The Science of The Total Environment, Год журнала: 2024, Номер 957, С. 177775 - 177775
Опубликована: Ноя. 30, 2024
Язык: Английский
Diagnostics, Год журнала: 2024, Номер 14(15), С. 1598 - 1598
Опубликована: Июль 25, 2024
Digital polymerase chain reaction (dPCR) has emerged as a groundbreaking technology in molecular biology and diagnostics, offering exceptional precision sensitivity nucleic acid detection quantification. This review highlights the core principles transformative potential of dPCR, particularly infectious disease diagnostics environmental surveillance. Emphasizing its evolution from traditional PCR, dPCR provides accurate absolute quantification target acids through advanced partitioning techniques. The addresses significant impact sepsis diagnosis management, showcasing superior specificity early pathogen identification drug-resistant genes. Despite advantages, challenges such optimization experimental conditions, standardization data analysis workflows, high costs are discussed. Furthermore, we compare various commercially available platforms, detailing their features applications clinical research settings. Additionally, explores dPCR's role water microbiology, wastewater surveillance monitoring waterborne pathogens, underscoring importance public health protection. In conclusion, future prospects including methodological optimization, integration with innovative technologies, expansion into new sectors like metagenomics, explored.
Язык: Английский
Процитировано
7
Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 14
Опубликована: Янв. 3, 2025
To establish a rapid detection method for canine brucellosis using recombinase-aided amplification (RAA) technology. The outer membrane protein 25 gene fragment (Omp25) of Brucella canis was targeted. Primers and fluorescent probes were designed synthesized, recombinant plasmids constructed as standards. RAA assay optimized by screening primers establishing reaction system. Sensitivity analyzed plasmid standards with varying copy numbers. Specificity tested genomes from canis, suis, melitensis, abortus, Staphylococcus aureus, pathogenic Escherichia coli, Salmonella enteritidis, Shigella spp., Proteus mirabilis, Listeria monocytogenes. Reproducibility evaluated the same different batches. system used bOmp25-F2/bOmp25-R2 probe bOmp25-P, constant temperature 39°C 15 minutes. sensitivity 1 copy/μL. No cross-reaction observed other species or bacteria, indicating high specificity. Intra-batch variability below 1.00%, inter-batch 2.00%. positive coincidence rate significantly higher than that commercial real-time fluorescence quantitative PCR (100% VS 86.96%, P<0.05). RAA-based is suitable clinical testing. It offers advantages such quick detection, sensitivity, strong specificity, good reproducibility. This provides new insights precise diagnosis pet diseases, making it promotion application.
Язык: Английский
Процитировано
0
Water, Год журнала: 2025, Номер 17(2), С. 268 - 268
Опубликована: Янв. 18, 2025
Investigating human pathogens in wastewater is crucial for identifying and predicting potential occupational health risks faced by treatment plant (WWTP) workers. This study aimed to determine the occurrence levels of Legionella pneumophila, Mycobacterium spp., Arcobacter butzleri, Aeromonas hydrophila untreated municipal wastewater. Grab influent, activated sludge, secondary settling tank (SST) effluent samples were collected bi-weekly over 6 months from 5 WWTPs Tshwane, South Africa. A. detected using quantitative PCR (qPCR), while was both a culture method qPCR. The four identified most at varying levels. pneumophila had positivity rate 92%, ranging 2 5.4 log10 MPN/100 mL. Detection rates L. serogroup 1 97%, 75%, 69%, respectively, with up 5.3 gene copies (GC)/mL. Importantly, this demonstrates molecular typing wastewater, topic that has been rarely documented. spp. all (log10 GC/mL) influent (2.8–7.6), sludge (4.8–8.9), SST (3.8–8.9) samples. butzleri 96% 82% samples, GC 0.8 6.6, 1.5 6.5, 0.7 6.6 GC/mL similar hydrophila. These findings underscore presence respiratory enteric various points, suggesting exposure WWTP emphasises need microbiological risk assessments (RAs) or reviewing existing RAs implementing necessary control measures protect
Язык: Английский
Процитировано
0
Journal of Environmental Management, Год журнала: 2025, Номер 380, С. 125125 - 125125
Опубликована: Март 31, 2025
Язык: Английский
Процитировано
0
Environmental Pollution, Год журнала: 2024, Номер unknown, С. 125051 - 125051
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
2
Environmental Research, Год журнала: 2024, Номер 263, С. 120120 - 120120
Опубликована: Окт. 9, 2024
Antibiotic resistance (AR) is a critical global health issue with significant clinical and economic implications. AR occurs when microorganisms develop mechanisms to withstand the effects of antibiotics, reducing treatment efficacy increasing risk mortality healthcare costs. While connection between antibiotic use in agricultural settings emergence well-established, role urban soils as reservoirs spreaders underexplored. This review examines complex dynamics soils, highlighting various sources including domestic wastewater, industrial effluents, practices, but also microplastics animal excrements. The selective pressure exerted by these anthropogenic promotes proliferation antibiotic-resistant bacteria, particularly through horizontal gene transfer, which facilitates transmission genes among soil environments. About that, presence antibiotics poses threat public potentially transferring human pathogens multiple pathways, direct contact, food consumption, water ingestion. Furthermore, disrupts microbial community dynamics, impacting fertility, plant growth, overall environmental quality. Therefore, this aims address gaps understanding offering insights into its implications for ecosystem integrity. By identifying suggesting evidence-based strategies, proposes valid sustainable solutions mitigate counteract spread
Язык: Английский
Процитировано
1
The Science of The Total Environment, Год журнала: 2024, Номер 955, С. 176765 - 176765
Опубликована: Окт. 10, 2024
The global spread of antibiotic resistance genes (ARGs) in the marine environment poses a significant threat to public health and natural ecosystems. This study quantified analysed distribution co-occurrence patterns ARGs wide range oceans high seas, including Atlantic, Arctic Indian Ocean, Mediterranean Sea Persian Gulf. Focusing on beta-lactamases (bla
Язык: Английский
Процитировано
1
medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Ноя. 7, 2024
Abstract Antimicrobial resistance (AMR) represents a critical global health challenge, exacerbated by the proliferation of genes in wastewater environments. This study introduces novel Resistance Persistence Index (RPI) that integrates existing risk assessment frameworks with persistence and mobility metrics antimicrobial (ARGs) treatment plant (WWTP) influent. We evaluated samples collected across wet dry seasons, focusing on dynamics ARG diversity, abundance, their associations human impact. Our findings indicate significant increase unique ARGs during season, “antibiotic inactivation” as predominant mechanism. Using Zhang et al.’s ranking framework, we assessed levels various ARGs, revealing while fewer high-risk were identified, they demonstrated substantial abundance. The RPI provided insights into how combined effects influence associated different families. integrated framework offers comprehensive tool for understanding managing AMR environmental settings, guiding future surveillance intervention strategies.
Язык: Английский
Процитировано
0
The Science of The Total Environment, Год журнала: 2024, Номер 957, С. 177775 - 177775
Опубликована: Ноя. 30, 2024
Язык: Английский
Процитировано
0