Cascade CRISPR/Cas12a and DSN for the electrochemical biosensing of miR-1246 in BC-derived exosomes

Bioelectrochemistry, Journal Year: 2024, Volume and Issue: 159, P. 108753 - 108753

Published: May 31, 2024

Language: Английский

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Circulating Extracellular Vesicles as Promising Biomarkers for Precession Diagnostics: A Perspective on Lung Cancer

ACS Biomaterials Science & Engineering, Journal Year: 2024, Volume and Issue: 11(1), P. 95 - 134

Published: Dec. 5, 2024

Extracellular vesicles (EVs) have emerged as promising biomarkers in liquid biopsy, owing to their ubiquitous presence bodily fluids and ability carry disease-related cargo. Recognizing significance disease diagnosis treatment, substantial efforts been dedicated developing efficient methods for EV isolation, detection, analysis. EVs, heterogeneous membrane-encapsulated secreted by all cells, contain bioactive substances capable of modulating recipient cell biology upon internalization, including proteins, lipids, DNA, various RNAs. Their prevalence across has positioned them pivotal mediators physiological pathological processes, notably cancer, where they hold potential straightforward tumor biomarkers. This review offers a comprehensive examination advanced nanotechnology-based techniques detecting lung cancer through It begins providing brief overview exosomes role progression. Furthermore, this explores the evolving landscape isolation cargo analysis, highlighting importance characterizing specific biomolecular signatures within EVs improved diagnostic accuracy patients. Innovative strategies enhancing sensitivity specificity integration microfluidic platforms multiplexed biosensing technologies are summarized. The discussion then extends key challenges associated with EV-based biopsies, such standardization detection protocols establishment robust analytical clinical translation. highlights transformative impact biopsy diagnosis, heralding new era personalized medicine patient care.

Language: Английский

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Localized Imaging of Programmed Death-Ligand 1 on Individual Tumor-Derived Extracellular Vesicles for Prediction of Immunotherapy Response

ACS Nano, Journal Year: 2023, Volume and Issue: 17(20), P. 20120 - 20134

Published: Oct. 11, 2023

Programmed death-ligand 1 (PD-L1) on tumor-derived small extracellular vesicles (EVs) is a biomarker for prediction of the immunotherapy response. However, conventional bulk measurement can hardly analyze expression PD-L1 individual EVs. Herein, method localized imaging EVs (LITIE) developed. In this assay, in plasma were directly captured biochip. Then liposome-mediated membrane fusion strategy was used to image miR-21 discriminate miR-21-positive from whole populations. Subsequently, primer exchange reaction (PER) applied generate and amplified fluorescent signals identified When clinical sample tests, LITIE assay could effectively distinguish breast cancer patients healthy donors or with benign tumors. Interestingly, mice melanoma model, showed ability predict response even before drug treatment. Thus, we think measuring serve as an alternative way screening responders suitable immunotherapy.

Language: Английский

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Laccase-inspired bi-amino acid MOFs with high substrate affinity: Catalytic deposition induced “signal-down” electrochemical response towards PD-L1

Sensors and Actuators B Chemical, Journal Year: 2023, Volume and Issue: 399, P. 134773 - 134773

Published: Oct. 12, 2023

Language: Английский

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Covalent organic framework-based ratiometric electrochemical sensing platform for ultrasensitive determination of amyloid-β 42 oligomer

Talanta, Journal Year: 2024, Volume and Issue: 280, P. 126699 - 126699

Published: Aug. 13, 2024

Language: Английский

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