Microbiology Spectrum,
Год журнала:
2023,
Номер
11(1)
Опубликована: Янв. 11, 2023
Within
months
of
the
COVID-19
pandemic
being
declared
on
March
20,
2020,
novel,
more
infectious
variants
SARS-CoV-2
began
to
be
detected
in
geospatially
distinct
regions
world.
With
international
travel
a
lead
cause
spread
disease,
importance
rapidly
identifying
entering
country
is
critical.
In
this
study,
we
utilized
wastewater-based
epidemiology
(WBE)
monitor
presence
wastewater
generated
managed
quarantine
facilities
for
air
passengers
United
Kingdom.
Specifically,
developed
multiplex
reverse
transcription
quantitative
PCR
(RT-qPCR)
assays
identification
defining
mutations
associated
with
Beta
(K417N),
Gamma
(K417T),
Delta
(156/157DEL),
and
Kappa
(E154K)
which
were
globally
prevalent
at
time
sampling
(April
July
2021).
The
sporadically
Beta,
Gamma,
0.7%,
2.3%,
0.4%
all
samples,
respectively.
variant
was
identified
13.3%
peak
detection
rates
concentrations
observed
May
2021
(24%),
concurrent
its
emergence
RT-qPCR
results
correlated
well
those
from
sequencing,
suggesting
that
PCR-based
good
predictor
presence;
although,
inadequate
probe
binding
may
false
positive
or
negative
results.
Our
findings
suggest
WBE
coupled
used
as
rapid,
initial
assessment
identify
emerging
borders
mass
quarantining
facilities.
IMPORTANCE
global
COVID-19,
it
essential
harmful
able
escape
vaccines
rapidly.
To
date,
gold
standard
assess
circulating
communities
has
been
sequencing
S
gene
whole
genome
SARS-CoV-2;
however,
approach
time-consuming
expensive.
two
duplex
detect
quantify
Delta,
variants.
validated
using
RNA
extracts
derived
samples
taken
showed
correlation
demonstrated
Kingdom
2021.
here
enable
variant-specific
within
2
h
after
extract
outbreak
rapid
response.
Clinical Microbiology Reviews,
Год журнала:
2023,
Номер
37(1)
Опубликована: Дек. 14, 2023
SUMMARY
Wastewater-based
surveillance
(WBS)
has
undergone
dramatic
advancement
in
the
context
of
coronavirus
disease
2019
(COVID-19)
pandemic.
The
power
and
potential
this
platform
technology
were
rapidly
realized
when
it
became
evident
that
not
only
did
WBS-measured
SARS-CoV-2
RNA
correlate
strongly
with
COVID-19
clinical
within
monitored
populations
but
also,
fact,
functioned
as
a
leading
indicator.
Teams
from
across
globe
innovated
novel
approaches
by
which
wastewater
could
be
collected
diverse
sewersheds
ranging
treatment
plants
(enabling
community-level
surveillance)
to
more
granular
locations
including
individual
neighborhoods
high-risk
buildings
such
long-term
care
facilities
(LTCF).
Efficient
processes
enabled
extraction
concentration
highly
dilute
matrix.
Molecular
genomic
tools
identify,
quantify,
characterize
its
various
variants
adapted
programs
applied
these
mixed
environmental
systems.
Novel
data-sharing
allowed
information
mobilized
made
immediately
available
public
health
government
decision-makers
even
public,
enabling
evidence-informed
decision-making
based
on
local
dynamics.
WBS
since
been
recognized
tool
transformative
potential,
providing
near-real-time
cost-effective,
objective,
comprehensive,
inclusive
data
changing
prevalence
measured
analytes
space
time
populations.
However,
consequence
rapid
innovation
hundreds
teams
simultaneously,
tremendous
heterogeneity
currently
exists
literature.
This
manuscript
provides
state-of-the-art
review
established
details
current
work
underway
expanding
scope
other
infectious
targets.
The Science of The Total Environment,
Год журнала:
2022,
Номер
835, С. 155347 - 155347
Опубликована: Апрель 21, 2022
Much
of
what
is
known
and
theorized
concerning
passive
sampling
techniques
has
been
developed
considering
chemical
analytes.
