A Lincomycin-Specific Antibody Was Developed Using Hapten Prediction, and an Immunoassay Was Established to Detect Lincomycin in Pork and Milk DOI Creative Commons

Yuhan Shang,

Dandan Zhang,

Yun Wen Shen

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(19), P. 3118 - 3118

Published: Sept. 29, 2024

Prolonged consumption of animal-derived foods containing high levels lincomycin (LIN) residues can adversely impact human health. Therefore, it is essential to develop specific antibodies and immunoassay methods for LIN. This study utilized computational chemistry predict the efficacy LIN haptens prior chemical synthesis, with subsequent confirmation obtained through an immunization experiment. A hybridoma cell line named LIN/1B11 was established, which The optimized indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method exhibited specificity detecting residues, IC50 value 0.57 ± 0.03 µg/kg. effectively detected in pork milk samples, achieving a limit detection (LOD) ranging from 0.81 1.20 µg/kg quantification (LOQ) 2.09 2.29 µg/kg, recovery rates between 81.9% 108.8%. offers valuable tool identifying food products. Furthermore, efficient hapten prediction presented herein improves antibody preparation efficiency provides simple researchers screening haptens.

Language: Английский

Fate of antibiotic resistance genes and EPS defence mechanisms during simultaneous denitrification and methanogenesis, coupled with the biodegradation of multiple antibiotics under zinc stress DOI

Jierong Zheng,

Sufang Wang,

Qing Gong

et al.

Water Research, Journal Year: 2024, Volume and Issue: 261, P. 121996 - 121996

Published: June 24, 2024

Language: Английский

Citations

10
Double-edged sword effects of sulfate reduction process in sulfur autotrophic denitrification system: Accelerating nitrogen removal and promoting antibiotic resistance genes spread DOI
Zhenyu Wang,

Changchun Yan,

Xuejiang Wang

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 409, P. 131239 - 131239

Published: Aug. 8, 2024

Language: Английский

Citations

10
Antibiotic resistance genes in anaerobic digestion: Unresolved challenges and potential solutions DOI
Chunxiao Wang, Xiaole Yin, Xiaoqing Xu

et al.

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132075 - 132075

Published: Jan. 1, 2025

Language: Английский

Citations

1
Impact of in–situ bioelectric field on biogas production, membrane fouling and microbial community in an anaerobic membrane bioreactor under sulfadiazine stress DOI Creative Commons
Haojie Huang,

Yutong Sun,

Qing Du

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: 506, P. 160225 - 160225

Published: Jan. 1, 2025

Language: Английский

Citations

1
Polymerized ferric sulfate influences methane production from the anaerobic digestion of pig slurry: Performance and mechanism DOI

Liang-hui Hu,

Fang Chen,

Yue-gan Liang

et al.

Fuel, Journal Year: 2025, Volume and Issue: 389, P. 134296 - 134296

Published: Feb. 12, 2025

Language: Английский

Citations

1
Vertical variation of antibiotic resistance genes and their interaction with environmental nutrients in sediments of Taihu lake DOI
Jingjing Zhang, Juan Chen, Chao Wang

et al.

Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 370, P. 122661 - 122661

Published: Sept. 26, 2024

Language: Английский

Citations

2
Deciphering Fe@C amendment on long-term anaerobic digestion of sulfate and propionate rich wastewater: Driving microbial community succession and propionate metabolism DOI
Jing Xie,

Rujing Lin,

Bolin Min

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 406, P. 130968 - 130968

Published: June 13, 2024

Language: Английский

Citations

1
A Lincomycin-Specific Antibody Was Developed Using Hapten Prediction, and an Immunoassay Was Established to Detect Lincomycin in Pork and Milk DOI Creative Commons

Yuhan Shang,

Dandan Zhang,

Yun Wen Shen

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(19), P. 3118 - 3118

Published: Sept. 29, 2024

Prolonged consumption of animal-derived foods containing high levels lincomycin (LIN) residues can adversely impact human health. Therefore, it is essential to develop specific antibodies and immunoassay methods for LIN. This study utilized computational chemistry predict the efficacy LIN haptens prior chemical synthesis, with subsequent confirmation obtained through an immunization experiment. A hybridoma cell line named LIN/1B11 was established, which The optimized indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method exhibited specificity detecting residues, IC50 value 0.57 ± 0.03 µg/kg. effectively detected in pork milk samples, achieving a limit detection (LOD) ranging from 0.81 1.20 µg/kg quantification (LOQ) 2.09 2.29 µg/kg, recovery rates between 81.9% 108.8%. offers valuable tool identifying food products. Furthermore, efficient hapten prediction presented herein improves antibody preparation efficiency provides simple researchers screening haptens.

Language: Английский

Citations

1