Graphene Oxide Nanoparticles and Organoids: A Prospective Advanced Model for Pancreatic Cancer Research

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(2), С. 1066 - 1066

Опубликована: Янв. 15, 2024

Pancreatic cancer, notorious for its grim 10% five-year survival rate, poses significant clinical challenges, largely due to late-stage diagnosis and limited therapeutic options. This review delves into the generation of organoids, including those derived from resected tissues, biopsies, pluripotent stem cells, adult as well advancements in 3D printing. It explores complexities tumor microenvironment, emphasizing culture media, integration non-neoplastic angiogenesis. Additionally, examines multifaceted properties graphene oxide (GO), such mechanical, thermal, electrical, chemical, optical attributes, their implications cancer diagnostics therapeutics. GO’s unique facilitate interaction with tumors, allowing targeted drug delivery enhanced imaging early detection treatment. The GO cultured organoid systems, particularly pancreatic research, is critically analyzed, highlighting current limitations future potential. innovative approach has promise transform personalized medicine, improve screening efficiency, aid biomarker discovery this aggressive disease. Through review, we offer a balanced perspective on prospects harnessing potential organoids GO.

Язык: Английский

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Exposure to MoS2 nanosheets or bulk activated Kruppel‐like factor 4 in 3D Caco‐2 spheroids in vitro and mouse intestines in vivo

Environmental Toxicology, Год журнала: 2023, Номер 38(8), С. 1925 - 1938

Опубликована: Апрель 26, 2023

MoS2 nanosheets (NSs) are novel 2D nanomaterials (NMs) being used in many important fields. Recently, we proposed the need to evaluate influences of NMs on Kruppel-like factors (KLFs) even if these materials relatively biocompatible. In this study, investigated NSs or bulk KLF4 signaling pathway 3D Caco-2 spheroids vitro and mouse intestines vivo. Through analysis our previous RNA-sequencing data, found that exposure activated expression spheroids. Consistently, also KLF4-related gene ontology (GO) terms down-regulated a panel KLF4-downstream genes. To verify findings, repeatedly exposed mice via intragastrical administration (1 mg/kg bodyweight, once day, for 4 days). It was shown oral decreased leading higher organ coefficients. As expected, both types increased Mo elements as well other trace elements, such Zn, Fe, Mn intestines. The induced morphological changes intestines, shortening intestinal villi crypt depth, which may result lipid staining. Consistent with material protein staining two downstream proteins, namely extracellular signal-regulated kinase (ERK) serine/threonine (AKT). We concluded were capable activate KLF4-signaling vivo vitro.

Язык: Английский

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Effects of multi‐walled carbon nanotubes and halloysite nanotubes on plasma lipid profiles and autophagic lipolysis pathways in mouse aortas and hearts

Environmental Toxicology, Год журнала: 2024, Номер 39(9), С. 4431 - 4446

Опубликована: Июнь 10, 2024

Abstract Multi‐walled carbon nanotubes (MWCNTs) and halloysite (HNTs) are widely used tubular‐structured nanomaterials (NMs), but their cardiovascular effects not clear. This study compared the of MWCNTs HNTs on lipid profiles in mouse plasma gene expression aortas hearts. Mice were intravenously injected with 50 μg NMs, once a day, for 5 days. Then, was collected lipidomics analysis, hearts RNA‐sequencing analysis. While or did induce obvious pathological changes hearts, altered. Further analysis revealed that more effectively upregulated sphingolipids sterol lipids, whereas glycerophospholipids fatty acyls. Consistently, data indicated altered signaling pathways related synthesis metabolism, as well those endoplasmic reticulum, lysosomes autophagy, significantly than We further verified proteins involved autophagic lipolysis, to suppress biomarker LC3, affect metabolism proteins. These results may provide novel understanding about influences systems. Importantly, previous studies considered biocompatible materials, from this suggested both capable lipolysis systems, although exact different.

Язык: Английский

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Nanomaterials in Organoids: From Interactions to Personalized Medicine

ACS Nano, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 28, 2024

Organoids are three-dimensional models of microscopic organisms created through the self-organization various types stem cells. They widely unitized in personalized medicine due to their capacity replicate structure and functionality native organs. Meanwhile, nanotechnology has been integrated into diagnostic therapeutic tools manage an array medical conditions, given its unique characteristics nanoscale. Nanomaterials have demonstrated potential developing innovative effective organoids. With a focus on studying interaction nanomaterials organoid technology medicine, this Review examines role regulating fate cells construct different It also explores create 3D microenvironments for Finally, perspectives challenges applying organoids development toward translation discussed.

Язык: Английский

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Graphene oxide exposure suppresses immune responses and increases the sensitivities of zebrafishes to lipopolysaccharides via the down-regulation of Toll-like receptors

Ecological Indicators, Год журнала: 2022, Номер 144, С. 109563 - 109563

Опубликована: Окт. 18, 2022

Besides the roles in regulating immune responses, recent studies have also suggested a pivotal role of Toll-like receptors (TLRs) lipid metabolism. Previously we reported that graphene oxide (GO) suppresses TLR signaling pathways. In this study, further investigated vivo toxicity GO zebrafish larvae via TLRs. Zebrafish embryos (5 h post fertilization; hpf) were exposed to 0.01, 0.1, 1 or 10 mg/L for up 5 days, and results revealed induced developmental toxicity, form delayed hatching time increased deformity, mortality heart rate; blood flow linear velocity, however, remained unchanged. Meanwhile, locomotor activities with exposure. As possible mechanism these effects, exposure significantly decreased TLR1, TLR4 TLR5 expression, leading suppressed expression genes, including interleukin-1β (IL-1β), IL-6, IL-8, tumor necrosis factor α (TNFα). Furthermore, perilipin 2 (PLIN2) was decreased, which consequently led droplet (LD) staining triglyceride (TG) content. Interestingly, GO-induced deformity by TLR2/TLR4 agonist lipopolysaccharides (LPS), but less effectively promoted TLR3 poly inosinic: cytidylic acid (IC). The from study induces suppression pathways, likely due inhibition dysfunction LD biogenesis.

