The Science of The Total Environment,
Год журнала:
2023,
Номер
907, С. 167811 - 167811
Опубликована: Окт. 16, 2023
In
recent
years,
wastewater-based
epidemiology
(WBE)
has
emerged
as
a
valuable
and
cost-effective
tool
for
monitoring
the
prevalence
of
COVID-19.
Large-scale
efforts
have
been
implemented
in
numerous
countries,
primarily
focusing
on
sampling
at
entrance
wastewater
treatment
plants
(WWTPs)
to
cover
large
population.
However,
finer
spatial
scale,
such
neighborhood
level
(NGBs),
pose
new
challenges,
including
absence
composite
infrastructure
increased
uncertainty
due
dynamics
small
catchments.
This
study
aims
investigate
feasibility
accuracy
WBE
when
deployed
(sampling
sewers)
compared
city
WWTP).
To
achieve
this,
we
specific
stations
intraurban
scale
within
three
NGBs
Barcelona,
Spain.
The
period
covers
5th
6th
waves
COVID-19
Spain,
spanning
from
March
2021
2022,
along
with
WWTP
downstream
NGBs.
results
showed
strong
correlation
between
clinical
cases
SARS-CoV-2
loads
both
NGB
levels.
Notably,
during
wave,
which
was
dominated
by
Delta
variant,
were
higher
than
wave
(Omicron
variant),
despite
lower
number
recorded
wave.
correlations
stronger
level.
early
warning
potential
varied
across
neighborhoods
waves,
some
showing
one-week
others
lacking
any
significant
signal.
Interestingly,
did
not
exhibit
major
differences
among
different
socioeconomic
statuses.
International Journal of Hygiene and Environmental Health,
Год журнала:
2024,
Номер
259, С. 114379 - 114379
Опубликована: Апрель 15, 2024
Wastewater
analysis
can
serve
as
a
source
of
public
health
information.
In
recent
years,
wastewater-based
epidemiology
(WBE)
has
emerged
and
proven
useful
for
the
detection
infectious
diseases.
However,
insights
from
wastewater
treatment
plant
do
not
allow
small-scale
differentiation
within
sewer
system
that
is
needed
to
analyze
target
population
under
study
in
more
detail.
Small-scale
WBE
offers
several
advantages,
but
there
been
no
systematic
overview
its
application.
The
aim
this
scoping
review
provide
comprehensive
current
state
knowledge
on
diseases,
including
methodological
considerations
A
database
search
was
conducted,
considering
only
peer-reviewed
articles.
Data
analyses
included
quantitative
summary
qualitative
narrative
synthesis.
Of
2130
articles,
we
278,
most
which
were
published
since
2020.
studies
analyzed
at
building
level
(n
=
203),
especially
healthcare
110)
educational
facilities
80),
neighborhood
scale
86).
main
analytical
parameters
viruses
178),
notably
SARS-CoV-2
161),
antibiotic
resistance
(ABR)
biomarkers
99),
often
by
polymerase
chain
reaction
(PCR),
with
DNA
sequencing
techniques
being
less
common.
terms
sampling
techniques,
active
dominated.
frequent
lack
detailed
information
specification
selection
criteria
characterization
sites
identified
concern.
conclusion,
based
large
number
studies,
overarching
strategic
aspects
WBE.
An
enabling
environment
requires
inter-
transdisciplinary
sharing
across
countries.
Promoting
adoption
will
benefit
common
international
conceptualization
approach,
standardized
internationally
accepted
terminology.
particular,
development
good
practices
different
warranted.
This
includes
establishment
guidelines
local
sub-sewersheds,
transparent
reporting
ensure
comparability
results.
Water,
Год журнала:
2024,
Номер
16(9), С. 1220 - 1220
Опубликована: Апрель 25, 2024
Background:
Wastewater-based
epidemiology
(WBE)
has
become
crucial
for
early
microbial
outbreak
detection
and
public
health
surveillance
globally,
underscored
by
the
COVID-19
pandemic.
However,
despite
advancements
in
sampling
analyses,
interpreting
results
estimating
infection
rates
pose
challenges.
Enhancements
sewer
system
engineering,
understanding
wastewater
environment,
addressing
impact
of
environment
on
accuracy
are
needed.
