Pancreatic
cancer
(PC)
is
featured
with
low
survival
rate
and
poor
outcomes.
Herein,
we
found
that
the
expression
of
caspase-recruitment
domain-containing
protein
9
(CARD9),
predominantly
expressed
in
innate
immune
cells,
was
positively
related
to
prognosis
PC
patients.
CARD9-deficient
mice
exhibited
rapider
progression
poorer
rate.
CARD9
knockout
decreased
dendritic
cell
(DC)
maturation
impaired
DC
ability
activate
T
cells
vivo
vitro.
Adoptive
transfer
confirmed
role
deficiency
relied
on
DCs.
Creatine
identified
as
most
significant
differential
metabolite
between
WT
DCs
CARD9−/−
wherein
it
played
an
essential
maintaining
function.
led
creatine
levels
by
inhibiting
transcription
creatine-specific
transporter,
solute
carrier
family
6
member
8
(SLC6A8).
Furtherly,
deletion
blocked
p65
activation
abolishing
formation
CARD9-BCL10-MALT1
complex,
which
prevented
binding
SLC6A8
promoter.
These
events
transport
into
DCs,
immaturity
impairment
antitumor
immunity,
consequently
promoting
progression.
Frontiers in Oncology,
Год журнала:
2023,
Номер
13
Опубликована: Фев. 28, 2023
Pancreatic
ductal
adenocarcinoma
(PDAC),
the
most
prevalent
type
of
pancreatic
cancer,
is
a
highly
lethal
malignancy
with
poor
prognosis.
Polypeptide
N-acetylgalactosaminyltransferase-6
(GALNT6)
frequently
overexpressed
in
PDAC.
However,
role
GALNT6
PDAC
remains
unclear.The
expression
cancer
and
normal
tissues
were
analyzed
by
bioinformatic
analyses
immunohistochemistry.
CCK8
colony
formation
used
to
detect
cell
proliferation.
Flow
cytometry
was
applied
cycle.The
pyroptosis
detected
scanning
electron
microscopy.
The
mRNA
qRT-PCR.
protein
localization
western
blot
immunofluorescence
assay.
ELISA
levels
inflammatory
factors.The
associated
advanced
tumor
stage,
had
an
area
under
curve
(AUC)
value
0.919
based
on
genome
atlas
(TCGA)
dataset.
Knockdown
inhibited
proliferation,
migration,
invasion
cycle
arrest
cells.
Meanwhile,
knockdown
increased
interleukin-1β
(IL-1β),
interleukin-6
(IL-6),
necrosis
factor-α
(TNF-α)
interleukin-18
(IL-18),
release
inflammasome
increasing
Gasdermin
D
(GSDMD),
N-terminal
GSDMD
(GSDMD-N),
E
(GSDME)
GSDME
(GSDME-N)
suppressed
NOD-like
receptor
thermal
domain
3
(NLRP3)
glycosylation
NF-κB
inhibiting
nucleus
NF-κB.
Additionally,
promotes
degradation
O-glycosylation.We
found
that
expressed
plays
carcinogenic
role.
results
suggested
regulates
cells
through
NF-κB/NLRP3/GSDMD
signaling.
Our
study
might
provides
novel
insights
into
roles
progression.
Biomaterials Science,
Год журнала:
2023,
Номер
12(1), С. 116 - 133
Опубликована: Ноя. 3, 2023
Application
of
ATF
decorated
cisplatin
liposomes
and
anti
PD-1
antibodies
to
mice
with
pancreatic
cancer
showed
improved
efficacy
by
enhancing
drug
penetration
remodeling
the
immunosuppressive
microenvironment.
Current Issues in Molecular Biology,
Год журнала:
2025,
Номер
47(4), С. 236 - 236
Опубликована: Март 28, 2025
Background:
Our
aim
was
to
assess
the
expression
profiles
of
messenger
RNA
(mRNA)
stem-cell
genes
(POU5F1,
NANOG)
and
pancreatic
progenitor
(CK19,
HES1,
INS,
PDX1)
in
peripheral-blood
mononuclear
cells
(PBMCs)
selected
neoplastic
diseases,
such
as
cancer
neuroendocrine
tumors,
identify
disease
markers
pancreas.
Methods:
In
this
study,
49
patients
diagnosed
with
diseases
(37
12
tumors)
34
control
patients,
all
whom
were
hospitalized
at
a
tertiary
center,
enrolled.
