bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 5, 2023
Abstract
CRISPR-Cas9
technology
has
facilitated
development
of
strategies
that
can
potentially
provide
more
humane
and
effective
methods
to
control
invasive
vertebrate
species,
such
as
mice.
One
promising
strategy
is
X
chromosome
shredding
which
aims
bias
offspring
towards
males,
resulting
in
a
gradual
unsustainable
decline
females.
This
method
been
explored
insects
with
encouraging
results.
Here,
we
investigated
this
Mus
musculus
by
targeting
repeat
DNA
sequences
on
the
aim
inducing
sufficient
damage
specifically
eliminate
chromosome-bearing
sperm
during
gametogenesis.
We
tested
three
different
guide
RNAs
(gRNAs)
repeats
chromosome,
together
male
germline-specific
promoters
for
Cas9
expression
at
stages
spermatogenesis.
A
modest
mature
Y-bearing
was
detected
some
transgenic
although
did
not
translate
into
significant
male-biasing
offspring.
Instead,
cleavage
X-chromosome
meiosis
typically
resulted
spermatogenic
block,
manifest
small
testes
volume,
empty
tubules,
low
concentration,
sub/infertility.
Our
study
highlights
importance
controlling
timing
activity
mammalian
spermatogenesis
sensitivity
spermatocytes
disruption.
Australian Veterinary Journal,
Journal Year:
2023,
Volume and Issue:
101(11), P. 453 - 459
Published: Aug. 29, 2023
The
successful
rehabilitation
and
release
of
raptor
chicks
can
be
challenging,
especially
when
the
are
still
in
post-fledging
dependency
period.
Here,
we
report
on
a
recently
fledged
powerful
owl
chick
that
was
held
care
for
33
days
before
being
successfully
reunited
with
its
parents.
We
document
steps
undertaken
during
entire
process
from
collection
wild
to
post-release
monitoring
recommend
clinical
procedures
treatment
raptors
entering
veterinary
facilities.
Success
this
facilitated
by
early
potential
rodenticide
poisoning,
as
well
integration
citizen
scientists
family
unit
field
while
Given
emerging
evidence
widespread
poisoning
both
Australia
globally,
it
is
critical
suspect
all
may
have
been
exposed
anticoagulant
rodenticides
commence
vitamin
K
immediately.
Routine
increases
probability
recovery
post-trauma
reducing
time
much
possible.
Animals,
Journal Year:
2023,
Volume and Issue:
13(24), P. 3809 - 3809
Published: Dec. 10, 2023
Rodents
in
livestock
farming
constitute
a
threat
to
the
one
health
approach.
In
present
observational
case
study,
farmers
worked
together
with
pest
controller
within
pilot
project.
The
aim
of
study
was
assess
determinants
associated
rodent-prevention
potential.
started
March
2019
on
24
farms
municipality
North
Rhine-Westphalia/Germany.
At
beginning
project
survey
expected
be
related
prevention
potential
conducted.
To
determine
for
rodent
prevention,
an
expert
person,
who
not
involved
before,
assessed
on-site
conditions
after
1.5
years
duration
2020.
good
about
half
farms.
There
were
significant
differences
willingness
make
changes
at
project's
start
between
high
and
low
years.
is
general
need
action
provide
more
practical
information
control.
This
aggravated
by
insufficient
advisory
services
offered
farmers.
confirms
importance
implementing
preventive
measures
control
rodents
ensure
that
anticoagulant
rodenticides
are
handled
responsibly
reduce
impact
non-target
species.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: June 12, 2024
Abstract
CRISPR-Cas9
technology
has
facilitated
development
of
strategies
that
can
potentially
provide
more
humane
and
effective
methods
to
control
invasive
vertebrate
species,
such
as
mice.
One
promising
strategy
is
X
chromosome
shredding
which
aims
bias
offspring
towards
males,
resulting
in
a
gradual
unsustainable
decline
females.
This
method
been
explored
insects
with
encouraging
results.
Here,
we
investigated
this
Mus
musculus
by
targeting
repeat
DNA
sequences
on
the
aim
inducing
sufficient
damage
specifically
eliminate
chromosome-bearing
sperm
during
gametogenesis.
We
tested
three
different
guide
RNAs
(gRNAs)
repeats
chromosome,
together
male
germline-specific
promoters
for
Cas9
expression
at
stages
spermatogenesis.
A
modest
mature
Y-bearing
was
detected
some
transgenic
although
did
not
translate
into
significant
male-biasing
offspring.
Instead,
cleavage
meiosis
typically
resulted
spermatogenic
block,
manifest
small
testes
volume,
empty
tubules,
low
concentration,
sub/infertility.
Our
study
highlights
importance
controlling
timing
activity
mammalian
spermatogenesis
sensitivity
spermatocytes
disruption.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 5, 2023
Abstract
CRISPR-Cas9
technology
has
facilitated
development
of
strategies
that
can
potentially
provide
more
humane
and
effective
methods
to
control
invasive
vertebrate
species,
such
as
mice.
One
promising
strategy
is
X
chromosome
shredding
which
aims
bias
offspring
towards
males,
resulting
in
a
gradual
unsustainable
decline
females.
This
method
been
explored
insects
with
encouraging
results.
Here,
we
investigated
this
Mus
musculus
by
targeting
repeat
DNA
sequences
on
the
aim
inducing
sufficient
damage
specifically
eliminate
chromosome-bearing
sperm
during
gametogenesis.
We
tested
three
different
guide
RNAs
(gRNAs)
repeats
chromosome,
together
male
germline-specific
promoters
for
Cas9
expression
at
stages
spermatogenesis.
A
modest
mature
Y-bearing
was
detected
some
transgenic
although
did
not
translate
into
significant
male-biasing
offspring.
Instead,
cleavage
X-chromosome
meiosis
typically
resulted
spermatogenic
block,
manifest
small
testes
volume,
empty
tubules,
low
concentration,
sub/infertility.
Our
study
highlights
importance
controlling
timing
activity
mammalian
spermatogenesis
sensitivity
spermatocytes
disruption.