Widespread anticoagulant resistance in house mice (Mus musculus musculus) linked to the Tyr139Phe mutation in the Czech Republic

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 11, 2025

Despite the widespread use of anticoagulant rodenticides in baits for controlling commensal rodent pests, their application is problematic due to secondary intoxication and increasing resistance. In contrast studies on Western European house mice (Mus musculus domesticus), few resistance have focused Eastern (M. musculus), which a western distribution boundary Czech Republic. This study newly analysed VKORC1 gene M. m. field populations from farms grain stores identified nonsynonymous mutation Tyr139Phe. was common throughout Republic present 80.2% 86 individuals sampled. Additionally, all exhibited genotype with three synonymous mutations specific subspecies musculus. The functional (mortality–survival) response Tyr139Phe validated laboratory choice feeding test using bromadiolone-based bait, where resistant homozygous survived, while susceptible died, mean survival 6.9 days.

Язык: Английский

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New polymorphisms of Vkork1 gene related to anticoagulant resistance of rats and mice in Italy

Pest Management Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

Abstract BACKGROUND Anticoagulant rodenticides (ARs) are a very effective tool to control rodent pest populations. Nevertheless, AR resistance has been documented worldwide. ARs block the cycle of vitamin K, leading death animal by internal bleeding: mutations in Vkorc1 gene can cause resistance. The spreading AR‐resistant rodents could lead an increase their populations, associated diffusion zoonotic pathogens, and amplified exposure non‐target animals ARs, thus it is important study its widely. This aimed report firstly presence synanthropic from Emilia‐Romagna region, Italy, evaluate role means molecular docking analysis. RESULTS A total 67 were analyzed: 24 Rattus norvegicus , 35 rattus eight Mus musculus . Single nucleotide polymorphisms (SNPs) with resistance, homozygosis or heterozygosis, detected 6/8 mice, on codons 128 139, 13/24 R. 61 10/35 codon 59. Furthermore, several newly described missense all tested species: analysis suggests some these (e.g., I123S F87L) brodifacoum, both rats mice. CONCLUSION discovery SNPs 43.28% sounds as alarm bell that requires introduction integrated approach, where not used routinely, but under specific monitoring evaluation. © 2024 Author(s). Pest Management Science published John Wiley & Sons Ltd behalf Society Chemical Industry.

Язык: Английский

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Mn-MOF based electrochemical sensor for highly detection of poisonous rat bait (Bromadiolone)

Inorganic Chemistry Communications, Год журнала: 2024, Номер 171, С. 113554 - 113554

Опубликована: Ноя. 15, 2024

Язык: Английский

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Museomics and morphological analyses of historical and contemporary peninsular Italian wolf (Canis lupus italicus) samples

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 4, 2025

Abstract After centuries of decline and protracted bottlenecks, the peninsular Italian wolf population has naturally recovered. However, an exhaustive comprehension effects such a conservation success is still limited by reduced availability historical data. Therefore, in this study, we morphologically genetically analyzed contemporary samples, also exploiting optimization innovative bone DNA extraction method, to describe morphological variability subspecies its genetic diversity during last 30 years. We obtained high amplification genotyping rates for tissue, blood petrous samples. Multivariate, clustering analyses confirmed that Apennine well-distinguishable from both European wolves dogs, with no natural immigration other populations, while remained low across three decades, without significant changes between specimens. This study highlights scientific value well-maintained museum collections, demonstrates bones represent reliable sources, emphasizes need long-term monitor dynamics peculiar populations ensure appropriate management actions.

Язык: Английский

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Can the Wolf (Canis lupus) Thrive in Highly Anthropised Lowlands? First Habitat Suitability Analysis of the Po Plain, Italy

Animals, Год журнала: 2025, Номер 15(4), С. 546 - 546

Опубликована: Фев. 13, 2025

Coexisting with large carnivores in human-dominated European landscapes is a highly relevant and current challenge. Over the last two centuries, wolf (Canis lupus) population Europe has experienced significant decline, primarily due to direct human persecution. However, recent conservation policies, combined species' remarkable ecological flexibility, have enabled rapid recovery. This process, which now also extends densely populated areas, exemplified by ongoing expansion across Po Plain, one of Europe's most heavily landscapes. Our study aims provide first assessment habitat suitability for presence Plain. Using ten-year dataset occurrences (2015-2024), we evaluated influence several environmental human-related factors applying models Maximum Entropy algorithm (MaxEnt). The goal was quantify potentially suitable habitats within plain identify that could either facilitate or constrain presence. results showed approximately half Plain Among anthropogenic variables, "urban areas" only factor significantly negatively affected suitability, while other variables had negligible impacts. underscores behavioural adaptability. By investigating distribution anthropised regions, this shed light on ability thrive human-altered landscapes, contributing efforts informing future strategies coexistence lowland ecosystems.

