Cascade CRISPR/Cas12a and DSN for the electrochemical biosensing of miR-1246 in BC-derived exosomes

Bioelectrochemistry, Год журнала: 2024, Номер 159, С. 108753 - 108753

Опубликована: Май 31, 2024

Язык: Английский

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Circulating Extracellular Vesicles as Promising Biomarkers for Precession Diagnostics: A Perspective on Lung Cancer

ACS Biomaterials Science & Engineering, Год журнала: 2024, Номер 11(1), С. 95 - 134

Опубликована: Дек. 5, 2024

Extracellular vesicles (EVs) have emerged as promising biomarkers in liquid biopsy, owing to their ubiquitous presence bodily fluids and ability carry disease-related cargo. Recognizing significance disease diagnosis treatment, substantial efforts been dedicated developing efficient methods for EV isolation, detection, analysis. EVs, heterogeneous membrane-encapsulated secreted by all cells, contain bioactive substances capable of modulating recipient cell biology upon internalization, including proteins, lipids, DNA, various RNAs. Their prevalence across has positioned them pivotal mediators physiological pathological processes, notably cancer, where they hold potential straightforward tumor biomarkers. This review offers a comprehensive examination advanced nanotechnology-based techniques detecting lung cancer through It begins providing brief overview exosomes role progression. Furthermore, this explores the evolving landscape isolation cargo analysis, highlighting importance characterizing specific biomolecular signatures within EVs improved diagnostic accuracy patients. Innovative strategies enhancing sensitivity specificity integration microfluidic platforms multiplexed biosensing technologies are summarized. The discussion then extends key challenges associated with EV-based biopsies, such standardization detection protocols establishment robust analytical clinical translation. highlights transformative impact biopsy diagnosis, heralding new era personalized medicine patient care.

Язык: Английский

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Covalent organic frameworks in cancer theranostics: advancing biomarker detection and tumor-targeted therapy

Archives of Pharmacal Research, Год журнала: 2025, Номер unknown

Опубликована: Март 22, 2025

Язык: Английский

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Localized Imaging of Programmed Death-Ligand 1 on Individual Tumor-Derived Extracellular Vesicles for Prediction of Immunotherapy Response

ACS Nano, Год журнала: 2023, Номер 17(20), С. 20120 - 20134

Опубликована: Окт. 11, 2023

Programmed death-ligand 1 (PD-L1) on tumor-derived small extracellular vesicles (EVs) is a biomarker for prediction of the immunotherapy response. However, conventional bulk measurement can hardly analyze expression PD-L1 individual EVs. Herein, method localized imaging EVs (LITIE) developed. In this assay, in plasma were directly captured biochip. Then liposome-mediated membrane fusion strategy was used to image miR-21 discriminate miR-21-positive from whole populations. Subsequently, primer exchange reaction (PER) applied generate and amplified fluorescent signals identified When clinical sample tests, LITIE assay could effectively distinguish breast cancer patients healthy donors or with benign tumors. Interestingly, mice melanoma model, showed ability predict response even before drug treatment. Thus, we think measuring serve as an alternative way screening responders suitable immunotherapy.

Язык: Английский

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Laccase-inspired bi-amino acid MOFs with high substrate affinity: Catalytic deposition induced “signal-down” electrochemical response towards PD-L1

Sensors and Actuators B Chemical, Год журнала: 2023, Номер 399, С. 134773 - 134773

Опубликована: Окт. 12, 2023

Язык: Английский

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