CRISPR integrated biosensors: A new paradigm for cancer detection

Clinica Chimica Acta, Год журнала: 2025, Номер 569, С. 120179 - 120179

Опубликована: Фев. 1, 2025

Язык: Английский

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Signal-amplification strategy and advances in electrochemistry-based CRISPR/Cas12 biosensing

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 161110 - 161110

Опубликована: Фев. 1, 2025

Язык: Английский

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CRISPR/Cas12a regulated dual-channel ratiometric fluorescence biosensing for sensitive and accurate detection of kanamycin

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113581 - 113581

Опубликована: Апрель 1, 2025

Язык: Английский

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Catalytic hairpin-assembled and DNAzyme-driven DNA walker integrated hybridization chain reaction to construct a label-free electrochemical aptasensor for the detection of acetamiprid

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 418, С. 136253 - 136253

Опубликована: Июль 4, 2024

Язык: Английский

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An Innovative and Efficient Fluorescent Detection Technique for Salmonella in Animal-Derived Foods Using the CRISPR/Cas12a-HCR System Combined with PCR/RAA

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(15), С. 8831 - 8839

Опубликована: Апрель 4, 2024

Here, we present a method for Salmonella detection using clustered regularly interspaced short palindromic repeats associated with the CRISPR-associated protein 12a-hybridization chain reaction (CRISPR/Cas12a-HCR) system combined polymerase reaction/recombinase-assisted amplification (PCR/RAA) technology. The approach relies on invA gene as biorecognition element and its through PCR RAA. In presence of target gene, Cas12a, guided by crRNA, recognizes cleaves product, initiating HCR. Fluorescently labeled single-stranded DNA (ssDNA) H1 H2 were introduced, concentration was determined based fluorescence intensity from triggered Both assays demonstrate high specificity, sensitivity, simplicity, rapidity. range 2 × 101–2 109 CFU/mL, an LOD 20 entire process enabled specific rapid within 85–105 min. Field-incurred spiked recovery tests conducted in mutton beef samples both assays, demonstrating satisfactory accuracy animal-derived foods. By combining CRISPR/Cas12a hybridization technology, this study presents sensitive that is crucial identifying pathogenic bacteria monitoring food safety.

Язык: Английский

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Dual electrochemical signal “signal-on-off” sensor based on CHA-Td-HCR and CRISPR-Cas12a for MUC1 detection

Talanta, Год журнала: 2024, Номер 279, С. 126665 - 126665

Опубликована: Авг. 3, 2024

Язык: Английский

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CRISPR/Cas12a-triggered ordered concatemeric DNA probes signal-on/off multifunctional analytical sensing system for ultrasensitive detection of thalassemia

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 276, С. 133884 - 133884

Опубликована: Июль 14, 2024

Язык: Английский

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A MXene-Au Nanosheets-based Fluorescent-SERS Dual-Mode Biosensor Integrated with CRISPR/Cas12a System for Endotoxin Detection

Sensors and Actuators B Chemical, Год журнала: 2024, Номер unknown, С. 137120 - 137120

Опубликована: Дек. 1, 2024

Язык: Английский

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Biosensors for detection of hormones and growth factors

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 181 - 213

Опубликована: Янв. 1, 2025

Язык: Английский

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Exploring a New Ruthenium(II) Complex with High DNA Binding Ability as a Novel Efficient Luminescent Intercalation Agent to Construct a Label-Free ECL/PL Dual-Mode Biosensor

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 25, 2025

Ruthenium(II) complexes with special ligands have been widely recognized in numerous fields and attributed to their outstanding DNA binding capacity. Hybridization chain reaction (HCR), as an enzyme-free amplification technique, forms long double-stranded (dsDNA) structures, which provides intercalation platform for these obtains effective enhancement of luminescent signals a significant extent enhances the sensitivity detection. Hence, Ru(dip)2(tpphz) [dip = 4,7-diphenyl-1,10-phenanthroline, tpphz tetrapyrido[3,2-a:2′,3′-c:3″,2″-h:2‴,3‴-j]phenazine] confirmed possess high capacity via UV–vis absorption spectroscopy AutoDock theoretical simulation calculations was synthesized luminescence probe. As proof concept, label-free ECL/PL dual-mode biosensor further constructed. In this design, magnetic silica spheres trigger were amplified by HCR hairpin DNA, forming large amounts dsDNA on surface, incorporated generate robust signals. Trigger cleaved owing activation Cas12a cleavage ability presence target, disappeared, reduced. The exhibited selectivity, LOD low 69 fM (S/N 3). results proved that has excellent ECL PL dual properties, huge potential establish biosensors simultaneously offers tremendous prospect anticancer, gene therapy, molecular probes beyond future.

Язык: Английский

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