Journal of Microbiological Methods,
Год журнала:
2024,
Номер
228, С. 107069 - 107069
Опубликована: Ноя. 17, 2024
Methods
that
are
used
to
characterise
microbiomes
and
antimicrobial
resistance
genes
(ARGs)
in
wastewater
not
standardised.
We
shotgun
metagenomic
sequencing
(SM-Seq),
RNA
(RNA-seq)
targeted
qPCR
compare
microbial
ARG
diversity
the
influent
a
municipal
treatment
plant
Australia.
ARGs
were
annotated
with
CARD-RGI
MEGARes
databases,
bacterial
was
characterised
by
16S
rRNA
gene
SM-Seq,
species
annotation
SILVA/GreenGenes
databases
or
Kraken2
NCBI
nucleotide
database
respectively.
CARD
identified
evenly
distributed
profiles
but
detected
richer
array
of
(richness
=
475
vs
320).
Qualitatively,
encoding
for
aminoglycoside,
macrolide-lincosamide-streptogramin
multidrug
most
abundant
all
examined
databases.
RNA-seq
only
32
%
there
concordance
qualitative
identification
macrolide-lincosamide,
phenicol,
sulfonamide
SM-Seq
RNA-seq.
confirmed
detection
some
ARGs,
including
OXA,
VEB
EREB,
influent.
For
bacteria,
equally
effective
population
profiling
at
phyla
class
level.
However,
significantly
higher
richness
15,000
3750).
These
results
demonstrate
sufficient
surveillance
wastewater.
more
precise
quantification
however,
presented
better
resolution.
The
functionality
confirmed,
general
agreement
on
putative
phenotypic
profile
observed
between
RNA-Seq
SM-Seq.
Veterinary Sciences,
Год журнала:
2025,
Номер
12(4), С. 297 - 297
Опубликована: Март 23, 2025
Antibiotic
resistance
represents
a
global
health
crisis
with
far-reaching
implications,
impacting
multiple
domains
concurrently,
including
human
health,
animal
and
the
natural
environment.
Wild
birds
were
identified
as
carriers
disseminators
of
antibiotic-resistant
bacteria
(ARB)
their
associated
antibiotic
genes
(ARGs).
A
majority
studies
in
this
area
have
concentrated
on
migratory
for
spread
over
long
distances.
However,
there
has
been
scant
research
resistome
non-migratory
Corvidae
species
that
heavily
overlap
activities,
which
limits
our
understanding
these
hinders
development
effective
management
strategies.
This
study
employed
metagenomics
approach
to
examine
characteristics
ARGs
mobile
genetic
elements
(MGEs)
five
common
inhabiting
Qinghai–Tibetan
Plateau.
The
classified
into
20
major
types
567
subtypes.
Notably,
multidrug
resistance,
macrolide–lincosamide–streptogramins,
tetracyclines,
beta-lactam,
bacitracin,
particularly
abundant,
subtypes
acrB,
bacA,
macB,
class
C
beta-lactamase,
tetA
being
especially
prevalent.
total
5
MGEs
(166
subtypes)
across
groups
crows,
transposase
genes,
indicated
presence
transposons,
most
abundant
type
MGEs.
Moreover,
some
opportunistic
pathogens
potential
hosts
Procrustes
analysis
co-occurrence
network
showed
composition
gut
microbiota
shaped
MGEs,
indicating
substantial
association
between
factors.
primary
mechanisms
crows
efflux
pumps,
alteration
targets,
enzymatic
inactivation.
High-risk
found
potentially
pose
significant
risks
public
also
analyzed
resulted
identification
81
Rank
I
47
II
ARGs.
Overall,
offers
comprehensive
characterization
wild
species,
enhancing
birds.
