ACS ES&T Engineering,
Год журнала:
2023,
Номер
4(3), С. 717 - 727
Опубликована: Дек. 29, 2023
Extracellular
polymeric
substances
(EPSs)
in
waste
activated
sludge
(WAS)
are
important
guardians
of
active
microorganisms
against
toxic
emerging
contaminants
(ECs).
However,
how
ECs
interact
with
EPSs
and
further
interfere
microbial
functions
during
WAS
anaerobic
digestion
has
not
been
systemically
disclosed.
This
work
reported
the
dose-dependent
effects
polyhexamethylene
guanidine
(PHMG,
as
a
potential
EC)
on
methane
production
comprehensively
revealed
underlying
mechanisms.
PHMG
at
low
dose
showed
an
evident
lag
period
for
(from
0.09
d
control
to
8.23
d)
but
produced
dramatic
inhibition
high
dose.
Although
promoted
solubilization,
hydrolysis,
acidification
via
enriching
hydrolytic
acidogenic
bacteria
(i.e.,
Acidaminobacter
Acidibacter),
methanogenesis
was
inhibited
due
reduction
methanogens
Methanosaeta
Syntrophomonas)
methanogenic
activities
related
acetoclastic
hydrogenotrophic
yhdR,
pdhB,
fwdB,
frhD).
Meanwhile,
interspecies
electron
transfer
between
acidogens
decreasing
biosynthesis
shuttles
e-pili
ribA,
yigB,
ribBA,
MtrD)
reducing
mutualistic
Desulfobulbus
Methanospirillum),
which
resulted
decrease
formation.
Further
analysis
found
that
disrupted
(mainly
proteins)
improved
cell
permeability.
Then,
it
might
enter
cells
induce
formation
septal
invaginations
disturb
replication
well
metabolic
involved
biosynthesis.
functional
could
adapt
stress
significantly
recover
upregulating
genes
growth
(e.g.,
FEN1
ftsQ)
activating
quorum
sensing
system
(LuxR/I
system).
