Talanta, Год журнала: 2025, Номер 286, С. 127513 - 127513
Опубликована: Янв. 4, 2025
Язык: Английский
Molecules, Год журнала: 2023, Номер 28(13), С. 5186 - 5186
Опубликована: Июль 3, 2023
A rapid and convenient homogeneous aptamer sensor with high sensitivity is highly desirable for the electrochemical detection of tumor biomarkers. In this work, a demonstrated based on two-dimensional (2D) nanocomposite probe nanochannel modified electrode, which can realize sensitive carcinoembryonic antigen (CEA). Using π-π stacking electrostatic interaction, CEA (Apt) cationic redox (hexaammineruthenium(III), Ru(NH3)63+) are co-loaded graphite oxide (GO), leading to 2D (Ru(NH3)63+/Apt@GO). Vertically ordered mesoporous silica-nanochannel film (VMSF) easily grown supporting indium tin (ITO) electrode (VMSF/ITO) using assisted self-assembly (EASA) method within 10 s. The ultrasmall nanochannels VMSF exhibits enrichment towards Ru(NH3)63+ size exclusion material. When added in Ru(NH3)63+/Apt@GO solution, DNA recognizes binds releases be enriched detected by VMSF/ITO electrodes. Based mechanism, an ranging from 60 fg/mL 100 ng/mL limit (LOD) 14 fg/mL. Detection human serum also realized. constructed system does not require fixation recognitive surface or magnetic separation before detection, demonstrating potential applications rapid, sensing
Язык: Английский
Процитировано
23
Chemical Society Reviews, Год журнала: 2023, Номер 52(24), С. 8531 - 8579
Опубликована: Янв. 1, 2023
The framework for on-site airborne pathogen detection and transmission risk mitigation.
Язык: Английский
Процитировано
18
Lab on a Chip, Год журнала: 2024, Номер 24(9), С. 2454 - 2467
Опубликована: Янв. 1, 2024
A safe, accurate, and reliable sensing platform for urinary biomarkers analysis of is clinically important the early detection monitoring progression chronic kidney disease (CKD), as it has become one world's most prevalent non-communicable diseases.
Язык: Английский
Процитировано
8
Microchimica Acta, Год журнала: 2024, Номер 191(6)
Опубликована: Май 10, 2024
Abstract The COVID-19 pandemic underlines the need for effective strategies controlling virus spread and ensuring sensitive detection of SARS-CoV-2. This review presents potential nanomaterial-enabled optical biosensors rapid low-cost SARS-CoV-2 biomarkers, demonstrating a comprehensive analysis including colorimetric, fluorescence, surface-enhanced Raman scattering, surface plasmon resonance methods. Nanomaterials metal-based nanomaterials, metal–organic frame–based nanoparticles, nanorods, nanoporous materials, nanoshell magnetic nanoparticles employed in production are presented detail. also discusses principles, fabrication methods, nanomaterial synthesis, their applications four categories: antibody-based, antigen-based, nucleic acid–based, aptamer-based biosensors. critical includes reports published literature between years 2021 2024. In addition, offers insights into nanobiosensors diagnosis COVID-19. integration artificial intelligence machine learning technologies with is proposed to improve efficiency diagnostic systems future scenarios. Graphical
Язык: Английский
Процитировано
8
Biosensors, Год журнала: 2022, Номер 12(12), С. 1119 - 1119
Опубликована: Дек. 2, 2022
As a rapid and simple method for the detection of multiple targets, immunoassay has attracted extensive attention due to merits high specificity sensitivity. Notably, enzyme-linked immunosorbent assay (ELISA) is widely used immunoassay, which can provide sensitivity since enzyme labels promote generation catalytically amplified readouts. However, natural usually suffer from low stability, cost, difficult storage. Inspired by advantages superior tunable catalytic activities, easy preparation, nanozymes have arisen replace enzymes in immunoassay; they also possess equivalent selectivity, as well robustness. Up now, various kinds nanozymes, including mimic peroxidase, oxidase, phosphatase, been incorporated construct immunosensors. Herein, development immunoassays based on with types signals are highlighted discussed detail. Furthermore, challenges perspectives design novel widespread applications discussed.
Язык: Английский
Процитировано
27
Frontiers in Nutrition, Год журнала: 2023, Номер 9
Опубликована: Янв. 9, 2023
High affinity peptides (PTs) have been used in nanoanalysis, but there are no reports which combine PTs with a liquid crystal (LC) covalent organic framework (COF) supported soluble starch (SS) catalytic amplification system as biosensor recognition element. In this study, new, highly sensitive and selective bi-mode molecular has developed for the determination of cadmium ion (Cd 2+ ). Specifically, stable COF SS nanosol catalyst was fabricated such that nanocatalytic indicator reaction HAuCl 4 -sodium formate established based on surface-enhanced Raman scattering (SERS). The Au nanoparticles produced exhibited surface plasmon resonance (SPR) absorption peak at 535 nm SERS 1,615 cm –1 . Combining specific permitted SERS/absorption platform to be rice. linear range 0.025–0.95 nmol/L detection limit (DL) 0.012 nmol/L.
