Insights into polyethylene biodegradative fingerprint of Pseudomonas citronellolis E5 and Rhodococcus erythropolis D4 by phenotypic and genome-based comparative analyses DOI Creative Commons
Jessica Zampolli, Elena Collina, Marina Lasagni

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 12

Опубликована: Дек. 12, 2024

Polyethylene (PE) is the most-produced polyolefin, and consequently, it most widely found plastic waste worldwide. PE biodegradation under study by applying different (micro)organisms in order to understand biodegradative mechanism majority of microbes. This aims identify novel bacterial species with compelling metabolic potential strategic genetic repertoires for biodegradation. Pseudomonas citronellolis E5 newly isolated from solid organic contaminated debris, Rhodococcus erythropolis D4 was selected its promising biodegradable determined repertoire. P. ability grow on as only carbon energy source. Meaningful extracellular secreted laccase activity also characterized during growth (E5 strains have a (2 ± 1)×10 –3 U mg −1 (3 , respectively). Despite highest level cell numbers recorded at 7 days both strains, patterns products obtained degraded 60 were dissimilar two bacteria sampling times. However, they mainly produced metabolites belonging carboxylic acids alkanes varying carbons aliphatic chains. Whole-genome sequence analyses compared R . determinant prediction (by gene annotation multiple alignment reference products) been performed, providing list 16 42 putatively related steps Altogether, these results support insights into genera perspectives base build up biotechnological platforms.

Язык: Английский

Sourcing chitin from exoskeleton of Tenebrio molitor fed with polystyrene or plastic kitchen wrap DOI Creative Commons
Larisa Ilijin, Maria Vesna Nikolić, Zorka Ž. Vasiljević

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 268, С. 131731 - 131731

Опубликована: Апрель 21, 2024

In this work we have characterized and compared chitin sourced from exoskeleton of Tenebrio molitor larvae fed with polystyrene or plastic kitchen wrap combined bran in the ratio 1: 1 only bran. Analysis frass by ATR-FTIR showed very similar spectra confirmed degradation feed components, while analysis verified absence any residue. Deproteinization followed demineralization produced 6.78-5.29 % chitin, showing that (polystyrene wrap) diet resulted heavier insect exoskeleton, but yielded slightly less lowest value obtained for diet. The deacetylation degree 98.17-98.61 was determined measured spectra. XRD presence α-chitin a crystallinity index 66.5-62 crystallite size 4-5 nm. Thermogravimetric curves all samples, two steps. These results show T. contributing to significant biodegradation major polluting materials can be feasible alternative source further promoting bio-circular economy.

Язык: Английский

Процитировано

5

Global perspectives on the biodegradation of LDPE in agricultural systems DOI Creative Commons
Jani E. Mendoza, Daniel Tineo, Beimer Chuquibala-Checan

и другие.

Frontiers in Microbiology, Год журнала: 2025, Номер 15

Опубликована: Янв. 7, 2025

The increasing use of plastics globally has generated serious environmental and human health problems, particularly in the agricultural sector where low-density polyethylene (LDPE) other are widely used. Due to its low recycling rate slow degradation process, LDPE is a major source pollution. This paper addresses problem plastic accumulation agriculture, focusing on biodegradation strategies. studies reviewed include recent data methodologies used state-of-the-art technologies others that have been for decades, monitor measure degree each treatment applied can have, including SEM, GCMS, HPLC, microscopy. countries investigating these identified, while some developing them years, only begun address this years. microorganisms such as bacteria, fungi, algae, insect larvae influence decomposition highlighted. A workflow proposed carry out type research. Despite advances, challenges remain, optimizing conditions accelerate process need further research delves into microbial interactions various contexts.

Язык: Английский

Процитировано

0

Biodegradation efficacy of Exiguobacterium sp. HSK30 towards vegetable packaging LDPE film DOI

Hemlata Sharma,

Deepesh Kumar Neelam

Biologia, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Язык: Английский

Процитировано

0

Multiobjective Optimization of the Economic Efficiency of Biodegradable Plastic Products: Carbon Emissions and Analysis of Geographical Advantages for Production Capacity DOI Open Access
Junpeng Zhang, Wei Zhong, Ning Chen

и другие.

Sustainability, Год журнала: 2025, Номер 17(7), С. 2874 - 2874

Опубликована: Март 24, 2025

The objective of this study was to address the limitations biodegradable plastics—low economic benefits and marketing difficulties. To end, analyzed production processes two plastics: polylactic acid (PLA) polybutylene adipate terephthalate (PBAT). Based on analysis, economic, technical, environmental improvement indicators were constructed, an optimization model with three objectives profit, carbon emission cost, process risk established. In study, we embedded improved NSGA-III algorithm obtain Pareto optimal solution set. We also proposed entropy-weighted efficiency index (EWEI) for analysis transport advantages based distribution plastics production, road density, regional prices. With a line capacity 10,000 tons 8% discount rate, 10-year return PBAT products 7,039,931.23 yuan higher than that PLA products. profit 488.92 per ton production. However, exhibited carbon-emission cost products, especially risk, by 0.11%. East China region has obvious geographical advantages, but Southwest is constrained in presence mountainous terrain. Therefore, it imperative optimize China’s overall industrial layout plastics, strengthen acquisition support sustainable promotion market, effectively minimize pollution caused traditional plastics.

