Water,
Год журнала:
2024,
Номер
16(24), С. 3571 - 3571
Опубликована: Дек. 12, 2024
Antibiotic
resistance
is
a
silent
global
crisis
intensified
by
the
recent
pandemic
of
coronavirus
disease
2019
(COVID-19).
To
address
this
growing
threat,
wastewater-based
surveillance
(WBS)
emerging
as
promising
public
health
tool
for
monitoring
antibiotic
within
communities.
Our
meta-analysis
aims
to
reveal
landscape
antibiotic-resistance
genes
(ARGs)
in
wastewater
during
and
after
COVID-19
pandemic.
The
analysis
included
samples
collected
between
2020
2024
from
five
countries
across
three
continents:
Asia
(China),
Europe
(United
Kingdom
Russia),
North
America
States
Canada).
findings
showed
higher
observed
ARGs
Russia
China
despite
their
small
sample
size,
while
USA
more
diverse
ARGs.
Distinct
patterns
were
European
American
(p-value
<
0.001).
We
identified
2483
ARGs,
with
multidrug-resistant
(MDR)
dominating
most
regions
accounting
almost
45%
all
detected
Europe.
Country-specific
indicator
22
unique
Russia,
3
each
UK
Canada,
2
specific
China.
Continentally,
100
Asia,
38
Europe,
18
America.
These
highlight
regional
variations
ARG
profiles,
emphasizing
urgent
need
region-specific
strategies
combat
threat.
Additionally,
our
study
further
supports
value
WBS
valuable
resistance.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 24, 2025
The
COVID-19
pandemic
underscored
bacterial
resistance
as
a
critical
global
health
issue,
exacerbated
by
the
increased
use
of
antibiotics
during
crisis.
Notwithstanding
pandemic's
prevalence,
initiatives
to
address
medication
have
been
inadequate.
Although
an
overall
drop
in
worldwide
antibiotic
consumption,
total
usage
remains
substantial,
requiring
rigorous
regulatory
measures
and
preventive
activities
mitigate
emergence
resistance.
National
Action
Plans
(NAPs)
implemented
worldwide,
significant
disparities
persist,
particularly
low-
middle-income
countries
(LMICs).
Settings
such
farms,
hospitals,
wastewater
treatment
facilities,
agricultural
environments
include
presence
Antibiotic
Resistant
Bacteria
(ARB)
antibiotic-resistance
genes
(ARG),
promoting
propagation
Dietary
modifications
probiotic
supplementation
shown
potential
reshaping
gut
microbiota
reducing
gene
prevalence.
Combining
with
adjuvants
or
bacteriophages
may
enhance
efficacy
development.
Novel
therapeutic
approaches,
tailored
antibiotics,
monoclonal
antibodies,
vaccines,
nanoparticles,
offer
alternate
ways
addressing
In
spite
advancements
next-generation
sequencing
analytics,
gaps
persist
comprehending
role
regulating
Effectively
tackling
requires
robust
policy
interventions
targeting
root
causes
while
minimizing
public
risks.
This
review
provides
information
for
developing
strategies
protocols
prevent
colonization,
microbiome
resilience,
spread
International Journal of Environmental Research and Public Health,
Год журнала:
2024,
Номер
21(10), С. 1337 - 1337
Опубликована: Окт. 9, 2024
The
One
Health
concept
underscores
the
interconnectedness
of
human,
animal,
and
environmental
health,
necessitating
an
integrated,
transdisciplinary
approach
to
tackle
contemporary
health
challenges.
This
perspective
paper
explores
pivotal
role
genomics
bioinformatics
in
advancing
initiatives.
By
leveraging
genomic
technologies
tools,
researchers
can
decode
complex
biological
data,
enabling
comprehensive
insights
into
pathogen
evolution,
transmission
dynamics,
host-pathogen
interactions
across
species
environments
(or
ecosystems).
These
are
crucial
for
predicting
mitigating
zoonotic
disease
outbreaks,
understanding
antimicrobial
resistance
patterns,
developing
targeted
interventions
promotion
prevention.
Furthermore,
integrating
data
with
epidemiological
information
enhances
precision
public
responses.
Here
we
discuss
case
studies
demonstrating
successful
applications
contexts,
such
as
including
integration,
standardization,
ethical
considerations
research.
fostering
collaboration
among
geneticists,
bioinformaticians,
epidemiologists,
zoologists,
scientists,
harness
full
potential
safeguard
global
health.
necessity
continued
investment
interdisciplinary
education,
research
infrastructure,
policy
frameworks
effectively
employ
these
service
a
healthier
planet.
Microorganisms,
Год журнала:
2024,
Номер
12(1), С. 149 - 149
Опубликована: Янв. 12, 2024
Vancomycin-resistant
(VR)
Enterococcus
spp.
can
be
detected
in
high
concentrations
wastewaters
and
pose
a
risk
to
public
health.
