State-of-the-art cancer biomarker detection by portable (Bio) sensing technology: A critical review

Microchemical Journal, Год журнала: 2022, Номер 177, С. 107248 - 107248

Опубликована: Фев. 9, 2022

Язык: Английский

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Aptamer-based rapid diagnosis for point-of-care application

Microfluidics and Nanofluidics, Год журнала: 2023, Номер 27(2)

Опубликована: Янв. 18, 2023

Язык: Английский

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Capacitive immunosensing at gold nanoparticle-decorated reduced graphene oxide electrodes fabricated by one-step laser nanostructuration

Biosensors and Bioelectronics, Год журнала: 2024, Номер 252, С. 116142 - 116142

Опубликована: Фев. 20, 2024

Nanostructured electrochemical biosensors have ushered in a new era of diagnostic precision, offering enhanced sensitivity and specificity for clinical biomarker detection. Among them, capacitive biosensing enables ultrasensitive label-free detection multiple molecular targets. However, the complexity cost associated with conventional fabrication methods nanostructured platforms hinder widespread adoption these devices. This study introduces biosensor that leverages laser-engraved reduced graphene oxide (rGO) electrodes decorated gold nanoparticles (AuNPs). The involves laser-scribed GO-Au

Язык: Английский

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Interleukin-6 electrochemical sensor using poly(o-phenylenediamine)-based molecularly imprinted polymer

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 404, С. 135282 - 135282

Опубликована: Янв. 5, 2024

Interleukin-6 (IL-6) is an important cytokine involved in immune responses and maintaining body homeostasis. Elevated IL-6 levels, exceeding ~40 pg/mL bodily fluids, are associated with inflammation diseases such as COVID-19, cardiovascular disorders, Alzheimer's, necessitating real-time health monitoring for personalized healthcare. In this study, we developed a highly sensitive selective sensing platform by depositing poly(o-phenylenediamine) (P(o-PD))-based molecularly imprinted polymer (MIP) onto oxygen-functionalized screen-printed carbon electrode gold nanoparticles, 3-aminopropyltriethoxysilane (APTES), glutaraldehyde (GA). While APTES enhanced the peak current due to redox probe adsorption, GA reduced it non-conductive aldehyde groups. This functionalized surface improved hydrophilicity presence of amino carbonyl We identified that 120-minute NaCl treatment effectively removed templates, ensuring successful embedding P(o-PD) matrix. calculated imprinting factor 11.2, indicating effective specific binding sites within MIP, resulting robust response IL-6. After optimizing MIP deposition through five cycles, detected concentrations ranging from 2 400 pg/mL, sensitivity 3.48 μA/log(pg/mL) limit detection 1.74 pg/mL. When tested real human serum, sensor encountered challenges matrix adsorbing other active molecules. Despite challenge, our demonstrates high selectivity long-term stability detection. These qualities position well-suited point-of-care diagnostics, emphasizing its potential reliable tool real-world applications.

Язык: Английский

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Electrochemical biosensors for point-of-care testing

Bio-Design and Manufacturing, Год журнала: 2024, Номер 7(4), С. 548 - 565

Опубликована: Июль 1, 2024

即时检验（POCT）是一种在患者身边进行疾病诊断和监测的做法，与仅在医学实验室或其他临床环境中进行的传统治疗不同。由于缺乏能够促进有效医疗测试的便携式医疗设备，POCT在最近过去较少见。然而这一领域近期呈现出增长态势，得益于诊断技术的进步、设备的小型化，以及可穿戴电子设备的快速发展。在这些发展中，电化学传感器因其高灵敏度、紧凑的尺寸并且经济实惠而在POCT领域引起了兴趣。它们被用于从疾病诊断到健康状况监测的各种应用。在本文中，我们探讨了电化学传感器的最新进展、制造它们的方法以及可以使用的各种传感机制。此外，我们还探究了如何将酶、抗体和核酸适配体等特定生物识别元素固定于电极表面，以及这些传感器在现实世界即时检验中的应用方式。

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Recent Advances of Point-of-Care Devices Integrated with Molecularly Imprinted Polymers-Based Biosensors: From Biomolecule Sensing Design to Intraoral Fluid Testing

Biosensors, Год журнала: 2022, Номер 12(3), С. 136 - 136

Опубликована: Фев. 22, 2022

Recent developments of point-of-care testing (POCT) and in vitro diagnostic medical devices have provided analytical capabilities reliable results for rapid access at or near the patient’s location. Nevertheless, challenges diagnosis still remain an important factor actual clinical trials before on-site treatment making decisions. New classes POCT depict precise technologies that can detect biomarkers biofluids such as sweat, tears, saliva urine. The introduction a novel molecularly imprinted polymer (MIP) system artificial bioreceptor could be one emerging candidates to improve performance along with physicochemical stability when used harsh environments. Here, we review potential availability MIP-based biorecognition systems custom receptors high selectivity chemical affinity specific molecules. Further progress advanced MIP technology biomolecule recognition are introduced. Finally, POCT-based system, summarized perspectives expandability periodontal future directions biosensors wearable format.

