Impact of Sample Storage Time and Temperature on the Stability of Respiratory Viruses and Enteric Viruses in Wastewater DOI Creative Commons
Yuanyuan Qiu,

Richardson Mah,

Logan A. Brand

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2459 - 2459

Published: Nov. 29, 2024

Wastewater-based surveillance (WBS) has been widely used to track SARS-CoV-2 as well many other viruses in communities during the COVID pandemic and post-pandemic. However, it is still not clear how temperature storage time would influence stability of wastewater. In this study, we assessed SARS-CoV-2, pepper mild mottle virus (PMMoV), influenza A (IAV) B (IBV), respiratory syncytial (RSV), enteric raw wastewater stored at room temperature, 4 °C, −20 °C for 3 6 days. PMMoV, IAV, were found be stable up days after storing or °C. RSV more susceptible freeze–thaw cycles compared PMMoV viruses, which relatively Low detection IBV made difficult evaluate impact. Based on our findings, conclude that short-term transportation samples within ambient acceptable majority these viruses. Freezing even short periods recommended WBS The data obtained from study can provide guidance quality assurance purposes operational aspects surveillance.

Language: Английский

Impact of Sample Storage Time and Temperature on the Stability of Respiratory Viruses and Enteric Viruses in Wastewater DOI Creative Commons
Yuanyuan Qiu,

Richardson Mah,

Logan A. Brand

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2459 - 2459

Published: Nov. 29, 2024

Wastewater-based surveillance (WBS) has been widely used to track SARS-CoV-2 as well many other viruses in communities during the COVID pandemic and post-pandemic. However, it is still not clear how temperature storage time would influence stability of wastewater. In this study, we assessed SARS-CoV-2, pepper mild mottle virus (PMMoV), influenza A (IAV) B (IBV), respiratory syncytial (RSV), enteric raw wastewater stored at room temperature, 4 °C, −20 °C for 3 6 days. PMMoV, IAV, were found be stable up days after storing or °C. RSV more susceptible freeze–thaw cycles compared PMMoV viruses, which relatively Low detection IBV made difficult evaluate impact. Based on our findings, conclude that short-term transportation samples within ambient acceptable majority these viruses. Freezing even short periods recommended WBS The data obtained from study can provide guidance quality assurance purposes operational aspects surveillance.

Language: Английский

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