Investigating the potential of X shredding for mouse genetic biocontrol DOI Creative Commons
Mark D. Bunting, G.I. Godahewa, Nicole O. McPherson

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 5, 2023

Abstract CRISPR-Cas9 technology has facilitated development of strategies that can potentially provide more humane and effective methods to control invasive vertebrate species, such as mice. One promising strategy is X chromosome shredding which aims bias offspring towards males, resulting in a gradual unsustainable decline females. This method been explored insects with encouraging results. Here, we investigated this Mus musculus by targeting repeat DNA sequences on the aim inducing sufficient damage specifically eliminate chromosome-bearing sperm during gametogenesis. We tested three different guide RNAs (gRNAs) repeats chromosome, together male germline-specific promoters for Cas9 expression at stages spermatogenesis. A modest mature Y-bearing was detected some transgenic although did not translate into significant male-biasing offspring. Instead, cleavage X-chromosome meiosis typically resulted spermatogenic block, manifest small testes volume, empty tubules, low concentration, sub/infertility. Our study highlights importance controlling timing activity mammalian spermatogenesis sensitivity spermatocytes disruption.

Язык: Английский

Farmers’ Attitudes in Connection with the Potential for Rodent Prevention in Livestock Farming in a Municipality in North Rhine-Westphalia, Germany DOI Creative Commons

Anna Schulze Walgern,

Odile Hecker,

Bernd Walther

и другие.

Animals, Год журнала: 2023, Номер 13(24), С. 3809 - 3809

Опубликована: Дек. 10, 2023

Rodents in livestock farming constitute a threat to the one health approach. In present observational case study, farmers worked together with pest controller within pilot project. The aim of study was assess determinants associated rodent-prevention potential. started March 2019 on 24 farms municipality North Rhine-Westphalia/Germany. At beginning project survey expected be related prevention potential conducted. To determine for rodent prevention, an expert person, who not involved before, assessed on-site conditions after 1.5 years duration 2020. good about half farms. There were significant differences willingness make changes at project's start between high and low years. is general need action provide more practical information control. This aggravated by insufficient advisory services offered farmers. confirms importance implementing preventive measures control rodents ensure that anticoagulant rodenticides are handled responsibly reduce impact non-target species.

Язык: Английский

Процитировано

1
Investigating the potential of X chromosome shredding for mouse genetic biocontrol DOI Creative Commons
Mark D. Bunting, G.I. Godahewa, Nicole O. McPherson

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июнь 12, 2024

Abstract CRISPR-Cas9 technology has facilitated development of strategies that can potentially provide more humane and effective methods to control invasive vertebrate species, such as mice. One promising strategy is X chromosome shredding which aims bias offspring towards males, resulting in a gradual unsustainable decline females. This method been explored insects with encouraging results. Here, we investigated this Mus musculus by targeting repeat DNA sequences on the aim inducing sufficient damage specifically eliminate chromosome-bearing sperm during gametogenesis. We tested three different guide RNAs (gRNAs) repeats chromosome, together male germline-specific promoters for Cas9 expression at stages spermatogenesis. A modest mature Y-bearing was detected some transgenic although did not translate into significant male-biasing offspring. Instead, cleavage meiosis typically resulted spermatogenic block, manifest small testes volume, empty tubules, low concentration, sub/infertility. Our study highlights importance controlling timing activity mammalian spermatogenesis sensitivity spermatocytes disruption.

Язык: Английский

Процитировано

0
Rodenticidas anticoagulantes: una amenaza ignorada para las aves rapaces de Argentina y otros países de Sudamérica DOI Creative Commons
Miguel D. Saggese, Valeria Ojeda, Gala Ortiz

и другие.