Язык: Английский

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Using Graphene-Based Biosensors to Detect Dopamine for Efficient Parkinson’s Disease Diagnostics

Biosensors, Год журнала: 2021, Номер 11(11), С. 433 - 433

Опубликована: Окт. 31, 2021

Parkinson’s disease (PD) is a neurodegenerative in which the neurotransmitter dopamine (DA) depletes due to progressive loss of nigrostriatal neurons. Therefore, DA measurement might be useful diagnostic tool for targeting early stages PD, as well helping optimize replacement therapy. Moreover, sensing appears analytical complex biological systems PD studies. To support feasibility this concept, mini-review explores currently developed graphene-based biosensors dedicated detection. We discuss various graphene modifications designed high-performance electrodes alongside their performances and interference studies, we listed based on limit detection samples. optical are also presented herein. Regarding clinical relevance, explored development trends electrochemical they relate point-of-care testing suitable site-of-location diagnostics needed personalized management. In field, into smartphone-connected intelligent However, highlighted that focus should utility rather than technical performance.

Язык: Английский

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Point-of-care electrochemical testing of biomarkers involved in inflammatory and inflammatory-associated medical conditions

Analytical and Bioanalytical Chemistry, Год журнала: 2022, Номер 415(6), С. 1033 - 1063

Опубликована: Сен. 14, 2022

Язык: Английский

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Electrochemical Immunoassay for the Detection of SARS-CoV-2 Nucleocapsid Protein in Nasopharyngeal Samples

Analytical Chemistry, Год журнала: 2022, Номер 94(11), С. 4712 - 4719

Опубликована: Март 9, 2022

Point-of-care (POC) methods currently available for detecting SARS-CoV-2 infections still lack accuracy. Here, we report the development of a highly sensitive electrochemical immunoassay capable quantitatively presence virus in patient nasopharyngeal samples using stencil-printed carbon electrodes (SPCEs) functionalized with capture antibodies targeting nucleocapsid protein (N protein). Samples are added to electrode surface, followed by horseradish peroxidase (HRP)-conjugated detection also N protein. The concentration is quantified chronoamperometry 3,3'5,5'-tetramethylbenzidine. Limits equivalent less than 50 plaque forming units/mL (PFU/mL) were determined sample volumes 20 μL. No cross-reactivity was detected influenza and other coronavirus proteins. Patient tested as part proof-of-concept clinical study where gold-standard real-time quantitative polymerase chain reaction (RT-qPCR) method. Preliminary results from data set 22 demonstrated specificity 100% (n = 9 negative according RT-qPCR) sensitivity 70% RT-PCR cycle threshold (Ct) values under 30 10) Ct 25 5), which complies World Health Organization (WHO) criteria POC COVID-19 diagnostic tests. Our SPCEs validated against standard assays, very good agreement found between both (R2 0.9993, n 6), suggesting that our assay could be used assess infectivity. takes 70 min sampling results.

Язык: Английский

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A Review on Potential Electrochemical Point-of-Care Tests Targeting Pandemic Infectious Disease Detection: COVID-19 as a Reference

Chemosensors, Год журнала: 2022, Номер 10(7), С. 269 - 269

Опубликована: Июль 11, 2022

Fast and accurate point-of-care testing (POCT) of infectious diseases is crucial for diminishing the pandemic miseries. To fight coronavirus disease 2019 (COVID-19), numerous interesting electrochemical (POC) tests have been evolved to rapidly identify causal organism SARS-CoV-2 virus, its nucleic acid antigens, antibodies patients. Many those biosensors are impressive in terms miniaturization, mass production, ease use, speed test, they could be recommended future applications pandemic-like circumstances. On other hand, self-diagnosis, sensitivity, specificity, surface chemistry, components, device configuration, portability, small analyzers, features can yet improved. Therefore, this report reviews developmental trend POC (i.e., test platforms features) reported rapid diagnosis COVID-19 correlates any significant advancements with relevant references. POCTs incorporating microfluidic/plastic chips, paper devices, nanomaterial-aided platforms, smartphone integration, epidemiological reporting attributes also surfed help preparedness. This review especially screens low-cost easily affordable setups so that management becomes faster easier. Overall, a wide-ranging package finding appropriate strategies POCT targeting detection.

Язык: Английский

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