Chlorination-induced spread of antibiotic resistance genes in drinking water systems

Water Research, Journal Year: 2025, Volume and Issue: 274, P. 123092 - 123092

Published: Jan. 4, 2025

Chlorine, the most widely utilized disinfectant for drinking water globally, has recently been implicated in facilitating spread of antibiotic resistance genes (ARGs), raising concerns about its underestimated environmental and ecological risks. However, given current fragmented research focus results, a comprehensive understanding potential mechanisms influencing factors behind chlorination-promoted ARGs transmission systems is crucial. This work first to systematically review variations abundance, mechanisms, factors, mitigation strategies related during chlorination process. The results indicated that could induce genetic mutations promote horizontal gene transfer through multiple pathways, including increased reactive oxygen species, enhanced membrane permeability, stimulation SOS response, activation efflux pumps. In addition, this delves into significant discoveries regarding affecting ARG water, such as chlorine concentration, reaction time, disinfection byproducts, pipe materials, biofilms, matrix. A series effective from source point-of-use were proposed aimed at mitigating risks system. Finally, we address existing challenges outline future directions overcome these bottlenecks. Overall, aims advance our role dissemination inspire innovative ideas optimizing techniques, minimizing transmission, enhancing safety water.

Language: Английский

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Bacterial dynamics of the plastisphere microbiome exposed to sub-lethal antibiotic pollution

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: May 24, 2024

Abstract Background Antibiotics and microplastics are two major aquatic pollutants that have been associated to antibiotic resistance selection in the environment considered a risk human health. However, little is known about interaction of these at environmental concentrations response microbial communities plastisphere sub-lethal pollution. Here, we describe bacterial dynamics underlying this surface water bacteria community, resistome mobilome level using combination methods (next-generation sequencing qPCR), targets (16S rRNA gene, pre-clinical clinical class 1 integron cassettes metagenomes), technologies (short long read sequencing), assembly approaches (non-assembled reads, genome assembly, bacteriophage plasmid assembly). Results Our results show shift community antibiotics microbiome compared subpopulations respond differently microplastic The showed an increased tolerance selected different (ARB) genes (ARGs). Several metagenome assembled genomes (MAGs) derived from antibiotic-exposed contained ARGs, virulence factors, involved conjugation. These include Comamonas, Chryseobacterium, opportunistic pathogen Stenotrophomonas maltophilia , other MAGs belonging genera infections, such as Achromobacter. abundance integron-associated ciprofloxacin gene aac(6’)-Ib-cr under exposure both freshwater plastisphere. Regarding mobilome, although no significant changes ARG load integrons plasmids were observed polluted samples, identified three ARG-containing viral contigs integrated into prophages. Conclusions This study illustrates how selective nature influences pressure. here help define role its impact on maintenance with anthropogenic pollutants. research highlights need evaluate complex scenarios combined stresses.

Language: Английский

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Sulfonamide metabolites enhance resistance transmission via conjugative transfer pathways

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 491, P. 137932 - 137932

Published: March 13, 2025

Language: Английский

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Environmentally Relevant Concentrations of Tetracycline Promote Horizontal Transfer of Antimicrobial Resistance Genes via Plasmid-Mediated Conjugation

Foods, Journal Year: 2024, Volume and Issue: 13(11), P. 1787 - 1787

Published: June 6, 2024

The ubiquitous presence of antimicrobial-resistant organisms and antimicrobial resistance genes (ARGs) constitutes a major threat to global public safety. Tetracycline (TET) is common agent that inhibits bacterial growth frequently detected in aquatic environments. Although TET may display coselection for resistance, limited knowledge available on whether how it might influence plasmid-mediated conjugation. Subinhibitory concentrations (3.9–250 ng/mL) promoted horizontal gene transfer (HGT) via the mobilizable plasmid pVP52-1 from donor Vibrio parahaemolyticus NJIFDCVp52 recipient Escherichia coli EC600 by 1.47- 3.19-fold. transcription levels tetracycline [tetA, tetR(A)], conjugation-related (traA, traD), outer membrane protein (ompA, ompK, ompV), reactive oxygen species (ROS)-related (oxyR, rpoS), autoinducer-2 (AI-2) synthesis (luxS), SOS-related (lexA, recA) were significantly increased. Furthermore, overproduced intracellular ROS generation increased cell permeability under exposure stimulated conjugative ARGs. Overall, this study provides important insights into contributions spread resistance.

