Water Research,
Год журнала:
2025,
Номер
274, С. 123092 - 123092
Опубликована: Янв. 4, 2025
Chlorine,
the
most
widely
utilized
disinfectant
for
drinking
water
globally,
has
recently
been
implicated
in
facilitating
spread
of
antibiotic
resistance
genes
(ARGs),
raising
concerns
about
its
underestimated
environmental
and
ecological
risks.
However,
given
current
fragmented
research
focus
results,
a
comprehensive
understanding
potential
mechanisms
influencing
factors
behind
chlorination-promoted
ARGs
transmission
systems
is
crucial.
This
work
first
to
systematically
review
variations
abundance,
mechanisms,
factors,
mitigation
strategies
related
during
chlorination
process.
The
results
indicated
that
could
induce
genetic
mutations
promote
horizontal
gene
transfer
through
multiple
pathways,
including
increased
reactive
oxygen
species,
enhanced
membrane
permeability,
stimulation
SOS
response,
activation
efflux
pumps.
In
addition,
this
delves
into
significant
discoveries
regarding
affecting
ARG
water,
such
as
chlorine
concentration,
reaction
time,
disinfection
byproducts,
pipe
materials,
biofilms,
matrix.
A
series
effective
from
source
point-of-use
were
proposed
aimed
at
mitigating
risks
system.
Finally,
we
address
existing
challenges
outline
future
directions
overcome
these
bottlenecks.
Overall,
aims
advance
our
role
dissemination
inspire
innovative
ideas
optimizing
techniques,
minimizing
transmission,
enhancing
safety
water.
Abstract
Background
Antibiotics
and
microplastics
are
two
major
aquatic
pollutants
that
have
been
associated
to
antibiotic
resistance
selection
in
the
environment
considered
a
risk
human
health.
However,
little
is
known
about
interaction
of
these
at
environmental
concentrations
response
microbial
communities
plastisphere
sub-lethal
pollution.
Here,
we
describe
bacterial
dynamics
underlying
this
surface
water
bacteria
community,
resistome
mobilome
level
using
combination
methods
(next-generation
sequencing
qPCR),
targets
(16S
rRNA
gene,
pre-clinical
clinical
class
1
integron
cassettes
metagenomes),
technologies
(short
long
read
sequencing),
assembly
approaches
(non-assembled
reads,
genome
assembly,
bacteriophage
plasmid
assembly).
Results
Our
results
show
shift
community
antibiotics
microbiome
compared
subpopulations
respond
differently
microplastic
The
showed
an
increased
tolerance
selected
different
(ARB)
genes
(ARGs).
Several
metagenome
assembled
genomes
(MAGs)
derived
from
antibiotic-exposed
contained
ARGs,
virulence
factors,
involved
conjugation.
These
include
Comamonas,
Chryseobacterium,
opportunistic
pathogen
Stenotrophomonas
maltophilia
,
other
MAGs
belonging
genera
infections,
such
as
Achromobacter.
abundance
integron-associated
ciprofloxacin
gene
aac(6’)-Ib-cr
under
exposure
both
freshwater
plastisphere.
Regarding
mobilome,
although
no
significant
changes
ARG
load
integrons
plasmids
were
observed
polluted
samples,
identified
three
ARG-containing
viral
contigs
integrated
into
prophages.
Conclusions
This
study
illustrates
how
selective
nature
influences
pressure.
here
help
define
role
its
impact
on
maintenance
with
anthropogenic
pollutants.
research
highlights
need
evaluate
complex
scenarios
combined
stresses.
Foods,
Год журнала:
2024,
Номер
13(11), С. 1787 - 1787
Опубликована: Июнь 6, 2024
The
ubiquitous
presence
of
antimicrobial-resistant
organisms
and
antimicrobial
resistance
genes
(ARGs)
constitutes
a
major
threat
to
global
public
safety.
Tetracycline
(TET)
is
common
agent
that
inhibits
bacterial
growth
frequently
detected
in
aquatic
environments.
Although
TET
may
display
coselection
for
resistance,
limited
knowledge
available
on
whether
how
it
might
influence
plasmid-mediated
conjugation.
Subinhibitory
concentrations
(3.9–250
ng/mL)
promoted
horizontal
gene
transfer
(HGT)
via
the
mobilizable
plasmid
pVP52-1
from
donor
Vibrio
parahaemolyticus
NJIFDCVp52
recipient
Escherichia
coli
EC600
by
1.47-
3.19-fold.
transcription
levels
tetracycline
[tetA,
tetR(A)],
conjugation-related
(traA,
traD),
outer
membrane
protein
(ompA,
ompK,
ompV),
reactive
oxygen
species
(ROS)-related
(oxyR,
rpoS),
autoinducer-2
(AI-2)
synthesis
(luxS),
SOS-related
(lexA,
recA)
were
significantly
increased.
Furthermore,
overproduced
intracellular
ROS
generation
increased
cell
permeability
under
exposure
stimulated
conjugative
ARGs.
Overall,
this
study
provides
important
insights
into
contributions
spread
resistance.
Abstract
Arthropods,
such
as
houseflies,
play
a
significant
role
in
the
dissemination
of
antimicrobial
resistance
(AMR);
however,
their
impact
has
often
been
overlooked
comparison
to
other
AMR
vectors.
