A Lincomycin-Specific Antibody Was Developed Using Hapten Prediction, and an Immunoassay Was Established to Detect Lincomycin in Pork and Milk DOI Creative Commons

Yuhan Shang,

Dandan Zhang,

Yun Wen Shen

и другие.

Foods, Год журнала: 2024, Номер 13(19), С. 3118 - 3118

Опубликована: Сен. 29, 2024

Prolonged consumption of animal-derived foods containing high levels lincomycin (LIN) residues can adversely impact human health. Therefore, it is essential to develop specific antibodies and immunoassay methods for LIN. This study utilized computational chemistry predict the efficacy LIN haptens prior chemical synthesis, with subsequent confirmation obtained through an immunization experiment. A hybridoma cell line named LIN/1B11 was established, which The optimized indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method exhibited specificity detecting residues, IC50 value 0.57 ± 0.03 µg/kg. effectively detected in pork milk samples, achieving a limit detection (LOD) ranging from 0.81 1.20 µg/kg quantification (LOQ) 2.09 2.29 µg/kg, recovery rates between 81.9% 108.8%. offers valuable tool identifying food products. Furthermore, efficient hapten prediction presented herein improves antibody preparation efficiency provides simple researchers screening haptens.

Язык: Английский

Fate of antibiotic resistance genes and EPS defence mechanisms during simultaneous denitrification and methanogenesis, coupled with the biodegradation of multiple antibiotics under zinc stress DOI

Jierong Zheng,

Sufang Wang,

Qing Gong

и другие.

Water Research, Год журнала: 2024, Номер 261, С. 121996 - 121996

Опубликована: Июнь 24, 2024

Язык: Английский

Процитировано

10

Double-edged sword effects of sulfate reduction process in sulfur autotrophic denitrification system: Accelerating nitrogen removal and promoting antibiotic resistance genes spread DOI
Zhenyu Wang,

Changchun Yan,

Xuejiang Wang

и другие.

Bioresource Technology, Год журнала: 2024, Номер 409, С. 131239 - 131239

Опубликована: Авг. 8, 2024

Язык: Английский

Процитировано

10

Antibiotic resistance genes in anaerobic digestion: Unresolved challenges and potential solutions DOI
Chunxiao Wang, Xiaole Yin, Xiaoqing Xu

и другие.

Bioresource Technology, Год журнала: 2025, Номер unknown, С. 132075 - 132075

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1

Impact of in–situ bioelectric field on biogas production, membrane fouling and microbial community in an anaerobic membrane bioreactor under sulfadiazine stress DOI Creative Commons
Haojie Huang,

Yutong Sun,

Qing Du

и другие.

Chemical Engineering Journal, Год журнала: 2025, Номер 506, С. 160225 - 160225

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1

Polymerized ferric sulfate influences methane production from the anaerobic digestion of pig slurry: Performance and mechanism DOI

Liang-hui Hu,

Fang Chen,

Yue-gan Liang

и другие.

Fuel, Год журнала: 2025, Номер 389, С. 134296 - 134296

Опубликована: Фев. 12, 2025

Язык: Английский

Процитировано

1

Vertical variation of antibiotic resistance genes and their interaction with environmental nutrients in sediments of Taihu lake DOI
Jingjing Zhang, Juan Chen, Chao Wang

и другие.

Journal of Environmental Management, Год журнала: 2024, Номер 370, С. 122661 - 122661

Опубликована: Сен. 26, 2024

Язык: Английский

Процитировано

2

Deciphering Fe@C amendment on long-term anaerobic digestion of sulfate and propionate rich wastewater: Driving microbial community succession and propionate metabolism DOI
Jing Xie,

Rujing Lin,

Bolin Min

и другие.

Bioresource Technology, Год журнала: 2024, Номер 406, С. 130968 - 130968

Опубликована: Июнь 13, 2024

Язык: Английский

Процитировано

1

A Lincomycin-Specific Antibody Was Developed Using Hapten Prediction, and an Immunoassay Was Established to Detect Lincomycin in Pork and Milk DOI Creative Commons

Yuhan Shang,

Dandan Zhang,

Yun Wen Shen

и другие.

Foods, Год журнала: 2024, Номер 13(19), С. 3118 - 3118

Опубликована: Сен. 29, 2024

Prolonged consumption of animal-derived foods containing high levels lincomycin (LIN) residues can adversely impact human health. Therefore, it is essential to develop specific antibodies and immunoassay methods for LIN. This study utilized computational chemistry predict the efficacy LIN haptens prior chemical synthesis, with subsequent confirmation obtained through an immunization experiment. A hybridoma cell line named LIN/1B11 was established, which The optimized indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method exhibited specificity detecting residues, IC50 value 0.57 ± 0.03 µg/kg. effectively detected in pork milk samples, achieving a limit detection (LOD) ranging from 0.81 1.20 µg/kg quantification (LOQ) 2.09 2.29 µg/kg, recovery rates between 81.9% 108.8%. offers valuable tool identifying food products. Furthermore, efficient hapten prediction presented herein improves antibody preparation efficiency provides simple researchers screening haptens.

Язык: Английский

Процитировано

1