Molecular Diagnosis & Therapy, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 2, 2024
Язык: Английский
Water Environment Research, Год журнала: 2025, Номер 97(5)
Опубликована: Май 1, 2025
Abstract Wastewater‐based surveillance (WBS) is valuable method for monitoring the dispersion of pathogens at a low cost. However, their impact on public health decision‐making limited because there lack long‐term analyses, especially in low‐ and middle‐income countries. This study aimed to assess effectiveness using WBS predict occurrence COVID‐19 waves estimate prevalence infection, emphasizing SARS‐CoV‐2 variants. During 17 months influent two wastewater treatment plants Queretaro City, Mexico, wave prediction time was influenced by variant dispersion. Waves dominated Delta Omicron variants circulation showed lead days values from 5 14 1 4 days, respectively. According Monte Carlo model, disease aligned with clinically reported cases onsets, but variant's transmissibility explained overestimation during peaks. work provides new insights into potential limitations as an epidemiological tool detecting predicting occurrence. Practitioner Points Long‐term allowed early case waves. The capability related presence infectivity. estimated higher all estimation has regarding variations data input.
Язык: Английский
Процитировано
0
Water Emerging Contaminants & Nanoplastics, Год журнала: 2025, Номер 4(2)
Опубликована: Май 26, 2025
In recent years, particularly following the COVID-19 pandemic, wastewater-based epidemiology (WBE) has emerged as an effective tool for early detection of disease outbreaks. This manuscript presents a novel perspective on WBE by highlighting sewage predictive instrument, capable providing near-real-time, community-level pathogen surveillance and anticipating mitigating future pandemics even before first clinical symptoms are detected. approach enables cost-effective, non-invasive, population-wide monitoring infectious diseases’ emergence, evolution, decline. By identifying pathogens in human waste (e.g., viruses bacteria), delivers real-time insights into infection trends, encompassing data from asymptomatic pre-symptomatic populations, enabling timely interventions public health authorities. Among key advantages its capacity to encompass large pinpoint transmission hotspots, facilitate resource allocation containment efforts. The efficacy predicting already been validated during potential critical component pandemic response preparedness. However, this also challenges such sample variability, environmental factors, infrastructure limitations. Through comprehensive review state-of-art available topic, including almost 300 published papers, present emphasizes expected impact integrating global frameworks discusses applications emerging diseases, aiming provide multidimensional overview integration with other tools.
Язык: Английский
Процитировано
0
PLoS ONE, Год журнала: 2025, Номер 20(5), С. e0322057 - e0322057
Опубликована: Май 28, 2025
Testing samples of wastewater for markers infectious disease became a widespread method surveillance during the COVID-19 pandemic. While these data generally correlate well with other indicators national prevalence, that cover localised regions tend to be highly variable over short time scales. Here we introduce procedure estimating real-time growth rate pathogen prevalence using series from sampling. The number copies target gene found in sample is modelled as time-dependent random whose distribution estimated maximum likelihood. output depends on hyperparameter controls sensitivity variability underlying data. We apply this reporting N1 SARS-CoV-2 collected at water treatment works across Scotland between February 2021 and 2023. all 121 sampling sites covering diverse range locations population sizes. find fitting natural determines its reliability detecting early stages an epidemic wave. Applying same hospital admissions data, changes are detected average 2 days earlier than admissions. In conclusion, paper provides robust generate reliable estimates responsive situational awareness inform public health.
Язык: Английский
Процитировано
0
Heliyon, Год журнала: 2024, Номер 10(5), С. e27452 - e27452
Опубликована: Март 1, 2024
The analysis of SARS-CoV-2 in wastewater has enabled us to better understand the spread and evolution virus worldwide. To deepen our understanding its epidemiological genomic characteristics, we analyzed 10,147 sequences from 5 continents 21 countries that were deposited GISAID database up until January 31, 2023. Our results revealed over 100 independent lineages circulating water samples March 2020 2023, including variants interest concern. We observed four clearly defined periods global distribution these time, with one variant being replaced by another. Interestingly, found water-borne different had a close phylogenetic relationship. Additionally, 40 Europe USA did not show any relationship human sequences. also identified significant number non-synonymous mutations, some which detected previously reported cryptic lineages. Among analyzed, France showed highest degree sequence diversity, while Austria genomes (6,296). study provides valuable information about diversity wastewater, can be employed support public health initiatives preparedness.
Язык: Английский
Процитировано
2
Journal of Applied Microbiology, Год журнала: 2024, Номер 135(7)
Опубликована: Июль 1, 2024
Abstract Aims This study aimed to assess the use of cross-assembled phage (crAssphage) as an endogenous control employing a multivariate normalization analysis and its application severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) data normalizer. Methods Results A total 188 twelve-hour composite raw sewage samples were obtained from eight wastewater treatment plants (WWTP) during 1-year monitoring period. Employing N1 N2 target regions, SARS-CoV-2 RNA was detected in 94% (177) 90% (170) samples, respectively, with global median 5 log10 genomic copies per liter (GC l−1). CrAssphage 100% ranging 8.29 10.43 GC l−1, 9.46 ± 0.40 presenting both spatial temporal variabilities. Conclusions Although crAssphage revealed correlation clinical cases occurring period, by flow capita day each WWTP increased this correlation, corroborating importance normalizing surveillance disease trend monitoring.