Язык: Английский
Процитировано
14
Nano Convergence, Год журнала: 2024, Номер 11(1)
Опубликована: Янв. 11, 2024
Abstract The unprecedented threat of the highly contagious virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes exponentially increased infections disease 2019 (COVID-19), highlights weak spots current diagnostic toolbox. In midst catastrophe, nanobiosensors offer a new opportunity as an alternative tool to fill gap among molecular tests, rapid antigen and serological tests. Nanobiosensors surpass potential tests because their enhanced sensitivity, thus enabling us see antigens stable easy-to-access targets. During first three years COVID-19 pandemic, substantial number studies have reported for detection SARS-CoV-2 antigens. articles on exceeds amount nanobiosensor research detecting previous infectious diseases, from influenza SARS-CoV MERS-CoV. This publishing pace also implies significance present pandemic. this review, 158 reporting are collected discuss challenges using criteria point-of-care (POC) diagnostics along with COVID-specific issues. These advances lessons during pandemic pave way preparing post-COVID era upcoming diseases. Graphical
Язык: Английский
Процитировано
6
Analytical Chemistry, Год журнала: 2022, Номер 94(10), С. 4446 - 4454
Опубликована: Март 1, 2022
The enrichment of co-reactants is one the keys to improving sensitivity electrochemiluminescence (ECL) detection. This work developed a novel hydrophobic localized strategy utilizing inner cavity β-cyclodextrin (β-CD). Pt nanoparticles (Pt NPs) were grown in situ on coordination sites for metal ions β-CD prepare β-CD-Pt nanocomposite, which could not only enrich co-reactant 3-(dibutylamino) propylamine (TDBA) highly efficiently through its but also immobilize TDBA via Pt–N bond. Meanwhile, carboxyl-functionalized poly[2,5-dioctyl-1,4-phenylene] (PDP) polymer (PNPs) as excellent ECL luminophores. With SARS-CoV-2 nucleocapsid protein (ncovNP) model protein, TDBA-β-CD-Pt nanocomposite combined PDP PNPs construct biosensor ncovNP determination. modified onto surface glassy carbon electrode (GCE) capture first antibody (Ab1) and further antigen secondary complexes (TDBA-β-CD-Pt@Ab2). resultant with sandwich structure achieved sensitive detection limit 22 fg/mL. shared an inspiration luminophore integrated provide promising platform, offering new method
Язык: Английский
Процитировано
23
Chemosensors, Год журнала: 2022, Номер 10(11), С. 459 - 459
Опубликована: Ноя. 5, 2022
COVID-19 caused by a SARS-CoV-2 infection was first reported from Wuhan, China, and later recognized as pandemic on March 11, 2020, the World Health Organization (WHO). Gold standard nucleic acid molecular-based testing have largely satisfied requirements of early diagnosis management this infectious disease; however, these techniques are expensive not readily available for point-of-care (POC) applications. The 21st century has emphasized that medicine is in dire need advanced, rapid, cheap diagnostic tools. Herein, we report molecularly imprinted polymer nanoparticles (MIP-NPs/nanoMIPs) plastic antibodies specific detection employing surface plasmon resonance (SPR) sensor. High-affinity MIP-NPs directed against were manufactured using solid-phase imprinting method. then characterized dynamic light scattering (DLS) atomic force microscopy (AFM) prior to their incorporation into label-free portable SPR device. Detection studied within range 104–106 PFU mL−1. demonstrated good binding affinity (KD = 0.12 pM) selectivity toward SARS-CoV-2. AFM, cyclic voltammetry, square-wave voltammetry studies revealed successful stepwise preparation A cross-reactivity test confirmed specificity For time, study demonstrates potential molecular technology conjunction with miniaturized devices particles high-affinity specificity. Such sensors could help monitor manage risks related virus contamination infections also beyond current pandemic.
Язык: Английский
Процитировано
23
Molecules, Год журнала: 2023, Номер 28(18), С. 6559 - 6559
Опубликована: Сен. 11, 2023
Rapid, highly sensitive, and accurate detection of tumor biomarkers in serum is great significance cancer screening, early diagnosis, postoperative monitoring. In this study, an electrochemiluminescence (ECL) immunosensing platform was constructed by enhancing the ECL signal through situ growth platinum nanoparticles (PtNPs) a nanochannel array, which can achieve sensitive marker carcinoembryonic antigen (CEA). An inexpensive readily available indium tin oxide (ITO) glass electrode used as supporting electrode, layer amino-functionalized vertically ordered mesoporous silica film (NH2-VMSF) grown on its surface using electrochemically assisted self-assembly method (EASA). The amino groups within nanochannels served anchoring sites for one-step electrodeposition PtNPs, taking advantage confinement effect ultrasmall nanochannels. After outer NH2-VMSF were derivatized with aldehyde groups, specific recognition antibodies covalently immobilized followed blocking nonspecific binding to create immunorecognition interface. acting nanocatalysts, catalyzed generation reactive oxygen species (ROS) hydrogen peroxide (H2O2), significantly luminol. exhibited high stability during continuous electrochemical scanning. When CEA specifically bound interface, resulting immune complexes restricted diffusion emitters co-reactants towards leading reduction signal. Based recognition-induced signal-gating effect, immunosensor enabled linear range 0.1 pg mL-1 1000 ng low limit (LOD, 0.03 mL-1). demonstrated excellent selectivity serum.
Язык: Английский
Процитировано
11