Yet,
historically,
biological
analytes,
such
as
Salmonella
typhi,
have
collected
from
wastewater
via
with
Moore
swabs.
In
response
to
the
COVID-19
pandemic,
re-emerging
a
promising
technique
monitor
SARS-CoV-2
RNA
in
wastewater.
Method
comparisons
disease
surveillance
using
composite,
grab,
for
detection
found
variety
materials
routinely
produced
qualitative
results
superior
grab
samples
useful
sub-sewershed
COVID-19.
Among
individual
studies,
concentrations
derived
samplers
demonstrated
heterogeneous
correlation
paired
composite
ranging
weak
(R2
=
0.27,
0.31)
moderate
0.59)
strong
0.76).
sampler
materials,
electronegative
membranes
shown
great
promise
linear
uptake
observed
exposure
durations
24
48
h
several
cases
positivity
on
par
samples.
Continuing
development
methods
infectious
diseases
diverse
forms
fecal
waste
should
focus
optimizing
efficient
recovery
kit-free
extraction,
resource-efficient
testing
capable
rapidly
producing
or
quantitative
data.
With
refinements
could
prove
be
fundamental
tool
scaling
disease,
especially
among
1.8
billion
persons
living
low-resource
settings
served
by
non-traditional
collection
infrastructure.
PLOS Global Public Health,
Год журнала:
2023,
Номер
3(1), С. e0001346 - e0001346
Опубликована: Янв. 19, 2023
It
is
well
established
that
air
travel
plays
a
key
role
in
the
global
spread
of
many
enteric
and
respiratory
diseases,
including
COVID-19.
Even
with
restrictions
(e.g.
mask
wearing,
negative
COVID-19
test
prior
to
departure),
SARS-CoV-2
may
be
transmitted
by
asymptomatic
or
pre-symptomatic
individuals
carrying
virus.
Due
limitation
current
clinical
surveillance
approaches,
complementary
methods
need
developed
allow
estimation
frequency
entry
across
international
borders.
Wastewater-based
epidemiology
(WBE)
represents
one
such
approach,
allowing
unbiased
sampling
carriage
passenger
cohorts
entering
via
airports.
In
this
study,
we
monitored
sewage
samples
from
terminals
(n
=
150)
aircraft
32)
at
three
major
airports
UK
for
1–3
weeks
March
2022.
As
raw
were
more
turbid
than
typical
municipal
wastewater,
used
beef
extract
treatment
followed
polyethylene
glycol
(PEG)
precipitation
concentrate
viruses,
reverse
transcription
quantitative
PCR
(RT-qPCR)
detection
faecal
indicator
virus,
crAssphage.
All
taken
sewers
arrival
Heathrow
Bristol
airports,
85%
sites
Edinburgh
airport,
positive
SARS-CoV-2.
This
suggests
high
prevalence
among
passengers
and/or
airport
staff
members.
Samples
derived
also
showed
93%
positivity.
No
difference
viral
was
found
before
after
lifted.
Our
results
suggest
WBE
useful
tool
monitoring
transfer
rate
human
pathogens
other
disease-causing
agents
borders
should
form
part
wider
efforts
monitor
contain
future
disease
outbreaks.
Environmental Science & Technology,
Год журнала:
2023,
Номер
57(35), С. 12969 - 12980
Опубликована: Авг. 23, 2023
Wastewater-based
testing
(WBT)
for
SARS-CoV-2
has
rapidly
expanded
over
the
past
three
years
due
to
its
ability
provide
a
comprehensive
measurement
of
disease
prevalence
independent
clinical
testing.
The
development
and
simultaneous
application
WBT
measured
biomarkers
research
activities
pursuit
public
health
goals,
both
areas
with
well-established
ethical
frameworks.
Currently,
practitioners
do
not
employ
standardized
review
process,
introducing
potential
adverse
outcomes
professionals
community
members.
To
address
this
deficiency,
an
interdisciplinary
workshop
developed
framework
structured
WBT.
employed
consensus
approach
create
as
set
11
questions
derived
from
primarily
guidance.