Language: Английский

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Housefly gut microbiomes as a reservoir and facilitator for the spread of antibiotic resistance

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Abstract Arthropods, such as houseflies, play a significant role in the dissemination of antimicrobial resistance (AMR); however, their impact has often been overlooked comparison to other AMR vectors. Understanding contribution arthropods spread is critical for implementing robust policies mitigate across One Health sectors, affecting animals and environmental habitats well humans. In this study, we investigated situ transfer gfp-labelled plasmid (IncA/C carrying an mcr-8 gene, pA/C_MCR-8) gut microbiota houseflies (Musca domestica) by applying single-cell sorting, 16S rRNA gene amplicon sequencing whole-genome sequencing. Our findings demonstrate that pA/C_MCR-8–positive Escherichia coli donor strain capable colonizing microbiome persists housefly intestine 5 days; no was detectable above detection threshold 10−5 per cell. The conjugative pA/C_MCR-8 demonstrated high frequency ranging from 4.1 × 10−3 5.0 cell vitro exhibited various bacterial phyla, primarily encompassing Pseudomonadota Bacillota. Phylogenic analysis revealed Providencia stuartii, human opportunistic pathogen, notable recipient pA/C_MCR-8. conjugation assays further newly formed P. stuartii transconjugants readily clinically relevant pathogens (e.g. Klebsiella pneumoniae). indicate potential plasmids support adoption approach developing infection control address clinical settings.

Language: Английский

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Strict relationship between phenotypic and plasmid-associated genotypic of multidrug-resistant Escherichia coli isolated from Taihe Black-Boned Silky Fowl farms

Poultry Science, Journal Year: 2025, Volume and Issue: 104(6), P. 105082 - 105082

Published: March 23, 2025

Taihe Black-Boned Silky Fowl (TBSF) is a unique breed in China, characterized by high concentration of melanin deposited throughout its body. Compared to broiler chickens, many antibiotics exhibit significantly longer withdrawal periods TBSF. Given that antibiotic exposure widely recognized as the primary selective pressure driving persistence and dissemination resistance genes (ARGs) across diverse environments, it crucial investigate occurrence prevalence ARGs within TBSF farming systems. In this study, 34 Escherichia coli strains isolated from 22 farms were subjected phenotypic genotypic analyses. The isolates tested for susceptibility 28 antimicrobial drugs representing nine classes determine their phenotypes. Draft genome sequences these E. obtained, carried mobile genetic elements, particularly plasmids, analyzed association with phenotype. context key was further characterized. Network analysis employed correlations between ARGs, phenotypes, drug residues. results demonstrated rates observed, 100 % 29.4 exhibiting four or more eight classes, respectively. According whole-genome sequencing, total 143 identified. phenotypes consistently correlated presence corresponding genomes. β-lactams resistant mechanism could be attributed gene blaTEM and/or blaOXA-10. Similarly, tetracyclines, chloramphenicols, aminoglycosides, fluoroquinolones fully explained tetR tetA, floR cmlA, ant(3'')-IIa, aph(3'')-Ib, aph(6)-Id, aac(3)-IId, aadA, qnrS mutant gyrA/parC/mdtH. majority found plasmid-associated. This study verified highlighted prevalent horizontal transfer farms. Factors such hygiene status, biosecurity measures, other environmental conditions might play significant role than usage facilitating Appropriate measures should taken control transmission elements associated prevent entry into human clinical environment breeding environment.

Language: Английский

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Microplastics Reduced the Natural Attenuation of Antibiotic Resistance Genes in Fertilized Soils

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 126144 - 126144

Published: March 1, 2025

Language: Английский

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Deciphering the profiles and hosts of antibiotic resistance genes and evaluating the risk assessment of general and non-general hospital wastewater by metagenomic sequencing

Environmental Pollution, Journal Year: 2025, Volume and Issue: 375, P. 126313 - 126313

Published: April 25, 2025

Language: Английский

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Whole genome sequencing based prediction of antimicrobial resistance evolution among the predominant bacterial pathogens of diabetic foot ulcer

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(5)

Published: May 1, 2025

Language: Английский

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Genomic epidemiology of antimicrobial resistance determinants in Chinese swine farm Escherichia coli isolates

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 2, 2025

The extensive use of antimicrobials in pig farming has led to a significant emergence antimicrobial resistance (AMR) among microorganisms. Given China's prominent position as the leading global swine producer, AMR become focal point debates. However, limited research comprehensively assessed overall status Chinese farms. Samples were collected from 31 provinces China. E. coli was identified and isolated using McConkey's selective medium confirmed through PCR amplification. MIC value determined by BD Phoenix™ M50 Automated Identification Susceptibility device. J53 (sodium azide-resistant) used recipient conjugation transfer experiment. phenotypic genotypic characteristics isolates analyzed whole-genome sequencing. 227 slurry samples 52 farms located across seven geographical regions 142 non-duplicate strains isolated, their phenotypes 28 systematically evaluated. ST10 ST641 widespread sequence types with numerous genes (ARGs) virulence factor genes, including bla NDM-1, mcr-1.1, OXA-10, others. Multiple ARGs co-located on single plasmid, an analysis genetic context revealed insertion sequences adjacent containing various mobile elements. Conjugation experiments provided additional evidence for horizontal dissemination these ARGs. release into environment via farm comprises emerging contaminant potential hazard public health. Consequently, there is urgent need establish universally recognized effluent standards monitoring resistant bacteria

Language: Английский

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