Enhanced Stability of Lactobacillus paracasei Aspartate Ammonia-Lyase via Electrospinning for Enzyme Immobilization
Polymers,
Год журнала:
2025,
Номер
17(3), С. 270 - 270
Опубликована: Янв. 22, 2025
This
study
investigates
the
immobilization
of
Lactobacillus
paracasei
AAL
(LpAAL)
protein
onto
polyvinyl
alcohol/nylon
6/chitosan
nanofiber
membranes
using
dextran
polyaldehyde
as
a
biodegradable
cross-linker.
Immobilization
enhanced
enzyme’s
stability,
shifting
its
optimal
reaction
conditions
from
40
°C
to
45
and
pH
8.0
8.5.
While
slightly
reduced
catalytic
efficiency,
it
significantly
improved
enzyme
stability
reusability.
The
immobilized
retained
85%
initial
activity
after
7
days
storage
at
room
temperature,
compared
55%
for
free
enzyme.
Reusability
tests
demonstrated
that
LpAAL
maintained
approximately
50%
six
consecutive
cycles,
highlighting
robustness
over
repeated
use.
These
results
underscore
advantages
nanofiber-based
in
enhancing
utility
industrial
applications,
offering
practical
approach
overcoming
limitations
associated
with
systems.
Язык: Английский
Enhanced activity of enzymes encapsulated in spheres metal azolate framework-7 with defects
International Journal of Biological Macromolecules,
Год журнала:
2024,
Номер
283, С. 137689 - 137689
Опубликована: Ноя. 17, 2024
Язык: Английский
Magnetic recyclable chitosan-graphene immobilized microcystinase A: removal of microcystins from Harmful Microcystis blooms
Process Safety and Environmental Protection,
Год журнала:
2024,
Номер
191, С. 1148 - 1157
Опубликована: Сен. 12, 2024
Язык: Английский
Development of a Chitin-Based Purification System Utilizing Chitin-Binding Domain and Tobacco Etch Virus Protease Cleavage for Efficient Recombinant Protein Recovery
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 28, 2024
This
study
aims
to
develop
an
efficient
chitin-based
purification
system,
leveraging
a
novel
design
where
the
target
proteins,
superfolding
green
fluorescent
protein
(sfGFP)
and
Язык: Английский
New tools for effective production and long-term stabilization of microcystinase (MlrA) - a biotechnological perspective towards hepatotoxic microcystins remediation
Biocatalysis and Agricultural Biotechnology,
Год журнала:
2024,
Номер
61, С. 103347 - 103347
Опубликована: Сен. 6, 2024
Язык: Английский