Enhanced Stability of Lactobacillus paracasei Aspartate Ammonia-Lyase via Electrospinning for Enzyme Immobilization
Chun-Yen Hsieh,
No information about this author
Yihao Huang,
No information about this author
Yu‐Ting Yu
No information about this author
et al.
Polymers,
Journal Year:
2025,
Volume and Issue:
17(3), P. 270 - 270
Published: Jan. 22, 2025
This
study
investigates
the
immobilization
of
Lactobacillus
paracasei
AAL
(LpAAL)
protein
onto
polyvinyl
alcohol/nylon
6/chitosan
nanofiber
membranes
using
dextran
polyaldehyde
as
a
biodegradable
cross-linker.
Immobilization
enhanced
enzyme’s
stability,
shifting
its
optimal
reaction
conditions
from
40
°C
to
45
and
pH
8.0
8.5.
While
slightly
reduced
catalytic
efficiency,
it
significantly
improved
enzyme
stability
reusability.
The
immobilized
retained
85%
initial
activity
after
7
days
storage
at
room
temperature,
compared
55%
for
free
enzyme.
Reusability
tests
demonstrated
that
LpAAL
maintained
approximately
50%
six
consecutive
cycles,
highlighting
robustness
over
repeated
use.
These
results
underscore
advantages
nanofiber-based
in
enhancing
utility
industrial
applications,
offering
practical
approach
overcoming
limitations
associated
with
systems.
Language: Английский
Enhanced activity of enzymes encapsulated in spheres metal azolate framework-7 with defects
Cai Cheng,
No information about this author
Xiaoliang Guo,
No information about this author
Yu‐Qi Feng
No information about this author
et al.
International Journal of Biological Macromolecules,
Journal Year:
2024,
Volume and Issue:
283, P. 137689 - 137689
Published: Nov. 17, 2024
Language: Английский
Magnetic recyclable chitosan-graphene immobilized microcystinase A: removal of microcystins from Harmful Microcystis blooms
Cai Cheng,
No information about this author
Honglin Liu,
No information about this author
Jie Yu
No information about this author
et al.
Process Safety and Environmental Protection,
Journal Year:
2024,
Volume and Issue:
191, P. 1148 - 1157
Published: Sept. 12, 2024
Language: Английский
Development of a Chitin-Based Purification System Utilizing Chitin-Binding Domain and Tobacco Etch Virus Protease Cleavage for Efficient Recombinant Protein Recovery
Yao-Dong Lyu,
No information about this author
Po‐Ting Chen
No information about this author
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 28, 2024
This
study
aims
to
develop
an
efficient
chitin-based
purification
system,
leveraging
a
novel
design
where
the
target
proteins,
superfolding
green
fluorescent
protein
(sfGFP)
and
Language: Английский
New tools for effective production and long-term stabilization of microcystinase (MlrA) - a biotechnological perspective towards hepatotoxic microcystins remediation
Biocatalysis and Agricultural Biotechnology,
Journal Year:
2024,
Volume and Issue:
61, P. 103347 - 103347
Published: Sept. 6, 2024
Language: Английский