Understanding
contribution
arthropods
spread
is
critical
for
implementing
robust
policies
mitigate
across
One
Health
sectors,
affecting
animals
and
environmental
habitats
well
humans.
In
this
study,
we
investigated
situ
transfer
gfp-labelled
plasmid
(IncA/C
carrying
an
mcr-8
gene,
pA/C_MCR-8)
gut
microbiota
houseflies
(Musca
domestica)
by
applying
single-cell
sorting,
16S
rRNA
gene
amplicon
sequencing
whole-genome
sequencing.
Our
findings
demonstrate
that
pA/C_MCR-8–positive
Escherichia
coli
donor
strain
capable
colonizing
microbiome
persists
housefly
intestine
5
days;
no
was
detectable
above
detection
threshold
10−5
per
cell.
The
conjugative
pA/C_MCR-8
demonstrated
high
frequency
ranging
from
4.1
×
10−3
5.0
cell
vitro
exhibited
various
bacterial
phyla,
primarily
encompassing
Pseudomonadota
Bacillota.
Phylogenic
analysis
revealed
Providencia
stuartii,
human
opportunistic
pathogen,
notable
recipient
pA/C_MCR-8.
conjugation
assays
further
newly
formed
P.
stuartii
transconjugants
readily
clinically
relevant
pathogens
(e.g.
Klebsiella
pneumoniae).
indicate
potential
plasmids
support
adoption
approach
developing
infection
control
address
clinical
settings.
Poultry Science,
Год журнала:
2025,
Номер
104(6), С. 105082 - 105082
Опубликована: Март 23, 2025
Taihe
Black-Boned
Silky
Fowl
(TBSF)
is
a
unique
breed
in
China,
characterized
by
high
concentration
of
melanin
deposited
throughout
its
body.
Compared
to
broiler
chickens,
many
antibiotics
exhibit
significantly
longer
withdrawal
periods
TBSF.
Given
that
antibiotic
exposure
widely
recognized
as
the
primary
selective
pressure
driving
persistence
and
dissemination
resistance
genes
(ARGs)
across
diverse
environments,
it
crucial
investigate
occurrence
prevalence
ARGs
within
TBSF
farming
systems.
In
this
study,
34
Escherichia
coli
strains
isolated
from
22
farms
were
subjected
phenotypic
genotypic
analyses.
The
isolates
tested
for
susceptibility
28
antimicrobial
drugs
representing
nine
classes
determine
their
phenotypes.
Draft
genome
sequences
these
E.
obtained,
carried
mobile
genetic
elements,
particularly
plasmids,
analyzed
association
with
phenotype.
context
key
was
further
characterized.
Network
analysis
employed
correlations
between
ARGs,
phenotypes,
drug
residues.
results
demonstrated
rates
observed,
100
%
29.4
exhibiting
four
or
more
eight
classes,
respectively.
According
whole-genome
sequencing,
total
143
identified.
phenotypes
consistently
correlated
presence
corresponding
genomes.
β-lactams
resistant
mechanism
could
be
attributed
gene
blaTEM
and/or
blaOXA-10.
Similarly,
tetracyclines,
chloramphenicols,
aminoglycosides,
fluoroquinolones
fully
explained
tetR
tetA,
floR
cmlA,
ant(3'')-IIa,
aph(3'')-Ib,
aph(6)-Id,
aac(3)-IId,
aadA,
qnrS
mutant
gyrA/parC/mdtH.
majority
found
plasmid-associated.
This
study
verified
highlighted
prevalent
horizontal
transfer
farms.
Factors
such
hygiene
status,
biosecurity
measures,
other
environmental
conditions
might
play
significant
role
than
usage
facilitating
Appropriate
measures
should
taken
control
transmission
elements
associated
prevent
entry
into
human
clinical
environment
breeding
environment.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 2, 2025
The
extensive
use
of
antimicrobials
in
pig
farming
has
led
to
a
significant
emergence
antimicrobial
resistance
(AMR)
among
microorganisms.
Given
China's
prominent
position
as
the
leading
global
swine
producer,
AMR
become
focal
point
debates.
However,
limited
research
comprehensively
assessed
overall
status
Chinese
farms.
Samples
were
collected
from
31
provinces
China.
E.
coli
was
identified
and
isolated
using
McConkey's
selective
medium
confirmed
through
PCR
amplification.
MIC
value
determined
by
BD
Phoenix™
M50
Automated
Identification
Susceptibility
device.
J53
(sodium
azide-resistant)
used
recipient
conjugation
transfer
experiment.
phenotypic
genotypic
characteristics
isolates
analyzed
whole-genome
sequencing.
227
slurry
samples
52
farms
located
across
seven
geographical
regions
142
non-duplicate
strains
isolated,
their
phenotypes
28
systematically
evaluated.
ST10
ST641
widespread
sequence
types
with
numerous
genes
(ARGs)
virulence
factor
genes,
including
bla
NDM-1,
mcr-1.1,
OXA-10,
others.
Multiple
ARGs
co-located
on
single
plasmid,
an
analysis
genetic
context
revealed
insertion
sequences
adjacent
containing
various
mobile
elements.
Conjugation
experiments
provided
additional
evidence
for
horizontal
dissemination
these
ARGs.
release
into
environment
via
farm
comprises
emerging
contaminant
potential
hazard
public
health.
Consequently,
there
is
urgent
need
establish
universally
recognized
effluent
standards
monitoring
resistant
bacteria
