bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 5, 2023
Abstract
CRISPR-Cas9
technology
has
facilitated
development
of
strategies
that
can
potentially
provide
more
humane
and
effective
methods
to
control
invasive
vertebrate
species,
such
as
mice.
One
promising
strategy
is
X
chromosome
shredding
which
aims
bias
offspring
towards
males,
resulting
in
a
gradual
unsustainable
decline
females.
This
method
been
explored
insects
with
encouraging
results.
Here,
we
investigated
this
Mus
musculus
by
targeting
repeat
DNA
sequences
on
the
aim
inducing
sufficient
damage
specifically
eliminate
chromosome-bearing
sperm
during
gametogenesis.
We
tested
three
different
guide
RNAs
(gRNAs)
repeats
chromosome,
together
male
germline-specific
promoters
for
Cas9
expression
at
stages
spermatogenesis.
A
modest
mature
Y-bearing
was
detected
some
transgenic
although
did
not
translate
into
significant
male-biasing
offspring.
Instead,
cleavage
X-chromosome
meiosis
typically
resulted
spermatogenic
block,
manifest
small
testes
volume,
empty
tubules,
low
concentration,
sub/infertility.
Our
study
highlights
importance
controlling
timing
activity
mammalian
spermatogenesis
sensitivity
spermatocytes
disruption.
Australian Veterinary Journal,
Год журнала:
2023,
Номер
101(11), С. 453 - 459
Опубликована: Авг. 29, 2023
The
successful
rehabilitation
and
release
of
raptor
chicks
can
be
challenging,
especially
when
the
are
still
in
post-fledging
dependency
period.
Here,
we
report
on
a
recently
fledged
powerful
owl
chick
that
was
held
care
for
33
days
before
being
successfully
reunited
with
its
parents.
We
document
steps
undertaken
during
entire
process
from
collection
wild
to
post-release
monitoring
recommend
clinical
procedures
treatment
raptors
entering
veterinary
facilities.
Success
this
facilitated
by
early
potential
rodenticide
poisoning,
as
well
integration
citizen
scientists
family
unit
field
while
Given
emerging
evidence
widespread
poisoning
both
Australia
globally,
it
is
critical
suspect
all
may
have
been
exposed
anticoagulant
rodenticides
commence
vitamin
K
immediately.
Routine
increases
probability
recovery
post-trauma
reducing
time
much
possible.
Animals,
Год журнала:
2023,
Номер
13(24), С. 3809 - 3809
Опубликована: Дек. 10, 2023
Rodents
in
livestock
farming
constitute
a
threat
to
the
one
health
approach.
In
present
observational
case
study,
farmers
worked
together
with
pest
controller
within
pilot
project.
The
aim
of
study
was
assess
determinants
associated
rodent-prevention
potential.
started
March
2019
on
24
farms
municipality
North
Rhine-Westphalia/Germany.
At
beginning
project
survey
expected
be
related
prevention
potential
conducted.
To
determine
for
rodent
prevention,
an
expert
person,
who
not
involved
before,
assessed
on-site
conditions
after
1.5
years
duration
2020.
good
about
half
farms.
There
were
significant
differences
willingness
make
changes
at
project's
start
between
high
and
low
years.
is
general
need
action
provide
more
practical
information
control.
This
aggravated
by
insufficient
advisory
services
offered
farmers.
confirms
importance
implementing
preventive
measures
control
rodents
ensure
that
anticoagulant
rodenticides
are
handled
responsibly
reduce
impact
non-target
species.
Abstract
The
use
of
anticoagulant
rodenticides
(ARs)
to
manage
rodent
populations
can
result
in
unintentional
lethal
or
sublethal
poisoning
non-target
wildlife,
particularly
predatory
birds.
In
south-east
Queensland,
Australia,
infestations
fluctuate
due
favourable
environmental
conditions,
leading
increased
AR
and
incidences
secondary
poisoning.
Globally,
exposure
has
been
documented
However,
Australian
birds,
both
the
limits
impacts
are
poorly
understood.
This
study
examines
23
raptors
1
nightjar.
Postmortem
liver
samples
were
analysed
using
liquid
chromatography
mass
spectrometry
(LC–MS).
Traumatic
injuries
observed
15
remains
found
gizzards
9
individuals.
LC–MS
revealed
that
13
birds
had
ARs,
with
warfarin,
a
first-generation
(n
=
11)
being
most
common,
followed
by
second-generation
ARs
brodifacoum
3),
difethialone
1),
flocoumafen
1).
Only
six
thirteen
AR-positive
their
gastrointestinal
tracts,
highlighting
potential
bioaccumulation
associated
over
time.
contribution
death
these
unproven
underscores
need
for
ongoing
research
into
native
especially
areas
where
threatened
avifauna
inhabit
human-dominated
landscapes.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 5, 2023
Abstract
CRISPR-Cas9
technology
has
facilitated
development
of
strategies
that
can
potentially
provide
more
humane
and
effective
methods
to
control
invasive
vertebrate
species,
such
as
mice.
One
promising
strategy
is
X
chromosome
shredding
which
aims
bias
offspring
towards
males,
resulting
in
a
gradual
unsustainable
decline
females.
This
method
been
explored
insects
with
encouraging
results.
Here,
we
investigated
this
Mus
musculus
by
targeting
repeat
DNA
sequences
on
the
aim
inducing
sufficient
damage
specifically
eliminate
chromosome-bearing
sperm
during
gametogenesis.
We
tested
three
different
guide
RNAs
(gRNAs)
repeats
chromosome,
together
male
germline-specific
promoters
for
Cas9
expression
at
stages
spermatogenesis.
A
modest
mature
Y-bearing
was
detected
some
transgenic
although
did
not
translate
into
significant
male-biasing
offspring.
Instead,
cleavage
X-chromosome
meiosis
typically
resulted
spermatogenic
block,
manifest
small
testes
volume,
empty
tubules,
low
concentration,
sub/infertility.
Our
study
highlights
importance
controlling
timing
activity
mammalian
spermatogenesis
sensitivity
spermatocytes
disruption.