This
study
retrospectively
applied
these
monitoring
programs
covering
emergent
phase
pandemic
(3/2020-2/2022
(
The Science of The Total Environment,
Год журнала:
2023,
Номер
872, С. 162116 - 162116
Опубликована: Фев. 10, 2023
During
the
last
two
years,
various
restrictions
have
been
set
up
to
limit
transmission
of
Coronavirus
Disease
(COVID-19).
While
these
rules
apply
at
a
large
scale
(e.g.,
country-wide
level)
human-to-human
virus
that
causes
COVID-19,
Severe
Acute
Respiratory
Syndrome
2
(SARS-CoV-2),
occurs
small
scale.
Different
preventive
policies
and
testing
protocols
were
implemented
in
buildings
where
COVID-19
poses
threat
elderly
residences)
or
constitutes
disruptive
force
schools).
In
this
study,
we
sampled
sewage
from
different
(a
school,
university
campus,
residence,
an
residence)
host
residents
levels
vulnerability.
Our
main
goal
was
assess
agreement
between
SARS-CoV-2
concentration
wastewater
applied
buildings.
All
using
passive
samplers
while
24-h
composite
samples
also
collected
residence.
Results
showed
performed
comparably
well
being
cost-effective
keep
track
prevalence.
comparison
sampling
(passive
vs.
active)
combined
with
strict
clinical
allowed
us
compare
sensitivities
methods.
Active
more
sensitive
than
sampling,
as
former
able
detect
prevalence
0.4
%,
compared
2.2
%
for
sampling.
The
number
COVID-19-positive
individuals
tracked
clinically
all
monitored
More
frequent
detection
observed
residential
non-residential
samplers.
buildings,
surveillance
can
be
used
complement
regimes,
remained
positive
even
when
no
reported.
Passive
is
useful
building
managers
adapt
their
mitigation
policies.
The Science of The Total Environment,
Год журнала:
2022,
Номер
847, С. 157548 - 157548
Опубликована: Июль 23, 2022
Based
on
recent
studies,
passive
sampling
is
a
promising
method
for
detecting
SARS-CoV-2
in
wastewater
surveillance
(WWS)
applications.
Passive
has
many
advantages
over
conventional
approaches.
However,
the
potential
benefits
of
are
also
coupled
with
apparent
limitations.
We
established
technique
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
using
electronegative
filters.
Though,
it
was
evident
that
adsorption
capacity
filters
constrained
their
use.
This
work
intends
to
demonstrate
an
optimized
granular
activated
carbon
(GAC).
Through
bench-scale
batch-adsorption
studies
and
sewershed
deployments,
we
characteristics
two
human
feacal
viruses
(PMMoV
CrAssphage)
onto
GAC.
A
pseudo-second-order
model
best-described
kinetics
either
deionized
(DI)
water
SARS-CoV-2,
CrAssphage,
PMMoV
wastewater.
In
both
laboratory
experiments
in-situ
maximum
amount
adsorbed
by
GAC
occurred
at
~60
h
wastewater,
CrAssphage
h.
contrast,
reached
DI
seeded
after
~35
The
equilibrium
assay
modeled
quantity
(q
Epidemiology and Infection,
Год журнала:
2023,
Номер
151
Опубликована: Янв. 1, 2023
Abstract
Wastewater
surveillance
and
quantitative
analysis
of
SARS-CoV-2
RNA
are
increasingly
used
to
monitor
the
spread
COVID-19
in
community.
We
studied
feasibility
applying
data
for
early
detection
local
outbreaks.
A
Monte
Carlo
simulation
model
was
constructed,
on
reported
variation
gene
copy
concentration
faeces
faecal
masses
shed.
It
showed
that,
even
with
a
constant
number
shedders,
concentrations
found
wastewater
samples
will
be
large,
that
it
challenging
translate
viral
into
incidence
estimates,
especially
when
shedders
is
low.
Potential
signals
hypothetical
outbreaks
were
analysed
their
performance
terms
sensitivity
specificity
signals.
The
results
suggest
sudden
increase
not
easily
identified
basis
data,
small
sampling
areas
low-incidence
situations.
However,
high
combining
from
multiple
consecutive
tests,
expected
improve
considerably.
developed
modelling
approach
can
our
understanding
SARS-CoV-2.