El Hornero, Год журнала: 2024, Номер 39(1), С. 7 - 33

Опубликована: Авг. 15, 2024

En Argentina, el riesgo que los rodenticidas anticoagulantes podrían presentar para las aves rapaces locales fue reconocido inicialmente en la década de 1980. Lamentablemente, 40 años después esta primera señal alarma, siguen siendo ampliamente utilizados país y toda Sudamérica, sin haberse estudiado problema medioambiental estos pueden suponer. Aquí presentamos una revisión práctica e integral sobre intoxicación por rapaces. Discutimos su impacto, tanto individuos como sus poblaciones, también aspectos relacionados al manejo animales intoxicados a necesidad contar con capacidad diagnóstica región. La información aquí recopilada permitirá contenidos relevantes, actualizados accesibles necesarios abordar estudio amenaza conservación Argentina otros países Sudamérica. Al mismo tiempo, esperamos promueva investigaciones tema permitan dar pasos evaluar mitigar uso puede tener otra fauna silvestre

Процитировано

0
Differential Exposure to Second-Generation Anticoagulant Rodenticides in Raptors from Continental and Insular Regions of the Iberian Peninsula DOI Creative Commons
Beatriz Martín-Cruz, Cristian Rial-Berriel,

Andrea Acosta Dacal

и другие.

Environmental Pollution, Год журнала: 2024, Номер unknown, С. 125034 - 125034

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

0
Evaluating sublethal anticoagulant rodenticide exposure in deceased predatory birds of South-East Queensland, Australia DOI Creative Commons
Zachary Low, Peter Murray,

Noman Naseem

и другие.

Deleted Journal, Год журнала: 2024, Номер 1(1)

Опубликована: Дек. 17, 2024

Abstract The use of anticoagulant rodenticides (ARs) to manage rodent populations can result in unintentional lethal or sublethal poisoning non-target wildlife, particularly predatory birds. In south-east Queensland, Australia, infestations fluctuate due favourable environmental conditions, leading increased AR and incidences secondary poisoning. Globally, exposure has been documented However, Australian birds, both the limits impacts are poorly understood. This study examines 23 raptors 1 nightjar. Postmortem liver samples were analysed using liquid chromatography mass spectrometry (LC–MS). Traumatic injuries observed 15 remains found gizzards 9 individuals. LC–MS revealed that 13 birds had ARs, with warfarin, a first-generation (n = 11) being most common, followed by second-generation ARs brodifacoum 3), difethialone 1), flocoumafen 1). Only six thirteen AR-positive their gastrointestinal tracts, highlighting potential bioaccumulation associated over time. contribution death these unproven underscores need for ongoing research into native especially areas where threatened avifauna inhabit human-dominated landscapes.

Язык: Английский

Процитировано

0
The Missing Toxic Link: Exposure of Non-Target Native Marsupials to Second-Generation Anticoagulant Rodenticides (Sgars) Suggest a Potential Route of Transfer into Apex Predators DOI

Kieran Scammell,

Raylene Cooke, Kaori Yokochi

и другие.

Опубликована: Янв. 1, 2023

Язык: Английский

Процитировано

0
Investigating the potential of X shredding for mouse genetic biocontrol DOI Creative Commons
Mark D. Bunting, G.I. Godahewa, Nicole O. McPherson

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 5, 2023

Abstract CRISPR-Cas9 technology has facilitated development of strategies that can potentially provide more humane and effective methods to control invasive vertebrate species, such as mice. One promising strategy is X chromosome shredding which aims bias offspring towards males, resulting in a gradual unsustainable decline females. This method been explored insects with encouraging results. Here, we investigated this Mus musculus by targeting repeat DNA sequences on the aim inducing sufficient damage specifically eliminate chromosome-bearing sperm during gametogenesis. We tested three different guide RNAs (gRNAs) repeats chromosome, together male germline-specific promoters for Cas9 expression at stages spermatogenesis. A modest mature Y-bearing was detected some transgenic although did not translate into significant male-biasing offspring. Instead, cleavage X-chromosome meiosis typically resulted spermatogenic block, manifest small testes volume, empty tubules, low concentration, sub/infertility. Our study highlights importance controlling timing activity mammalian spermatogenesis sensitivity spermatocytes disruption.

Язык: Английский

Процитировано

0