Investigating the potential of X shredding for mouse genetic biocontrol DOI Creative Commons
Mark D. Bunting, G.I. Godahewa, Nicole O. McPherson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 5, 2023

Abstract CRISPR-Cas9 technology has facilitated development of strategies that can potentially provide more humane and effective methods to control invasive vertebrate species, such as mice. One promising strategy is X chromosome shredding which aims bias offspring towards males, resulting in a gradual unsustainable decline females. This method been explored insects with encouraging results. Here, we investigated this Mus musculus by targeting repeat DNA sequences on the aim inducing sufficient damage specifically eliminate chromosome-bearing sperm during gametogenesis. We tested three different guide RNAs (gRNAs) repeats chromosome, together male germline-specific promoters for Cas9 expression at stages spermatogenesis. A modest mature Y-bearing was detected some transgenic although did not translate into significant male-biasing offspring. Instead, cleavage X-chromosome meiosis typically resulted spermatogenic block, manifest small testes volume, empty tubules, low concentration, sub/infertility. Our study highlights importance controlling timing activity mammalian spermatogenesis sensitivity spermatocytes disruption.

Language: Английский

Successful rehabilitation and release of a powerful owl chick with suspected rodenticide poisoning DOI Creative Commons
Raylene Cooke, Nicholas Carter, Jeremy Groves

et al.

Australian Veterinary Journal, Journal Year: 2023, Volume and Issue: 101(11), P. 453 - 459

Published: Aug. 29, 2023

The successful rehabilitation and release of raptor chicks can be challenging, especially when the are still in post-fledging dependency period. Here, we report on a recently fledged powerful owl chick that was held care for 33 days before being successfully reunited with its parents. We document steps undertaken during entire process from collection wild to post-release monitoring recommend clinical procedures treatment raptors entering veterinary facilities. Success this facilitated by early potential rodenticide poisoning, as well integration citizen scientists family unit field while Given emerging evidence widespread poisoning both Australia globally, it is critical suspect all may have been exposed anticoagulant rodenticides commence vitamin K immediately. Routine increases probability recovery post-trauma reducing time much possible.

Language: Английский

Citations

1

Farmers’ Attitudes in Connection with the Potential for Rodent Prevention in Livestock Farming in a Municipality in North Rhine-Westphalia, Germany DOI Creative Commons

Anna Schulze Walgern,

Odile Hecker,

Bernd Walther

et al.

Animals, Journal Year: 2023, Volume and Issue: 13(24), P. 3809 - 3809

Published: Dec. 10, 2023

Rodents in livestock farming constitute a threat to the one health approach. In present observational case study, farmers worked together with pest controller within pilot project. The aim of study was assess determinants associated rodent-prevention potential. started March 2019 on 24 farms municipality North Rhine-Westphalia/Germany. At beginning project survey expected be related prevention potential conducted. To determine for rodent prevention, an expert person, who not involved before, assessed on-site conditions after 1.5 years duration 2020. good about half farms. There were significant differences willingness make changes at project's start between high and low years. is general need action provide more practical information control. This aggravated by insufficient advisory services offered farmers. confirms importance implementing preventive measures control rodents ensure that anticoagulant rodenticides are handled responsibly reduce impact non-target species.

Language: Английский

Citations

1

Investigating the potential of X chromosome shredding for mouse genetic biocontrol DOI Creative Commons
Mark D. Bunting, G.I. Godahewa, Nicole O. McPherson

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: June 12, 2024

Abstract CRISPR-Cas9 technology has facilitated development of strategies that can potentially provide more humane and effective methods to control invasive vertebrate species, such as mice. One promising strategy is X chromosome shredding which aims bias offspring towards males, resulting in a gradual unsustainable decline females. This method been explored insects with encouraging results. Here, we investigated this Mus musculus by targeting repeat DNA sequences on the aim inducing sufficient damage specifically eliminate chromosome-bearing sperm during gametogenesis. We tested three different guide RNAs (gRNAs) repeats chromosome, together male germline-specific promoters for Cas9 expression at stages spermatogenesis. A modest mature Y-bearing was detected some transgenic although did not translate into significant male-biasing offspring. Instead, cleavage meiosis typically resulted spermatogenic block, manifest small testes volume, empty tubules, low concentration, sub/infertility. Our study highlights importance controlling timing activity mammalian spermatogenesis sensitivity spermatocytes disruption.

Language: Английский

Citations

0

Differential Exposure to Second-Generation Anticoagulant Rodenticides in Raptors from Continental and Insular Regions of the Iberian Peninsula DOI Creative Commons
Beatriz Martín-Cruz, Cristian Rial-Berriel,

Andrea Acosta Dacal

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: unknown, P. 125034 - 125034

Published: Sept. 1, 2024

Language: Английский

Citations

0

The Missing Toxic Link: Exposure of Non-Target Native Marsupials to Second-Generation Anticoagulant Rodenticides (Sgars) Suggest a Potential Route of Transfer into Apex Predators DOI

Kieran Scammell,

Raylene Cooke, Kaori Yokochi

et al.

Published: Jan. 1, 2023

Language: Английский

Citations

0

Investigating the potential of X shredding for mouse genetic biocontrol DOI Creative Commons
Mark D. Bunting, G.I. Godahewa, Nicole O. McPherson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 5, 2023

Abstract CRISPR-Cas9 technology has facilitated development of strategies that can potentially provide more humane and effective methods to control invasive vertebrate species, such as mice. One promising strategy is X chromosome shredding which aims bias offspring towards males, resulting in a gradual unsustainable decline females. This method been explored insects with encouraging results. Here, we investigated this Mus musculus by targeting repeat DNA sequences on the aim inducing sufficient damage specifically eliminate chromosome-bearing sperm during gametogenesis. We tested three different guide RNAs (gRNAs) repeats chromosome, together male germline-specific promoters for Cas9 expression at stages spermatogenesis. A modest mature Y-bearing was detected some transgenic although did not translate into significant male-biasing offspring. Instead, cleavage X-chromosome meiosis typically resulted spermatogenic block, manifest small testes volume, empty tubules, low concentration, sub/infertility. Our study highlights importance controlling timing activity mammalian spermatogenesis sensitivity spermatocytes disruption.

Language: Английский

Citations

0