During
one-year
study
(September
2022–August
2023),
24
h
composite
raw
wastewater
samples
(n
=
192)
of
municipal
treatment
plant
were
investigated
for
cultivable
enterococci.
After
growth
on
Slanetz–Bartley
agar
(SBA),
mean
concentration
29,736
±
9919
cfu/mL
was
calculated.
Using
MALDI-TOF
MS
characterize
randomly
picked
colonies
576),
the
most
common
species
found
faecium
(72.6%),
E.
hirae
(13.7%),
faecalis
(8.0%).
Parallel
incubation
SBA
VRESelect
resulted
rate
VR
enterococci
2.0
1.5%.
All
tested
strains
grown
172)
carried
vanA
(54.6%)
or
vanB
gene
(45.4%)
with
limited
sequence
differences.
In
susceptibility
experiments,
these
isolates
showed
high-level
resistance
vancomycin
(>256
µg/mL).
Concentration
determined
93.7%
112
(mean:
123.1
64.0
ng/L)
varied
between
below
100
ng/L
(the
detection
limit)
246.6
ng/L.
A
correlation
among
total
could
not
found.
The
combination
commercial
vancomycin-containing
applied
clinical
microbiology
multiplex
PCR
van
genes
is
an
easy-to-use
tool
quantify
water
samples.
International Journal of Environmental Research and Public Health,
Год журнала:
2025,
Номер
22(1), С. 97 - 97
Опубликована: Янв. 12, 2025
Antimicrobial
resistance
(AMR)
is
a
growing
global
health
threat.
This
study
investigated
antibiotic
in
E.
coli
isolates
from
municipal
wastewater
(86
isolates)
and
clinical
urinary
tract
infection
(UTI)
cases
(34
Grenadian
community,
using
data
January
2022
to
October
2023.
Antibiogram
data,
assessed
per
WHO
guidelines
for
Critically
Important
antimicrobials
(CIA),
showed
the
highest
levels
both
samples
ampicillin,
followed
by
amoxicillin/clavulanic
acid
nalidixic
acid,
all
classified
as
Important.
Similar
was
observed
sulfamethoxazole-trimethoprim
(highly
important)
groups,
with
nitrofurantoin
showing
important
category.
According
AWaRe
classification,
ampicillin
(ACCESS
group)
had
resistance,
while
lowest
across
samples.
The
WATCH
group
antibiotics,
cefuroxime
cefoxitin,
comparable
levels,
whereas
aztreonam
RESERVE
(tested
only
wastewater)
100%
sensitive.
Multiple
Antibiotic
Resistance
(MAR)
index
analysis
revealed
that
7%
of
38.2%
MAR
values
over
0.2,
indicating
prior
exposure
isolates.
These
parallel
patterns
highlight
monitoring
valuable
tool
AMR
surveillance,
supporting
stewardship
through
ongoing
environmental
assessment.
International Journal of Environmental Research and Public Health,
Год журнала:
2025,
Номер
22(3), С. 351 - 351
Опубликована: Фев. 27, 2025
Antimicrobial
resistance
(AMR)
poses
a
global
public
health
threat.
Wastewater
analysis
provides
valuable
insights
into
antimicrobial
genes
(ARGs),
identifying
sources
and
trends
evaluating
AMR
control
measures.
Between
February
2022
March
2023,
pre-treatment
urban
wastewater
samples
were
collected
weekly
from
treatment
plants
in
Pantano
D'Arci,
Siracusa,
Giarre
(Sicily,
Italy).
Monthly
composite
DNA
extracts
prepared
by
combining
subsamples
each
site,
yielding
42
samples-14
plant.
Real-time
PCR
targeted
specific
ARGs,
including
blaSHV,
erm(A),
erm(B),
blaOXA,
blaNDM,
blaVIM,
blaTEM,
blaCTX-M.
The
preliminary
findings
revealed
that
blaERM-B,
blaCTX-M
present
all
samples,
with
erm(B)
(median
value:
8.51;
range:
1.67-30.93),
blaSHV
(0.78;
0.00-6.36),
blaTEM
(0.72;
0.34-4.30)
showing
the
highest
relative
abundance.
These
results
underscore
importance
of
integrating
ARG
data
broader
research
to
understand
persistence
proliferation
mechanisms
ARGs
environments.
Future
studies
should
employ
metagenomic
analyses
profile
resistomes
urban,
hospital,
agricultural,
farm
wastewater.
Comparing
these
profiles
will
help
identify
contamination
pathways
inform
development
surveillance
programs.
Monitoring
shifts
abundance
could
signal
cross-sectoral
contamination,
enabling
more
effective
strategies.
