To
investigate
the
impact
of
sulfate
(SO42-)
on
performance
bioelectrochemical
up-flow
anaerobic
sludge
blanket
(BE-UASB),
COD/SO42-
ratio
sulfate-containing
methanolic
wastewater
was
gradually
decreased
from
20
to
3.
The
results
showed
that
BE-UASB
maintained
higher
COD
and
SO42-
removal
(i.e.,
91%
70%,
respectively)
with
methane
production
rate
278.7
mL/Lreactor/d
at
10
5,
ensuring
efficient
energy
recovery.
Sulfidogenesis
enabled
re-granulation
while
bioelectrocatalysis
further
contributed
growth/proliferation
biomass
interwoven
filaments,
rods,
spherical
microorganisms.
16S
rRNA
gene
analysis
demonstrated
promoted
diversity
methane-producing
archaea
(MPA),
including
Methanomethylovorans,
Methanosaeta,
Candidatus_Methanofastidiosum,
Methanobacterium.
Sulfate-reducing
bacteria
(SRB),
mainly
comprising
G_norank_f_Syntrophobacteraceae,
Desulfomonile,
Syntrophobacter,
tended
be
enriched
bioelectrodes,
which
not
only
eased
competition
MPA
but
also
achieved
simultaneous
in
synergy
MPA.
Water,
Год журнала:
2024,
Номер
16(11), С. 1514 - 1514
Опубликована: Май 25, 2024
Acidogenic
fermentation
is
a
technology
that
involves
halting
methanogenesis
in
the
conventional
anaerobic
digestion
process
to
produce
mainly
volatile
fatty
acids
(VFAs).
VFAs
serve
as
direct
precursors
energy-rich
or
higher
value-added
products
upon
undergoing
additional
processing.
In
this
study,
batch
reactors
were
utilized
assess
individual
and
interaction
effects
of
time
pH
variables
on
VFA
production
from
acidogenic
cassava
wastewater
through
establishment
completely
randomized
design
second-order
response
surface
(rotatable
central
composite
design),
respectively.
The
maximum
observed
was
3444.04
mg
acetic
acid
(HAc)/L
(0.58
gCODVFA/gCOD)
6
days,
with
(48.5%),
propionic
(28.3%),
butyric
(13.6%)
identified
main
metabolites.
Additionally,
assessment
effect
pH,
reached
2547.72
mgHAc/L
(0.34
at
5.9,
predominant
organic
acid.
Statistically,
individually
affect
wastewater;
however,
between
them
generated
non-significant
effect.
Bioresources and Bioprocessing,
Год журнала:
2025,
Номер
12(1)
Опубликована: Апрель 1, 2025
Abstract
The
valorization
of
vinasses
into
short-chain
fatty
acids
(SCFAs)
via
anaerobic
fermentation
(AF)
is
an
emerging
approach
that
remains
under
research.
Given
the
diverse
microbial
metabolisms
simultaneously
occurring
in
AF,
control
operational
parameters
essential
to
avoid
process
destabilization.
To
unravel
their
effect,
novelty
this
investigation
relied
on
evaluation
robustness
AF
against
perturbation
deliberately
set
(i.e.
hydraulic
retention
time
(HRT)
and
temperature
increase).
Regardless
applied
perturbation,
similar
yields
(0.5–0.6
g
COD-SCFAs/g
VS
)
were
attained.
However,
selected
perturbations
exerted
effect
microbiome
development.
Whereas
increase
mediated
a
49.70%
dissimilarity,
only
21.91%
dissimilarity
was
caused
by
HRT
increase.
Microbial
analysis
revealed
Clostridiales,
Prevotella
Megasphaera
as
key
bacteria
degradation.
bioconversion
obtained
despite
different
microbiomes
developed
after
each
suggested
functional
redundancy
highlighting
robustness.
These
findings
evidenced
feasible
biotechnology
further
valorize
vinasse
SCFAs,
demonstrating
stability
common
might
be
encountered
at
industrial
scale.