Язык: Английский

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Mitigative Effect of Graphene Oxide Nanoparticles in Maintaining Gut–Liver Homeostasis against Alcohol Injury

Gastroenterology Insights, Год журнала: 2024, Номер 15(3), С. 574 - 587

Опубликована: Июль 2, 2024

Alcoholic liver disease (ALD) develops when the immunotolerant environment of is compromised due to excessive alcohol consumption. ALD progression involves variations in expressions multiple genes, resulting inflammation and development a leaky gut. It still unclear which molecular mechanism involved progression, that, there are currently no FDA-approved drugs available for its treatment. In this study, protective effects graphene oxide (GO) nanoparticles were investigated against ethanol-induced damage gut–liver axis vitro. GO was synthesized using modified Hummer’s method, characterization performed. Given general concerns regarding nanoparticle toxicity, assessments cell viability, lipid accumulation, DNA damage, death, generation reactive oxygen species (ROS) conducted various techniques. Furthermore, gene pro- anti-inflammatory cytokines determined RT-qPCR. The findings reveal that promoted viability even ethanol Additionally, accumulation significantly decreased cells treated with alongside compared treatment alone, similar trends observed other assays. A expression analysis indicated reduced proinflammatory while enhancing antioxidant genes. Moreover, led improvements gut integrity reduction colon damaged by ethanol. These suggest holds promise as drug carrier, exhibiting toxic effects. By shedding light on study contributes burgeoning field nanoparticle-mediated therapy ALD.

Язык: Английский

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Investigation of oral toxicity of WS₂ nanosheets to mouse intestine: Pathological injury, trace element balance, lipid profile changes, and autophagy

Journal of Applied Toxicology, Год журнала: 2024, Номер unknown

Опубликована: Сен. 29, 2024

Abstract The success of graphene oxides has gained extensive research interests in developing novel 2D nanomaterials (NMs). WS 2 nanosheets (NSs) are transition metal‐based NMs, but their toxicity is unclear. In this study, we investigated the oral NSs to mouse intestines. Male mice were administrated with vehicles, 1, 10, or 100 mg/kg via intragastric route, once a day, for 5 days. results indicate that did not induce pathological ultrastructural changes There minimal trace elements exposure W accumulation, and only Co levels dose‐dependently increased. Lipid droplets observed all groups mice, lipidomics data significantly decreased four lipid species, belonging phosphatidylcholine (PC). proteins regulating autophagic lipolysis, namely, LC3, lysosomal associated membrane protein (LAMP2) perilipin (PLIN2), increased, it was statistically different LC3. study suggest repeated induced influences on injury, element balance, autophagy, profiles intestines, indicating relatively high biocompatibility intestine route.

Язык: Английский

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Effects of Halloysite Nanotubes and Multi-walled Carbon Nanotubes on Kruppel-like Factor 15-Mediated Downstream Events in Mouse Hearts After Intravenous Injection

Cardiovascular Toxicology, Год журнала: 2024, Номер 24(4), С. 408 - 421

Опубликована: Фев. 27, 2024

Язык: Английский

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Organoid models for Chinese herbal medicine studies

Acta Materia Medica, Год журнала: 2023, Номер 2(1)

Опубликована: Янв. 1, 2023

Organoids are three-dimensional cell accusations generated from pluripotent stem cells or adult in vitro . With many advantages over and animal models, organoids have been increasingly used drug clinical medical research recent years. Chinese herbal medicine (CHM) is characterized by multi-target multi-pathway treatment methods; however, there no commonly accepted study method regarding efficacy underlying mechanisms. In this review we summarized the important applications of organoid models pharmacodynamic mechanism studies, safety evaluations, CHM personalized medicine, thus providing theoretical basis for its development innovation.

Язык: Английский

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Exposure of zebrafish (Danio rerio) to graphene oxide for 6 months suppressed NOD‐like receptor‐regulated anti‐virus signaling pathways

Environmental Toxicology, Год журнала: 2023, Номер 38(11), С. 2560 - 2573

Опубликована: Июль 14, 2023

Environmental exposure to graphene oxide (GO) is likely happen due the use and disposal of these materials. Although GO-induced ecological toxicity has been evaluated before by using aquatic models such as zebrafish, previous studies typically focused on short-term toxicity, whereas this study aimed investigate long-term toxicity. To end, we exposed zebrafish GO for 6 months, used RNA-sequencing reveal changes signaling pathways. While showed no significant effects locomotor activities, it induced histological in livers. data that altered gene expression profiles, resulting 82 up-regulated 275 down-regulated genes, respectively. Through analysis ontology terms Kyoto Encyclopedia Genes Genomes (KEGG) pathways, found suppressed pathways related with immune systems. We further verified genes involved anti-virus responses possibly through inhibition NOD-like receptor pathway. Furthermore, receptor-regulated lipid were also inhibited, which may consequently lead decreased staining fish muscles. concluded month-exposure zebrafish.

Язык: Английский

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