Nonsense-Mediated mRNA Decay: Mechanistic Insights and Physiological Significance

Molecular Biotechnology, Год журнала: 2023, Номер 66(11), С. 3077 - 3091

Опубликована: Ноя. 6, 2023

Язык: Английский

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Design and synthesis of novel chloropyridazine hybrids as promising anticancer agents acting by apoptosis induction and PARP-1 inhibition through a molecular hybridization strategy

RSC Medicinal Chemistry, Год журнала: 2024, Номер 15(3), С. 981 - 997

Опубликована: Янв. 1, 2024

Novel chloropyridazine hybrids as promising anticancer agents acting by apoptosis induction and PARP-1 inhibition through a molecular hybridization strategy.

Язык: Английский

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Circular RNA Pvt1 oncogene (CircPVT1) promotes the progression of papillary thyroid carcinoma by activating the Wnt/β-catenin signaling pathway and modulating the ratio of microRNA-195 (miR-195) to vascular endothelial growth factor A (VEGFA) expression

Bioengineered, Год журнала: 2021, Номер 12(2), С. 11795 - 11810

Опубликована: Дек. 19, 2021

Circular RNAs (circRNAs) have been reported to be involved in the progression of papillary thyroid carcinoma (PTC). However, role circular RNA Pvt1 oncogene (

Язык: Английский

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circHIPK3 regulates cell proliferation and migration by sponging microRNA-124 and regulating serine/threonine kinase 3 expression in esophageal squamous cell carcinoma

Bioengineered, Год журнала: 2022, Номер 13(4), С. 9767 - 9780

Опубликована: Апрель 1, 2022

Circular RNAs (circRNAs) are a type of important non-coding that widely involve in the physiological and pathophysiological process. Recent research has established link between circHIPK3 malignant activity cancer cells. However, circHIPK3' role esophageal squamous cell carcinoma (ESCC) still needs more focus. To determine prognostic value patients with ESCC, expression was quantified 32 pairs ESCC using real-time polymerase chain reaction (RT-qPCR). Then, correlation clinical characteristics also analyzed. The function development investigated biology studies bioinformatics. results showed considerably higher tumor tissues from than adjacent tissues, which associated poor prognosis. Additionally, silencing retarded proliferation, migration epithelial-mesenchymal transition (EMT) vitro, as well growth vivo. Mechanistically, we discovered behaved like sponge, absorbing microRNA-124 (miR-124) promoting serine/threonine kinase 3 (AKT3) expression. Our findings indicate acts an oncogene circHIPK3-AKT3 axis may be therapeutic target for ESCC.

Язык: Английский

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Exploration of effective biorefinery approach to obtain the commercial value-added products from algae

Sustainable Energy Technologies and Assessments, Год журнала: 2022, Номер 53, С. 102450 - 102450

Опубликована: Июнь 29, 2022

Язык: Английский

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Constructing a new prognostic signature of gastric cancer based on multiple data sets

Bioengineered, Год журнала: 2021, Номер 12(1), С. 2820 - 2835

Опубликована: Янв. 1, 2021

In order to explore new prediction methods and key genes for gastric cancer. Firstly, we downloaded the 6 original sequencing data of cancer on Illumina HumanHT-12 platform from Array Expression Gene Omnibus, used bioinformatics identify 109 up-regulated 271 down-regulated genes. Further, performed univariate Cox regression analysis prognostic-related genes, then Lasso remove collinearity, finally multivariate analyze independent prognostic (MT1M, AKR1C2, HEYL, KLK11, EEF1A2, MMP7, THBS1, KRT17, RPESP, CMTM4, UGT2B17, CGNL1, TNFRSF17, REG1A). Based these, constructed a risk proportion signature, found that patients with high-risk have high degree malignancy. Subsequently, GSE15459 set verify signature. By calculating area under recipient operator characteristic curve 5-year survival rate, test verification are 0.739 0.681, respectively, suggesting signature has moderate ability. The nomogram is visualize sig-nature, calibration showed accuracy higher. Finally, verified expression prognosis hub gene, suggested KRT17 may be potential biomarkers.