Язык: Английский

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One Health approach and tiered strategy to assess anticoagulant rodenticides exposure in red foxes (Vulpes vulpes) from Central Italy

Environmental Pollution, Год журнала: 2025, Номер unknown, С. 126403 - 126403

Опубликована: Май 1, 2025

Anticoagulant rodenticides (ARs) are widely used in urban and agricultural areas to control rodent populations, leading potential exposure of non-target species like red foxes (Vulpes vulpes). However, data on AR Italy remain limited. This study analyzed 92 fox liver samples for residues ten ARs, categorizing concentrations into five groups assess toxicological risks. The were also compared with the CORINE Land Cover dataset correlated infectious parasitic diseases, including respiratory intestinal infections. Residues at least one found 46 (50%), 25% containing AR, 20% two, 5% three. Second-generation anticoagulant (SGARs) more prevalent than first-generation anticoagulants (FGARs), detected 47.9% samples. Positive primarily from but settings. observed suggest biological risks, a significant correlation between ARs confirms Italian highlights need integrated risk mitigation strategies protect humans, animals, environmental health.

Язык: Английский

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Detection of Vkorc1 single nucleotide polymorphisms indicates the presence of anticoagulant rodenticide resistance in Australia's introduced rats^†

Pest Management Science, Год журнала: 2025, Номер unknown

Опубликована: Май 30, 2025

Abstract BACKGROUND Anticoagulant rodenticides (ARs) are used globally to manage pest rodent populations. However, resistance ARs in target populations challenges control efforts and can increase risks nontarget species. Resistance is frequently associated with nonsynonymous single nucleotide polymorphisms (nsSNPs) the Vkorc1 gene, this study carried out first survey of introduced rats on Australian mainland. RESULTS We identified three species rat using cytochrome b gene across Brisbane, Melbourne, Perth Sydney: Rattus rattus (Linnaeus 1758) (Lineage I); norvegicus (Berkenhout 1769); tanezumi (Temminck 1844) II). Three nsSNPs were detected gene: Tyr25Phe , Trp59Arg Phe55Ile . The mutation which ARs, was 58 108 R. (53.7%) one 31 (3.2%). It has been suggested that (detected two ) susceptibility haemorrhage, whereas (identified only not reported previously. No CONCLUSION This update status mainland since 1970s employ genetic screening. widespread occurrence urbanized areas Australia suggests potential common practical conferred by needs further investigation as does role hybridization transfer from nuclear genome. © 2025 Author(s). Pest Management Science published John Wiley & Sons Ltd behalf Society Chemical Industry.

Язык: Английский

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Exposure to anticoagulant rodenticides in steppe polecat (Mustela eversmanii) and European polecat (Mustela putorius) in central Europe

The Science of The Total Environment, Год журнала: 2024, Номер 948, С. 174282 - 174282

Опубликована: Июль 2, 2024

Poisoning caused by coumarin-type anticoagulant rodenticides (ARs) stands as the predominant method for controlling rodents globally. ARs, through secondary poisoning, pose a significant threat to predators due their lethal and sublethal effects. We examined concentration of accumulated ARs in liver samples mostly road-killed steppe polecats (Mustela eversmanii) European (M. putorius) collected throughout Hungary between 2005 2021. The polecat were found mainly from Eastern Hungary, while Western Hungary. measured six residues HPLC-FLD. Our analysis revealed presence one first-generation four second-generation 53% (36) 39% (26) samples. In 17 we detected at least two AR compounds. Although did not find variance accumulation species, displayed greater prevalence maximum whereas exhibited more diverse these Brodifacoum bromadiolone most prevalent ARs; highest concentrations 0.57 mg/kg 0.33 mg/kg, respectively. was positively correlated with human population density negatively extent natural habitats both species. To best our knowledge, this is first study demonstrate rodenticide exposure globally, Central region. appears comparatively lower than many other countries, issue poisoning remains serious problem intrude into food webs. Reduced prudent usage pesticides would provide several benefits wildlife, included humans. However, advocate prioritization ecosystem services complete prohibition toxicants.

Язык: Английский

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First evidence of the suitability of hair for assessing wildlife exposure to anticoagulant rodenticides (ARs)

Environmental Research, Год журнала: 2024, Номер 264, С. 120302 - 120302

Опубликована: Ноя. 5, 2024

Язык: Английский

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Spatial bias in dietary studies can limit our understanding of the feeding ecology of large carnivores

Опубликована: Июнь 26, 2024

Many large carnivores have broad geographical ranges, encompassing ecosystems with a different prey base. Our understanding of their diet could therefore be biased by the spatial concentration dietary studies into few areas. We propose protocol to divide range carnivores, areas that are homogeneous respect available food sources, using grey wolf (Canis lupus) in Italy, as case study. mapped potential maximum distribution wolves, on 10 km grid (n = 2,497), and then performed cluster analysis classify cells according their: i) abundance domestic wild ungulates, ii) suitability for coypu (Myocastor coypus) iii) landscape anthropization. Finally, we checked percentage each were covered 2007-2013, 2014-2018 2019-2023. The wolves Italy can divided 5 areas, characterized sources but also coverage from studies. Alps some sectors Apennines, low anthropization abundant oversampled. More anthropized Central Southern rich sheep well lowlands, waste coypu, undersampled. no study was carried out intensive farming districts Northern Italy. indicates future about should focus landscapes. There, consumption pets trigger persecution pathogen transmission, predation increase exposure toxic compounds. broadly, our improve feeding ecology it used to: assess put perspective meta-analytic findings, identify knowledge gaps arising bias prioritize new undersampled design sampling schemes large-scale research.

Язык: Английский

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