This
study
demonstrated
the
feasibility
of
enhanced
biological
phosphorus
removal
coupled
with
in-situ
fermentation
(EBPR-F)
to
improve
from
real
digested
swine
wastewater.
We
used
fermentable
substrates
(casein
hydrolysate
and
glucose)
as
external
carbon
sources
promote
enhance
removal.
Compared
conventional
EBPR
dominated
by
Candidatus
Accumulibacter,
EBPR-F
had
significantly
better
enriched
polyphosphate-accumulating
organisms
(PAOs).
Under
supplementation
100
mg/L
glucose,
total
(TP)
was
over
95%
in
EBPR-F,
an
average
TP
concentration
effluent
below
1.0
mg/L,
satisfying
discharge
standard
(8
mg
P/L)
China.
The
PAO
activity
relative
abundance
Accumulibacter
(44.7%
±
3.1%)
Tetrasphaera
(18.1%
6.6%)
were
much
higher
than
those
EBPR.
improvement
due
enrichment
through
fermentation,
can
efficiently
ferment
complex
organic
matter
provide
bioavailable
organics
for
Chemical Engineering Journal,
Год журнала:
2023,
Номер
468, С. 143700 - 143700
Опубликована: Май 24, 2023
The
implementation
of
a
side-stream
sludge
fermenter
(SSSF)
has
been
identified
as
possible
solution
to
improve
the
performance
an
anaerobic/anoxic/aerobic
(A2O)
configuration
when
treating
low
COD
wastewater.
This
study
systematically
evaluated
effects
incorporating
SSSF
into
A2O
(side-stream
enhanced
biological
phosphorus
removal,
S2EBPR)
for
P/N/COD
removal
under
limited
influent
(CODINF)
condition.
S2EBPR
(with
receiving
6%
recycled
activated
and
operating
with
hydraulic
retention
time
(HRT)
2.4
d)
were
compared
same
CODINF
(350
mg/L)
improved
amount
P
removed
(26.6%)
COD/P
only
26.3
32.6,
denitrification
(11%)
without
compromising
full
ammonium
removal.
However,
PLOAD
plant
increased
due
P-release
in
SSSF,
resulting
higher
effluent
concentration.
methane
energy
recovery
indexes
around
45%
lower
than
those
A2O.
Sequencing
analysis
revealed
high
abundance
PAO
accordance
its
represents
comprehensive
evaluation
provides
assessment
suitability.
ACS ES&T Water,
Год журнала:
2023,
Номер
3(8), С. 2384 - 2394
Опубликована: Май 26, 2023
Enhanced
biological
phosphorus
removal
(EBPR)
is
strongly
influenced
by
the
influent
ratio
of
readily
biodegradable
carbon
to
soluble
due
preferences
phosphorus-accumulating
organisms
(PAO).
The
sidestream
EBPR
(S2EBPR)
process
redirects
a
portion
return
activated
sludge
(RAS)
fermenter,
increasing
availability
carbon.
In
this
study,
we
assessed
performance
and
microbial
community
structure
full-scale
S2EBPR
demonstration
supplemented
with
external
dosing.
By
end
10
month
study
period,
achieved
median
effluent
orthophosphate
0.3
mg/L.
consisted
common
core
microbiome
in
RAS
basin,
nitrification
basin.
most
abundant
PAO
detected
were
Ca.
"Dechloromonas
phosphorivorans",
while
canonical
"Accumulibacter"
Tetrasphaera
observed
lower
relative
abundance.
addition
non-canonical
enrichment,
glycogen-accumulating
organism
"Competibacter"
proliferated
throughout
at
some
points
outnumbered
taxa
30
1
no
tangible
impacts.
This
provides
insights
into
successful
implementation
low-carbon
facility
improves
our
understanding
key
GAO
systems.
Water Science & Technology,
Год журнала:
2025,
Номер
91(5), С. 469 - 481
Опубликована: Март 1, 2025
ABSTRACT
Wastewater
treatment
facilities
use
enhanced
biological
phosphorus
removal
(EBPR)
to
meet
discharge
quality
limits.
However,
the
EBPR
process
can
experience
upsets
due
a
lack
of
influent
carbon
or
inadequate
anaerobic
zones.
By
using
sidestream
(S2EBPR)
process,
be
generated
internally
through
fermentation
processes
and
higher
mass
fraction
attained
in
smaller
volumes.
This
study
investigates
nutrient
microbial
community
trends
full-scale
S2EBPR
demonstration
at
Calumet
Water
Reclamation
Plant.
The
aims
improve
model
system
by
better
representing
activity
glycogen-accumulating
organisms
(GAO)
potential
competitors
phosphorus-accumulating
(PAO),
which
were
found
high
abundance
this
study.
Modifying
hydrolysis,
GAO
glycogen
storage
ORP
parameters
resulted
prediction
improvements
approximately
5%
for
nitrate
nitrite
10–60%
phosphorus.
also
uses
shotgun
metagenomic
sequencing
profile
denitrification
pathways
PAO
GAO.
It
shows
that
denitrifying
may
contribute
nitric
oxide
reduction
greater
degree
than
PAO.
improves
modeling
predictions
highlights
role
combined
nitrogen
S2EBPR.
ACS ES&T Water,
Год журнала:
2024,
Номер
4(2), С. 707 - 718
Опубликована: Янв. 19, 2024
The
discovery
of
the
complete
ammonia
oxidation
process
(comammox)
has
challenged
conventional
nitrification
theory,
showing
microbial
adaption
to
very
low
dissolved
oxygen
(DO)
concentrations.
This
study
aimed
at
investigating
effects
different
DO
concentrations
using
a
series
bioreactors
inoculated
with
biomass
from
three
operationally
diverse
water
resource
recovery
facilities.
Results
show
that
populations
adapted
environments
can
maintain
high
rates
across
range
concentrations,
indicating
their
ability
function
well
even
Additionally,
long
solids
retention
times
(>10
days)
encourage
persistence
comammox
Molecular
analyses
revealed
DO-facility
had
nitrifying
population
similar
ratios
clades
A
and
B,
while
facility
was
dominated
by
clade
A.
Modeling
results
suggest
including
bacteria
half-saturation
coefficient
for
(e.g.,
0.05
mg
L–1)
possible
intrapopulation
diversity
within
B.
highlights
changing
community
enable
activated
sludge
operate
effectively
leading
low-energy
biological
nitrogen
removal.