Язык: Английский

Процитировано

0

Accelerating biodegradation efficiency of low-density polyethylene and its hazardous dissolved organic matter using unexplored polyolefin-respiring bacteria: New insights on degradation characterization, biomolecule influence and biotransformation pathways DOI

Maseed Uddin,

Swathi Krishnan Venkatesan,

Subhan Kumar Pal

и другие.

Journal of Hazardous Materials, Год журнала: 2025, Номер unknown, С. 138144 - 138144

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Deep Learning-Enhanced Hyperspectral Imaging for Rapid Screening of Co-Metabolic Microplastic-Degrading Bacteria in Environmental Samples DOI

Yuan Zheng,

Hao Zhou, Yingqi Peng

и другие.

Journal of Hazardous Materials, Год журнала: 2025, Номер unknown, С. 138370 - 138370

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Changes in the Chemical Composition of Polyethylene Terephthalate under UV Radiation in Various Environmental Conditions DOI Open Access
Sara Rostampour, Rachel Cook,

Song-Syun Jhang

и другие.

Polymers, Год журнала: 2024, Номер 16(16), С. 2249 - 2249

Опубликована: Авг. 8, 2024

Polyethylene terephthalate has been widely used in the packaging industry. Degraded PET micro(nano)plastics could pose public health concerns following release into various environments. This study focuses on degradation under ultraviolet radiation using NIST SPHERE facility at National Institute of Standards and Technology saturated humidity (i.e., ≥95% relative humidity) dry conditions ≤5% with varying temperatures (30 °C, 40 50 °C) for up 20 days. ATR-FTIR was to characterize chemical composition change degraded as a function UV exposure time. The results showed that cleavage ester bond peak 1713 cm−1 formation carboxylic acid 1685 were significantly influenced by radiation. Furthermore, considerably higher °C compared conditions. also more pronounced novelty this is provide insights environmental conditions, including radiation, humidity, temperature. can be develop strategies reduce impact plastic pollution.

Язык: Английский

Процитировано

3

A Positive Sample Enhancement Algorithm with Fuzzy Nearest Neighbor Hybridization for Imbalance Data DOI

Jiapeng Yang,

Lei Shi,

Tielin Lu

и другие.

International Journal of Fuzzy Systems, Год журнала: 2024, Номер unknown

Опубликована: Июнь 3, 2024

Язык: Английский

Процитировано

2

Changes in the Chemical Composition of Polyethylene Terephthalate Under UV Radiation in Various Environmental Conditions DOI Creative Commons
Sara Rostampour, Rachel Cook,

Song-Syun Jhang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Май 22, 2024

Abstract Polyethylene terephthalate has been widely used in the packaging industry. Degraded PET micro-nano plastics could pose public health concerns following release into various environments. This study focuses on degradation under ultraviolet radiation using NIST SPHERE facility at National Institute of Standards and Technology saturated humidity (i.e., ≥ 95 % relative humidity) dry conditions ≤ 5 with varying temperatures (30 °C, 40 50 °C) for up 20 days. ATR-FTIR was to characterize chemical composition change degraded as a function UV exposure time. The results showed that cleavage ester bond peak 1713 cm-1 formation carboxylic acid 1685 are significantly influenced by radiation. Furthermore, considerably higher °C compared conditions. also more pronounced novelty this is provide insights environmental conditions, including radiation, humidity, temperature. can be develop strategies reduce impact plastic pollution.

Язык: Английский

Процитировано

1

Multistep Enzymatic Low-density Polyethylene Degradation via Phenylalanine Monooxygenase and Isocitrate Lyase in Pseudomonas aeruginosa DOI Creative Commons
Hong Rae Kim,

Ye Eun Lee,

Dong-Eun Suh

и другие.

Опубликована: Июнь 4, 2024

Plastics have become indispensable in modern industries; however, their resistance to natural degradation poses environmental challenges. Biological technologies employing microorganisms offer promising solutions. Here, we analyzed the transcriptome and proteome of Pseudomonas aeruginosa, a plastic-degrading microorganism found gut superworms, identify genes enzymes upregulated during polyethylene degradation. Functional analyses these using Kyoto Encyclopedia Genes Genomes Gene Ontology databases revealed an increase lipid hydrophobic amino acid metabolism, suggesting involvement Based on analyses, identified phenylalanine monooxygenase, which is capable oxidizing plastics, isocitrate lyase, involved C-C bond cleavage. To investigate degradation, phhA aceA were transformed into Escherichia coli, produced purified. The purified then reacted with analyzed. results formation hydroxyl (-OH) C-O groups surface after treatment confirming its ability oxidize polyethylene. Isocitrate lyase alone did not affect production; when combined it contributed reduction molecular weight. This suggests two-stage process involving oxidation depolymerization that requires sequential action multiple enzymes. Thus, each stage demonstrated

Язык: Английский

Процитировано

1