doubled,
increasing
from
72.5
mg
L-1
to
maximum
level
159.6
L-1.
At
same
time,
pH
increased
7.9
10.1
with
concomitant
calcium
carbonate
production.
X-ray
diffraction
analysis
revealed
precipitated
was
primarily
monohydrocalcite.
lab
scaled
up
340-L
bioreactor,
which
ash-free
dry
weight
productivities
ranged
25.1
51.4
g
m-2
d-1
monohydrocalcite
productivity
133.4
m2
recorded.
In
this
system,
biomass
contained
about
23.9
%
lipids
eicosapentaenoic
acid
content
1.8
%.
Together,
these
results
suggest
microalgae-driven
production
process
efficiently
captures
stores
atmospheric
form
while
producing
valuable
bioproducts.
Study
bacterial
communities
associated
identified
four
dominant
species
Maricaulis
maris,
Marinisubtilis
pacificus,
Gracilimonas
sp.,
an
uncultured
bacterium
OD1
phylum
interesting
features
that
warrant
further
investigation.
Journal of Water Process Engineering,
Год журнала:
2024,
Номер
59, С. 104904 - 104904
Опубликована: Фев. 8, 2024
Aquaculture
streams
contain
nutrients
that
can
cause
eutrophication
problems
if
released
to
the
environment.
However,
microalgae
absorb
these
compounds
from
water.
In
this
study,
an
autotrophic
bloom
was
cultured
outdoors
in
a
High-Rate
Algal
Pond
(20
m2)
using
aquaculture
stream.
The
process
proved
be
technically
feasible,
removing
90
%
of
(nitrogen
and
phosphorus).
addition,
characterisation
produced
showed
suitability
replace
fish
meal
Solea
senegalensis
fattening
feeds
(up
21.4
inclusion).
techno-economic
analysis
at
large
scale
(10.19
ha)
resulted
water
treatment
cost
varying
1.37
1.66
€
m−3
depending
on
assumptions.
capital
costs
were
mainly
driven
by
production
stage
(76–84
equipment),
while
labour
represented
28–36
operational
expenditures.
case
biomass
sold
as
high-value
ingredient
for
feed,
total
could
covered
between
21
81
%.
