Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis
Diagnostics,
Год журнала:
2024,
Номер
14(9), С. 931 - 931
Опубликована: Апрель 29, 2024
Infectious
diseases
account
for
about
3
million
deaths
per
year.
The
advent
of
molecular
techniques
has
led
to
an
enormous
improvement
in
their
diagnosis,
both
terms
sensitivity
and
specificity
the
speed
with
which
a
clinically
useful
result
can
be
obtained.
Digital
PCR,
or
3rd
generation
is
based
on
series
technical
modifications
that
more
sensitive
techniques,
resistant
action
inhibitors
capable
direct
quantification
without
need
standard
curves.
This
review
presents
main
applications
have
been
developed
diagnosis
viral,
bacterial,
parasitic
infections
potential
prospects
clinical
use
this
technology.
Язык: Английский
Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis
Опубликована: Март 20, 2024
A
Infectious
diseases
account
for
about
3
million
deaths
per
year.
The
advent
of
molecular
techniques
has
led
to
an
enormous
improvement
in
their
diagnosis,
both
terms
sensitivity
and
specificity
the
speed
with
which
a
clinically
useful
result
can
be
obtained.
Digital
PCR,
or
3rd
generation
is
based
on
series
technical
modifications
that
more
sensitive
techniques,
resistant
action
inhibitors
capable
direct
quantification,
without
need
standard
curves.This
review
presents
main
applications
have
been
developed
diagnosis
viral,
bacterial
parasitic
infections,
potential
prospects
clinical
use
this
technology.
Язык: Английский
Sensitivity-Enhanced Hydrogel Digital RT-LAMP with In Situ Enrichment and Interfacial Reaction for Norovirus Quantification in Food and Water
Journal of Hazardous Materials,
Год журнала:
2025,
Номер
488, С. 137325 - 137325
Опубликована: Янв. 22, 2025
Язык: Английский
A review of antibiotic resistance genes in major river basins in China: Distribution, drivers, and risk
Environmental Pollution,
Год журнала:
2025,
Номер
unknown, С. 125920 - 125920
Опубликована: Фев. 1, 2025
Язык: Английский
Environmental occurrence of antibiotic resistance, control measures and challenges in finding therapeutic management
Emerging contaminants,
Год журнала:
2024,
Номер
unknown, С. 100440 - 100440
Опубликована: Ноя. 1, 2024
Язык: Английский
Simultaneous biomass concentration and subsequent quantitation of multiple infectious disease agents and antimicrobial resistance genes from community wastewater
Environment International,
Год журнала:
2024,
Номер
191, С. 108973 - 108973
Опубликована: Авг. 22, 2024
Wastewater-based
surveillance
(WBS)
of
infectious
disease
agents
is
increasingly
seen
as
a
reliable
source
population
health
data.
To
date,
wastewater-based
efforts
have
largely
focused
on
individual
pathogens.
However,
given
that
wastewater
contains
broad
range
pathogens
circulating
in
the
population,
more
comprehensive
approach
could
enhance
its
usability.
We
simultaneous
detection
SARS-CoV-2,
sapovirus,
Campylobacter
jejuni,
coli,
Salmonella
spp.,
pathogenic
Escherichia
Cryptosporidium
Giardia
spp.
and
antimicrobial
resistance
genes
(ARGs)
clinical
relevance.
achieve
this
goal,
biomass
concentration
nucleic
acid
extraction
methods
were
optimized,
samples
analyzed
by
using
set
(RT)-qPCR
(HT)-qPCR
methods.
determined
prevalence
spatial
temporal
trends
targeted
collected
novel
information
ARGs
Finnish
wastewater.
In
addition,
use
different
concentrates,
namely
ultrafiltered
concentrate
supernatant
centrifuged
pellet,
effect
freezing
thawing
prior
to
sample
processing
investigated
with
indicator
microbe
crAssphage.
Freeze-thawing
decreased
gene
copy
count
crAssphage
comparison
analyzing
fresh
(p
<
0.001).
Campylobacters
most
abundant
two
four
studied
summer
months
(30
%
rate)
wastewaters
from
regions
intensive
animal
farming.
Salmonella,
however,
was
detected
40
without
any
clear
seasonal
trends,
highest
numbers
recorded
largest
treatment
plants.
Beta-lactamase
commonly
been
bacteria
isolated
humans
Finland,
bla
Язык: Английский