Impact of Sample Storage Time and Temperature on the Stability of Respiratory Viruses and Enteric Viruses in Wastewater DOI Creative Commons
Yuanyuan Qiu,

Richardson Mah,

Logan A. Brand

и другие.

Microorganisms, Год журнала: 2024, Номер 12(12), С. 2459 - 2459

Опубликована: Ноя. 29, 2024

Wastewater-based surveillance (WBS) has been widely used to track SARS-CoV-2 as well many other viruses in communities during the COVID pandemic and post-pandemic. However, it is still not clear how temperature storage time would influence stability of wastewater. In this study, we assessed SARS-CoV-2, pepper mild mottle virus (PMMoV), influenza A (IAV) B (IBV), respiratory syncytial (RSV), enteric raw wastewater stored at room temperature, 4 °C, −20 °C for 3 6 days. PMMoV, IAV, were found be stable up days after storing or °C. RSV more susceptible freeze–thaw cycles compared PMMoV viruses, which relatively Low detection IBV made difficult evaluate impact. Based on our findings, conclude that short-term transportation samples within ambient acceptable majority these viruses. Freezing even short periods recommended WBS The data obtained from study can provide guidance quality assurance purposes operational aspects surveillance.

Язык: Английский

Impact of Sample Storage Time and Temperature on the Stability of Respiratory Viruses and Enteric Viruses in Wastewater DOI Creative Commons
Yuanyuan Qiu,

Richardson Mah,

Logan A. Brand

и другие.

Microorganisms, Год журнала: 2024, Номер 12(12), С. 2459 - 2459

Опубликована: Ноя. 29, 2024

Wastewater-based surveillance (WBS) has been widely used to track SARS-CoV-2 as well many other viruses in communities during the COVID pandemic and post-pandemic. However, it is still not clear how temperature storage time would influence stability of wastewater. In this study, we assessed SARS-CoV-2, pepper mild mottle virus (PMMoV), influenza A (IAV) B (IBV), respiratory syncytial (RSV), enteric raw wastewater stored at room temperature, 4 °C, −20 °C for 3 6 days. PMMoV, IAV, were found be stable up days after storing or °C. RSV more susceptible freeze–thaw cycles compared PMMoV viruses, which relatively Low detection IBV made difficult evaluate impact. Based on our findings, conclude that short-term transportation samples within ambient acceptable majority these viruses. Freezing even short periods recommended WBS The data obtained from study can provide guidance quality assurance purposes operational aspects surveillance.

Язык: Английский

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