Язык: Английский

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Comprehensive Analysis of Monocarboxylate Transporter 4 (MCT4) expression in breast cancer prognosis and immune infiltration via integrated bioinformatics analysis

Bioengineered, Год журнала: 2021, Номер 12(1), С. 3850 - 3863

Опубликована: Янв. 1, 2021

Lactate blunts the anticancer immune response in breast cancer (BC). However, little is known about exact effect of lactate transporters such as monocarboxylate transporter 4 (MCT4) on immunotherapy. In this study, we investigated expression status and prognostic value MCT4 BC through large-scale transcriptome data. Our results showed that was overexpressed BC, particularly basal-like molecular subtype. Overexpression significantly correlated with high lesion grade poor prognosis. Enrichment analysis indicated MCT4-related genes were involved immune- metabolism-related bioprocesses, myeloid leukocyte activation, adaptive system, catabolic process. We also found lesions associated cell infiltration glycolytic rate-limiting enzymes like pyruvate kinase M2 (PKM2) hexokinases-3 (HK3). observations indicate may play a pivotal role maintenance tumor microenvironment (TIME) metabolic reprogramming. The glycolysis pathway (MCT4, PKM2, HK3) thus serve new targets to modulate TIME enhance immunotherapy efficiency.[Figure: see text].

Язык: Английский

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LncRNA RPSAP52 promotes cell proliferation and inhibits cell apoptosis via modulating miR-665/STAT3 in gastric cancer

Bioengineered, Год журнала: 2022, Номер 13(4), С. 8699 - 8711

Опубликована: Март 24, 2022

LncRNA RPSAP52 is a newly identified functional molecular in several cancers, but its role gastric cancer (GC) currently unclear. This study aimed to investigate the biofunction of lncRNA GC. Quantitative polymerase-chain reaction (RT-qPCR) was employed analyze gene level and miR-665. Cell proliferation capacity evaluated via CCK-8 colony formation assay. Flow cytometry applied detect cell cycle apoptosis. Hematoxylin-eosin staining conducted for histopathological analysis. Immunochemical carried out expression ki-67. Subcellular fractionation performed explore position RPSAP52. The binding relationship among RPSAP52, miR-665 STAT3 verified luciferase reporter RNA pull down experiments were used verify between Western blot. significantly elevated GC cells. Deletion restrained induced G0-G1 phase arrest, while expediting apoptosis Tumor growth vivo suppressed following depletion. MiR-665 as target A ceRNA-sponge mechanism on identified. Meanwhile, functions sponge. overexpression depletion served same exerted anti-cancer effects modulating miR-665/STAT3 GC.Abbreviations: Gastric (GC); (RT-qPCR); Helicobacter pylori (H. pylori); Roswell Park Memorial Institute 1640 (RPMI 1640); fetal bovine serum (FBS); glyceraldheyde 3-phosphate dehydrogenase (GAPDH); propidium iodide (PI); counting kit-8 (CCK-8); radioimmunoprecipitation assay (RIPA); sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); polyvinylidene fluoride (PVDF); enhanced chemiluminescence (ECL); Statistical Product Service Solutions (SPSS); standard deviation (SD).

Язык: Английский

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P53: A Key Target in the Development of Osteoarthritis

Molecular Biotechnology, Год журнала: 2023, Номер 66(1), С. 1 - 10

Опубликована: Май 8, 2023

Язык: Английский

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Deciphering the Potential Causal and Prognostic Relationships Between Gut Microbiota and Brain Tumors: Insights from Genetics Analysis and Machine Learning

Exploration, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

ABSTRACT The concept of the microbiota‐gut‐brain axis has witnessed significant advancements, and observational studies revealed dysbiosis in gut microbiota patients with brain tumors. causal relationship between tumors potential prognostic value are still unclear. Based on multiple Mendelian randomization analyses, this study confirms effects four microbes meningioma, seven pituitary tumor, eight glioma. Sherlock framework, identifies 103 meningioma‐related microbe‐related genes (MRGs), 40 tumor‐related MRGs, 63 glioma‐related MRGs expressed tissues. Almost all associated tumor grade prognosis. Lastly, model based machine learning established study, namely signature (MRS), could robustly predict prognosis glioma provide insights for immunotherapy benefits. This presents evidence tumors, which contributes to our understanding axis. prognosis, along prediction capacity MRS its association immunotherapy, provides support use as biomarkers evaluate treatment response

Язык: Английский

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