Venous
blood
samples
collected
from
participants,
extracted
PBMCs.
The
mRNA
levels
six
markers—
POU5F1
(POU
class
5
homeobox
1),
NANOG,
CK19
(keratin
19),
HES1
(HES
family
bHLH
transcription
factor
INS
(insulin),
PDX1
(pancreatic
duodenal
1)—were
quantified
via
real-time
quantitative
PCR.
data
statistically
analyzed
explore
associations
between
gene-expression
various
clinical,
biochemical,
morphological
parameters
(including
full
count,
Ca
19-9,
weight,
height,
BMI)
Kruskal–Wallis
test,
Mann–Whitney
U
Spearman
rank
correlation
coefficient.
Results:
results
revealed
that
gene
associated
early
stem
cells,
NANOG
(median=
0.002,
p
=
0.03),
well
encoding
insulin
(median
0.004,
0.02)
0.0003,
0.005),
significantly
elevated
cancer.
However,
tumors
did
not
exhibit
significant
differences
compared
those
observed
group.
Additionally,
no
among
different
stages
Furthermore,
overexpression
found
be
positively
correlated
inflammatory
markers,
specifically
C-reactive
protein
(CRP)
WBC,
Conclusions:
An
specific
(NANOG,
CK19)
PBMCs
may
serve
potential
for
cancer,
reflecting
disease’s
interplay
systemic
inflammation.
Biomedicines,
Год журнала:
2025,
Номер
13(5), С. 1133 - 1133
Опубликована: Май 7, 2025
Oligodendroglioma
is
a
central
nervous
system
tumor
defined
by
IDH1/2
mutations
and
1p/19q
co-deletion.
Current
management
involves
maximal
resection
followed
radiotherapy/chemotherapy,
yielding
20-year
survival
rate
of
37%
for
grade
3
tumors
according
to
the
WHO
2021
classification.
As
these
primarily
affect
young
middle-aged
patients,
novel
therapies
are
urgently
needed
improve
outcomes.
Immunotherapy
has
revolutionized
treatment
modulating
immune
responses.
However,
its
application
in
oligodendrogliomas
faces
two
major
hurdles,
including
immunosuppressive
microenvironment
(TME)
blood–brain
barrier’s
restrictive
properties.
This
review
first
examines
oligodendroglioma’s
molecular
alterations
refine
diagnosis
guide
targeted
therapies.
Next,
we
focus
on
oligodendroglioma
TME
evaluate
emerging
immunotherapies,
oncolytic
viruses,
checkpoint
blockade,
chimeric
antigen
receptor
(CAR)
T-cell
therapy,
cancer
vaccines.
Finally,
discuss
current
challenges
future
directions
overcome
therapeutic
limitations
advance
strategies.
Frontiers in Oncology,
Год журнала:
2023,
Номер
13
Опубликована: Май 3, 2023
The
pathogenic
mechanisms
of
pancreatic
cancer
(PC)
are
still
not
fully
understood.
Ubiquitination
modifications
have
a
crucial
role
in
tumorigenesis
and
progression.
Yet,
the
MINDY2,
member
motif
interacting
with
Ub-containing
novel
DUB
family
(MINDY),
as
newly
identified
deubiquitinating
enzyme,
PC
is
unclear.
In
this
study,
we
found
that
MINDY2
expression
elevated
tissue
(clinical
samples)
was
associated
poor
prognosis.
We
also
pro-carcinogenic
factors
such
epithelial-mesenchymal
transition
(EMT),
inflammatory
response,
angiogenesis;
ROC
curve
suggested
has
high
diagnostic
value
PC.
Immunological
correlation
analysis
deeply
involved
immune
cell
infiltration
checkpoint-related
genes.
vivo
vitro
experiments
further
promotes
proliferation,
invasive
metastasis,
EMT.
Meanwhile,
actinin
alpha
4
(ACTN4)
MINDY2-interacting
protein
by
mass
spectrometry
other
experiments,
ACTN4
levels
were
significantly
correlated
expression.
ubiquitination
assay
confirmed
stabilizes
level
deubiquitination.
pro-oncogenic
effect
inhibited
silencing
ACTN4.
Bioinformatics
Analysis
Western
blot
through
deubiquitination
thus
activates
PI3K/AKT/mTOR
signaling
pathway.
conclusion,
oncogenic
mechanism
PC,
suggesting
viable
candidate
gene
for
may
be
therapeutic
target
critical
prognostic
indicator.