Язык: Английский
Процитировано
2
Journal of Virological Methods, Год журнала: 2024, Номер 332, С. 115063 - 115063
Опубликована: Ноя. 14, 2024
Wastewater-based epidemiology (WBE) is a crucial tool for health and environmental monitoring, providing real-time data on public indicators by analysis of sewage samples. Ensuring the integrity these samples from collection to paramount. This study investigates effects different cold-storage conditions wastewater samples, focusing both microbiological markers (such as extractable nucleic acids, SARS-CoV-2, crAssphage) physicochemical parameters (including ammonium, orthophosphate, pH, conductivity, turbidity). Composite combined raw influent five treatment works in South Wales, UK, were stored at 4°C, -20°C, -80°C, subjected up six freeze-thaw cycles over one year. The found significant storage temperature preservation certain WBE markers, with best yield most frequently seen -80°C. However, majority showed no difference between -80°C or demonstrating that it may not always be necessary archive ultra-low temperatures, thus reducing CO
Язык: Английский
Процитировано
2
Revista Panamericana de Salud Pública, Год журнала: 2023, Номер 47, С. 1 - 1
Опубликована: Июль 3, 2023
Poliovirus infection causes paralysis in up to 1 200 infected persons. The use of safe and effective inactivated poliovirus vaccines live attenuated oral (OPVs) means that only two pockets wildtype type remain, Afghanistan Pakistan. However, OPVs can revert virulence, causing outbreaks circulating vaccine-derived (cVDPV). During 2020–2022, cVDPV 2 (cVDPV2) was responsible for 97–99% poliomyelitis cases, mainly Africa. Between January August 2022, cVDPV2 detected sewage samples Israel, the United Kingdom States America, where a case acute flaccid caused by also occurred. Pan American Health Organization has warned Brazil, Dominican Republic, Haiti Peru are at very high risk reintroduction an additional eight countries Latin America risk, following dropping vaccination rates (average 80% coverage 2022). Sabin monovalent OPV been used control VDPV2 outbreaks, but its could lead outbreaks. To address this issue, more genetically stable, novel OPV2 (nOPV2) developed against 2020 granted World Emergency Use Listing. Rolling out vaccine under Listing mass settings contain requires unique local regulatory operational preparedness.
Язык: Английский
Процитировано
4
Journal of Basic Microbiology, Год журнала: 2024, Номер 64(8)
Опубликована: Май 7, 2024
Abstract Wastewater‐based epidemiology provides temporal and spatial information about the health status of a population. The objective this study was to analyze report epidemiological dynamics severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) in province Tucumán, Argentina during second third waves disease 2019 (COVID‐19) between April 2021 March 2022. aimed quantify SARS‐CoV‐2 RNA wastewater, correlating it with clinically reported COVID‐19 cases. Wastewater samples ( n = 72) were collected from 16 sampling points located three cities Tucumán (San Miguel de Yerba Buena y Banda del Río Salí). Detection viral nucleocapsid markers (N1 gene) carried out using one‐step reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR). Viral loads determined for each positive sample standard curve. A correlation p < 0.05) observed load (copies/mL) confirmed cases at specific San (SP4, SP7, SP8) both months, May June. Indeed, high concurred peaks This method allowed us follow behavior infection epidemic outbreaks. Thus, wastewater monitoring is valuable indicator that enables anticipation increases tracking progress pandemic. genome‐based surveillance should be implemented as routine practice prepare any future surge infections.
Язык: Английский
Процитировано
1
Health Promotion Journal of Australia, Год журнала: 2024, Номер 35(4), С. 1302 - 1315
Опубликована: Май 15, 2024
Abstract Issue Addressed The implementation of quick response (QR) code check‐in compliance behaviour during the COVID‐19 pandemic featured in infection control strategies several global jurisdictions, but was particular interest Australian context, where it became mandated on a nationwide scale. We aimed to identify salient beliefs people hold toward complying with QR using Theory Planned Behaviour belief‐based framework. Methods An elicitation study open‐ended questions (Queensland; N = 93, M age 4.77 years, SD 13.62 and Victoria; 76, 44.92 11.63) prospective correlational two‐wave online survey 290, 38.99, 46.6% female 38.27, 53.4% female) were conducted. Results Qualitative data coded through an iterative content analysis, while quantitative analysed linear multiple regression. Behavioural, normative associated intention both samples. Variation across states also observed. Conclusions Across samples, positive outcomes consistently exhibited stronger associations than reported negative outcomes. Distinct differences emerged between two samples terms regression effects. So What? indicate individual experience may affect which guide behaviour, supporting potential efficacy health promotion campaigns tapping into context specific experiences if is be implemented as measure future.
Язык: Английский
Процитировано
1
Journal of Water and Health, Год журнала: 2024, Номер 22(11), С. 2171 - 2183
Опубликована: Окт. 18, 2024
ABSTRACT The emergence of COVID-19 in 2020 significantly enhanced the application wastewater monitoring for detecting SARS-CoV-2 circulation within communities. From October 2021 to 2022, we collected 406 samples weekly from Córdoba Central Pipeline Network (BG-WWTP) and six specific sewer manholes sanitary zones (SZs). Following WHO guidelines, processed detected RNA variants using real-time PCR. Monitoring at SZ level allowed development a viral activity flow map, pinpointing key areas tracking its temporal spread variant evolution. Our findings demonstrate that wastewater-based surveillance acts as sensitive indicator activity, imminent increases cases before they become evident clinical data. This study highlights effectiveness targeted both municipal levels identifying hotspots assessing community-wide circulation. Importantly, data shows environmental studies provide valuable insights into virus presence, independent case records, offer robust tool adapting future public health challenges.
Язык: Английский
Процитировано
1