Objective:
This
scoping
review
aims
to
identify
engineering
knowledge
gaps
WBE
guide
future
study
designs.
Design:
Research
“wastewater-based
epidemiology”
involving
“engineering”,
published
between
2015
2023,
was
extracted
from
Scopus
database.
Results:
examines
elements
influencing
WBE’s
precision
reliability,
especially
identifying
measuring
SARS-CoV-2
RNA.
It
identifies
significant
effects
analytical
practices,
wastewater’s
composition
performance.
Conclusions:
calls
further
investigation
into
economical
evaluation
methods
these
factors
enhance
data
normalization
interpretation,
utilizing
existing
treatment
plant
used
control,
which
could
be
a
cost-effective
approach
over
more
expensive
population
biomarkers.
approach,
aside
SARS-CoV-2,
holds
potential
application
broader
number
types
diseases,
as
well
consumption
habits.
Environmental Science and Pollution Research,
Год журнала:
2023,
Номер
30(32), С. 79315 - 79334
Опубликована: Июнь 7, 2023
Abstract
Wastewater-based
epidemiology
has
been
widely
used
as
a
cost-effective
method
for
tracking
the
COVID-19
pandemic
at
community
level.
Here
we
describe
COVIDBENS,
wastewater
surveillance
program
running
from
June
2020
to
March
2022
in
treatment
plant
of
Bens
A
Coruña
(Spain).
The
main
goal
this
work
was
provide
an
effective
early
warning
tool
based
help
decision-making
both
social
and
public
health
levels.
RT-qPCR
procedures
Illumina
sequencing
were
weekly
monitor
viral
load
detect
SARS-CoV-2
mutations
wastewater,
respectively.
In
addition,
own
statistical
models
applied
estimate
real
number
infected
people
frequency
each
emerging
variant
circulating
community,
which
considerable
improved
strategy.
Our
analysis
detected
6
waves
with
concentrations
between
10
3
RNA
copies/L.
system
able
anticipate
outbreaks
during
8–36
days
advance
respect
clinical
reports
and,
emergence
new
variants
such
Alpha
(B.1.1.7),
Delta
(B.1.617.2),
Omicron
(B.1.1.529
BA.2)
42,
30,
27
days,
respectively,
before
did.
Data
generated
here
helped
local
authorities
managers
give
faster
more
efficient
response
situation,
also
allowed
important
industrial
companies
adapt
their
production
situation.
wastewater-based
developed
our
metropolitan
area
(Spain)
served
powerful
combining
monitoring
over
time.
The Science of The Total Environment,
Год журнала:
2023,
Номер
898, С. 165458 - 165458
Опубликована: Июль 14, 2023
Wastewater
surveillance
of
SARS-CoV-2
proved
useful,
including
for
identifying
the
local
appearance
newly
identified
virus
variants.
Previous
studies
focused
on
wastewater
treatment
plants
(WWTP)
with
sewersheds
several
hundred
thousand
people
or
at
single
building
level,
representing
only
a
small
number
people.
Both
approaches
may
prove
inadequate
small-scale
intra-urban
inferences
early
detection
emerging
novel
Our
study
aims
(i)
to
analyze
nucleotide
variants
(SNVs)
in
sub-sewersheds
and
WWTP
using
whole
genome
sequencing
order
(ii)
investigate
potential
known
concern
(VOC)
within
metropolitan
system.
We
selected
three
sub-sewershed
sampling
sites,
based
estimated
population-
built
environment-related
indicators,
inlet
receiving
Ruhr
region,
Germany.
Untreated
was
sampled
weekly
between
October
December
2021,
total
22
samples
collected.
RNA
analyzed
by
RT-qPCR
sequencing.
For
all
samples,
sequences
were
obtained,
while
13
positive
RT-qPCR.
multiple
specific
SNVs
WWTP.
Identified
reflected
dominance
VOC
Delta
time
sampling.
Interestingly,
we
could
identify
an
Omicron-specific
SNV
one
sub-sewershed.
A
concurrent
same
detected
Omicron
week
later.
observations
suggest
that
approach
particularly
useful
description
spatially
confined
existing
circulating
populations.
Future
applying
strategies
taking
into
account
features
system
will
be
temporal
spatial
variance
more
detail.
